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A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes

Wim Grunewald UGent, Mansour Karimi UGent, Krzysztof Wieczorek, Elke Van de Cappelle, Elisabeth Wischnitzki UGent, Florian Grundler, Dirk Inzé UGent, Tom Beeckman UGent and Godelieve Gheysen UGent (2008) PLANT PHYSIOLOGY. 148(1). p.358-368
abstract
During the interaction between sedentary plant-parasitic nematodes and their host, complex morphological and physiological changes occur in the infected plant tissue, finally resulting in the establishment of a nematode feeding site. This cellular transformation is the result of altered plant gene expression most likely induced by proteins injected in the plant cell by the nematode. Here, we report on the identification of a WRKY transcription factor expressed during nematode infection. Using both promoter-reporter gene fusions and in situ reverse transcription-polymerase chain reaction, we could show that AtWRKY23 is expressed during the early stages of feeding site establishment. Knocking down the expression of WRKY23 resulted in lower infection of the cyst nematode Heterodera schachtii. WRKY23 is an auxin-inducible gene and in uninfected plants WRKY23 acts downstream of the Aux/IAA protein SLR/IAA14. Although auxin is known to be involved in feeding site formation, our results suggest that, during early stages, auxin-independent signals might be at play to activate the initial expression of WRKY23.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DEFENSE RESPONSE, LEAF SENESCENCE, PSEUDOMONAS-SYRINGAE, GENE-EXPRESSION, ARABIDOPSIS-THALIANA, ROOT-KNOT NEMATODES, WRKY TRANSCRIPTION FACTORS, DNA-BINDING, AUXIN, ACID
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
148
issue
1
pages
358 - 368
Web of Science type
Article
Web of Science id
000258947600032
JCR category
PLANT SCIENCES
JCR impact factor
6.11 (2008)
JCR rank
12/155 (2008)
JCR quartile
1 (2008)
ISSN
0032-0889
DOI
10.1104/pp.108.119131
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
440992
handle
http://hdl.handle.net/1854/LU-440992
date created
2008-11-18 08:09:00
date last changed
2012-12-17 13:41:57
@article{440992,
  abstract     = {During the interaction between sedentary plant-parasitic nematodes and their host, complex morphological and physiological changes occur in the infected plant tissue, finally resulting in the establishment of a nematode feeding site. This cellular transformation is the result of altered plant gene expression most likely induced by proteins injected in the plant cell by the nematode. Here, we report on the identification of a WRKY transcription factor expressed during nematode infection. Using both promoter-reporter gene fusions and in situ reverse transcription-polymerase chain reaction, we could show that AtWRKY23 is expressed during the early stages of feeding site establishment. Knocking down the expression of WRKY23 resulted in lower infection of the cyst nematode Heterodera schachtii. WRKY23 is an auxin-inducible gene and in uninfected plants WRKY23 acts downstream of the Aux/IAA protein SLR/IAA14. Although auxin is known to be involved in feeding site formation, our results suggest that, during early stages, auxin-independent signals might be at play to activate the initial expression of WRKY23.},
  author       = {Grunewald, Wim and Karimi, Mansour and Wieczorek, Krzysztof and Van de Cappelle, Elke and Wischnitzki, Elisabeth and Grundler, Florian and Inz{\'e}, Dirk and Beeckman, Tom and Gheysen, Godelieve},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {DEFENSE RESPONSE,LEAF SENESCENCE,PSEUDOMONAS-SYRINGAE,GENE-EXPRESSION,ARABIDOPSIS-THALIANA,ROOT-KNOT NEMATODES,WRKY TRANSCRIPTION FACTORS,DNA-BINDING,AUXIN,ACID},
  language     = {eng},
  number       = {1},
  pages        = {358--368},
  title        = {A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes},
  url          = {http://dx.doi.org/10.1104/pp.108.119131},
  volume       = {148},
  year         = {2008},
}

Chicago
Grunewald, Wim, Mansour Karimi, Krzysztof Wieczorek, Elke Van de Cappelle, Elisabeth Wischnitzki, Florian Grundler, Dirk Inzé, Tom Beeckman, and Godelieve Gheysen. 2008. “A Role for AtWRKY23 in Feeding Site Establishment of Plant-parasitic Nematodes.” Plant Physiology 148 (1): 358–368.
APA
Grunewald, W., Karimi, M., Wieczorek, K., Van de Cappelle, E., Wischnitzki, E., Grundler, F., Inzé, D., et al. (2008). A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes. PLANT PHYSIOLOGY, 148(1), 358–368.
Vancouver
1.
Grunewald W, Karimi M, Wieczorek K, Van de Cappelle E, Wischnitzki E, Grundler F, et al. A role for AtWRKY23 in feeding site establishment of plant-parasitic nematodes. PLANT PHYSIOLOGY. 2008;148(1):358–68.
MLA
Grunewald, Wim, Mansour Karimi, Krzysztof Wieczorek, et al. “A Role for AtWRKY23 in Feeding Site Establishment of Plant-parasitic Nematodes.” PLANT PHYSIOLOGY 148.1 (2008): 358–368. Print.