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A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes

(2008) PLANT PHYSIOLOGY. 148(1). p.358-368
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Abstract
During the interaction between sedentary plant-parasitic nematodes and their host, complex morphological and physiological changes occur in the infected plant tissue, finally resulting in the establishment of a nematode feeding site. This cellular transformation is the result of altered plant gene expression most likely induced by proteins injected in the plant cell by the nematode. Here, we report on the identification of a WRKY transcription factor expressed during nematode infection. Using both promoter-reporter gene fusions and in situ reverse transcription-polymerase chain reaction, we could show that AtWRKY23 is expressed during the early stages of feeding site establishment. Knocking down the expression of WRKY23 resulted in lower infection of the cyst nematode Heterodera schachtii. WRKY23 is an auxin-inducible gene and in uninfected plants WRKY23 acts downstream of the Aux/IAA protein SLR/IAA14. Although auxin is known to be involved in feeding site formation, our results suggest that, during early stages, auxin-independent signals might be at play to activate the initial expression of WRKY23.
Keywords
DEFENSE RESPONSE, LEAF SENESCENCE, PSEUDOMONAS-SYRINGAE, GENE-EXPRESSION, ARABIDOPSIS-THALIANA, ROOT-KNOT NEMATODES, WRKY TRANSCRIPTION FACTORS, DNA-BINDING, AUXIN, ACID

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Chicago
Grunewald, Wim, Mansour Karimi, Krzysztof Wieczorek, Elke Van de Cappelle, Elisabeth Wischnitzki, Florian Grundler, Dirk Inzé, Tom Beeckman, and Godelieve Gheysen. 2008. “A Role for AtWRKY23 in Feeding Site Establishment of Plant-parasitic Nematodes.” Plant Physiology 148 (1): 358–368.
APA
Grunewald, W., Karimi, M., Wieczorek, K., Van de Cappelle, E., Wischnitzki, E., Grundler, F., Inzé, D., et al. (2008). A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes. PLANT PHYSIOLOGY, 148(1), 358–368.
Vancouver
1.
Grunewald W, Karimi M, Wieczorek K, Van de Cappelle E, Wischnitzki E, Grundler F, et al. A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes. PLANT PHYSIOLOGY. 2008;148(1):358–68.
MLA
Grunewald, Wim, Mansour Karimi, Krzysztof Wieczorek, et al. “A Role for AtWRKY23 in Feeding Site Establishment of Plant-parasitic Nematodes.” PLANT PHYSIOLOGY 148.1 (2008): 358–368. Print.
@article{440992,
  abstract     = {During the interaction between sedentary plant-parasitic nematodes and their host, complex morphological and physiological changes occur in the infected plant tissue, finally resulting in the establishment of a nematode feeding site. This cellular transformation is the result of altered plant gene expression most likely induced by proteins injected in the plant cell by the nematode. Here, we report on the identification of a WRKY transcription factor expressed during nematode infection. Using both promoter-reporter gene fusions and in situ reverse transcription-polymerase chain reaction, we could show that AtWRKY23 is expressed during the early stages of feeding site establishment. Knocking down the expression of WRKY23 resulted in lower infection of the cyst nematode Heterodera schachtii. WRKY23 is an auxin-inducible gene and in uninfected plants WRKY23 acts downstream of the Aux/IAA protein SLR/IAA14. Although auxin is known to be involved in feeding site formation, our results suggest that, during early stages, auxin-independent signals might be at play to activate the initial expression of WRKY23.},
  author       = {Grunewald, Wim and Karimi, Mansour and Wieczorek, Krzysztof and Van de Cappelle, Elke and Wischnitzki, Elisabeth and Grundler, Florian and Inz{\'e}, Dirk and Beeckman, Tom and Gheysen, Godelieve},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {DEFENSE RESPONSE,LEAF SENESCENCE,PSEUDOMONAS-SYRINGAE,GENE-EXPRESSION,ARABIDOPSIS-THALIANA,ROOT-KNOT NEMATODES,WRKY TRANSCRIPTION FACTORS,DNA-BINDING,AUXIN,ACID},
  language     = {eng},
  number       = {1},
  pages        = {358--368},
  title        = {A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes},
  url          = {http://dx.doi.org/10.1104/pp.108.119131},
  volume       = {148},
  year         = {2008},
}

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