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Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants

(2008) PLANT PHYSIOLOGY. 148(2). p.960-968
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Abstract
Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate between free radical (type I)- and singlet oxygen (O-1(2); type II)- mediated lipid peroxidation (LPO) signatures by using hydroxy fatty acids as specific reporters. Using this method, we observed that in nonphotosynthesizing Arabidopsis (Arabidopsis thaliana) tissues, nonenzymatic LPO was almost exclusively catalyzed by free radicals both under normal and oxidative stress conditions. However, in leaf tissues under optimal growth conditions, O-1(2) was responsible for more than 80% of the nonenzymatic LPO. In Arabidopsis mutants favoring O-1(2) production, photooxidative stress led to a dramatic increase of O-1(2) (type II) LPO that preceded cell death. Furthermore, under all conditions and in mutants that favor the production of superoxide and hydrogen peroxide (two sources for type I LPO reactions), plant cell death was nevertheless always preceded by an increase in O-1(2)-dependent (type II) LPO. Thus, besides triggering a genetic cell death program, as demonstrated previously with the Arabidopsis fluorescent mutant, O-1(2) plays a major destructive role during the execution of reactive oxygen species-induced cell death in leaf tissues.
Keywords
CHLOROPLAST, TOBACCO, RESPONSES, GENE-EXPRESSION, CHLAMYDOMONAS-REINHARDTII, CELL-DEATH, ARABIDOPSIS-THALIANA, OXIDATIVE STRESS, PHYTOPROSTANES, PHOTOSYNTHESIS

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Chicago
Triantaphylidès, Christian, Markus Krischke, Frank Hoeberichts, Brigitte Ksas, Gabriele Gresser, Michel Havaux, Frank Van Breusegem, and Martin Johannes Mueller. 2008. “Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants.” Plant Physiology 148 (2): 960–968.
APA
Triantaphylidès, C., Krischke, M., Hoeberichts, F., Ksas, B., Gresser, G., Havaux, M., Van Breusegem, F., et al. (2008). Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants. PLANT PHYSIOLOGY, 148(2), 960–968.
Vancouver
1.
Triantaphylidès C, Krischke M, Hoeberichts F, Ksas B, Gresser G, Havaux M, et al. Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants. PLANT PHYSIOLOGY. 2008;148(2):960–8.
MLA
Triantaphylidès, Christian, Markus Krischke, Frank Hoeberichts, et al. “Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants.” PLANT PHYSIOLOGY 148.2 (2008): 960–968. Print.
@article{440967,
  abstract     = {Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate between free radical (type I)- and singlet oxygen (O-1(2); type II)- mediated lipid peroxidation (LPO) signatures by using hydroxy fatty acids as specific reporters. Using this method, we observed that in nonphotosynthesizing Arabidopsis (Arabidopsis thaliana) tissues, nonenzymatic LPO was almost exclusively catalyzed by free radicals both under normal and oxidative stress conditions. However, in leaf tissues under optimal growth conditions, O-1(2) was responsible for more than 80% of the nonenzymatic LPO. In Arabidopsis mutants favoring O-1(2) production, photooxidative stress led to a dramatic increase of O-1(2) (type II) LPO that preceded cell death. Furthermore, under all conditions and in mutants that favor the production of superoxide and hydrogen peroxide (two sources for type I LPO reactions), plant cell death was nevertheless always preceded by an increase in O-1(2)-dependent (type II) LPO. Thus, besides triggering a genetic cell death program, as demonstrated previously with the Arabidopsis fluorescent mutant, O-1(2) plays a major destructive role during the execution of reactive oxygen species-induced cell death in leaf tissues.},
  author       = {Triantaphylidès, Christian and Krischke, Markus and Hoeberichts, Frank and Ksas, Brigitte and Gresser, Gabriele and Havaux, Michel and Van Breusegem, Frank and Mueller, Martin Johannes},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keywords     = {CHLOROPLAST,TOBACCO,RESPONSES,GENE-EXPRESSION,CHLAMYDOMONAS-REINHARDTII,CELL-DEATH,ARABIDOPSIS-THALIANA,OXIDATIVE STRESS,PHYTOPROSTANES,PHOTOSYNTHESIS},
  language     = {eng},
  number       = {2},
  pages        = {960--968},
  title        = {Singlet oxygen is the major reactive oxygen species involved in photooxidative damage to plants},
  url          = {http://dx.doi.org/10.1104/pp.108.125690},
  volume       = {148},
  year         = {2008},
}

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