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Dioctadecyldimethylammonium:monoolein nanocarriers for efficient in vitro gene silencing

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NB-Photonics
Abstract
This study describes a novel liposomal formulation for siRNA delivery, based on the mixture of the neutral lipid mono olein (MO) and cationic lipids of the dioctadecyldimethylammonium (DODA) family. The cationic lipids dioctadecyldimethylammonium bromide (DODAB) and chloride (DODAC) were compared in order to identify which one will most efficiently induce gene silencing. MO has a fluidizing effect on DODAC and DODAB liposomes, although it was more homogeneously distributed in DODAC bilayers. All MO-based liposomal formulations were able to efficiently encapsulate siRNA. Stable lipoplexes of small size (100-160 nm) with a positive surface charge (>+45 mV) were formed. A more uniform MO incorporation in DODAC:MO may explain an increase of the fusogenic potential of these liposomes. The siRNA-lipoplexes were readily internalized by human nonsmall cell lung carcinoma (H1299) cells, in an energy dependent process. DODAB:MO nanocarriers showed a higher internalization efficiency in comparison to DODAC:MO lipoplexes, and were also more efficient in promoting gene silencing. MO had a similar gene silencing ability as the commonly used helper lipid 1,2-dioleyl-3-phosphatidylethanolamine (DOPE), but with much lower cytotoxicity. Taking in consideration all the results presented, DODAB:MO liposomes are the most promising tested formulation for systemic siRNA delivery.
Keywords
MECHANISM, VESICLES, MEMBRANE-FUSION, INTERFERING RNA, MAMMALIAN-CELLS, DEXTRAN NANOGELS, CATIONIC LIPOSOMES, PHASE-TRANSITION, TRANSFECTION EFFICIENCY, SIRNA DELIVERY, siRNA delivery, monoolein, liposomes, gene silencing, counterion

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Citation

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MLA
Oliveira, Ana Cristina Norbert, Thomas Martens, Koen Raemdonck, et al. “Dioctadecyldimethylammonium:monoolein Nanocarriers for Efficient in Vitro Gene Silencing.” ACS APPLIED MATERIALS & INTERFACES 6.9 (2014): 6977–6989. Print.
APA
Oliveira, A. C. N., Martens, T., Raemdonck, K., Adati, R. D., Feitosae, E., Botelho, C., Castro Gomes, A., et al. (2014). Dioctadecyldimethylammonium:monoolein nanocarriers for efficient in vitro gene silencing. ACS APPLIED MATERIALS & INTERFACES, 6(9), 6977–6989.
Chicago author-date
Oliveira, Ana Cristina Norbert, Thomas Martens, Koen Raemdonck, Renata Danielle Adati, Eloi Feitosae, Cláudia Botelho, Andreia Castro Gomes, Kevin Braeckmans, and María Elisabete Cunha Dias Real Oliveira. 2014. “Dioctadecyldimethylammonium:monoolein Nanocarriers for Efficient in Vitro Gene Silencing.” Acs Applied Materials & Interfaces 6 (9): 6977–6989.
Chicago author-date (all authors)
Oliveira, Ana Cristina Norbert, Thomas Martens, Koen Raemdonck, Renata Danielle Adati, Eloi Feitosae, Cláudia Botelho, Andreia Castro Gomes, Kevin Braeckmans, and María Elisabete Cunha Dias Real Oliveira. 2014. “Dioctadecyldimethylammonium:monoolein Nanocarriers for Efficient in Vitro Gene Silencing.” Acs Applied Materials & Interfaces 6 (9): 6977–6989.
Vancouver
1.
Oliveira ACN, Martens T, Raemdonck K, Adati RD, Feitosae E, Botelho C, et al. Dioctadecyldimethylammonium:monoolein nanocarriers for efficient in vitro gene silencing. ACS APPLIED MATERIALS & INTERFACES. 2014;6(9):6977–89.
IEEE
[1]
A. C. N. Oliveira et al., “Dioctadecyldimethylammonium:monoolein nanocarriers for efficient in vitro gene silencing,” ACS APPLIED MATERIALS & INTERFACES, vol. 6, no. 9, pp. 6977–6989, 2014.
@article{4408456,
  abstract     = {This study describes a novel liposomal formulation for siRNA delivery, based on the mixture of the neutral lipid mono olein (MO) and cationic lipids of the dioctadecyldimethylammonium (DODA) family. The cationic lipids dioctadecyldimethylammonium bromide (DODAB) and chloride (DODAC) were compared in order to identify which one will most efficiently induce gene silencing. MO has a fluidizing effect on DODAC and DODAB liposomes, although it was more homogeneously distributed in DODAC bilayers. All MO-based liposomal formulations were able to efficiently encapsulate siRNA. Stable lipoplexes of small size (100-160 nm) with a positive surface charge (>+45 mV) were formed. A more uniform MO incorporation in DODAC:MO may explain an increase of the fusogenic potential of these liposomes. The siRNA-lipoplexes were readily internalized by human nonsmall cell lung carcinoma (H1299) cells, in an energy dependent process. DODAB:MO nanocarriers showed a higher internalization efficiency in comparison to DODAC:MO lipoplexes, and were also more efficient in promoting gene silencing. MO had a similar gene silencing ability as the commonly used helper lipid 1,2-dioleyl-3-phosphatidylethanolamine (DOPE), but with much lower cytotoxicity. Taking in consideration all the results presented, DODAB:MO liposomes are the most promising tested formulation for systemic siRNA delivery.},
  author       = {Oliveira, Ana Cristina Norbert and Martens, Thomas and Raemdonck, Koen and Adati, Renata Danielle and Feitosae, Eloi and Botelho, Cláudia and Castro Gomes, Andreia and Braeckmans, Kevin and Cunha Dias Real Oliveira, María Elisabete },
  issn         = {1944-8244},
  journal      = {ACS APPLIED MATERIALS & INTERFACES},
  keywords     = {MECHANISM,VESICLES,MEMBRANE-FUSION,INTERFERING RNA,MAMMALIAN-CELLS,DEXTRAN NANOGELS,CATIONIC LIPOSOMES,PHASE-TRANSITION,TRANSFECTION EFFICIENCY,SIRNA DELIVERY,siRNA delivery,monoolein,liposomes,gene silencing,counterion},
  language     = {eng},
  number       = {9},
  pages        = {6977--6989},
  title        = {Dioctadecyldimethylammonium:monoolein nanocarriers for efficient in vitro gene silencing},
  url          = {http://dx.doi.org/10.1021/am500793y},
  volume       = {6},
  year         = {2014},
}

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