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Isolation and characterization of Helicobacter suis sp nov from pig stomachs

Margo Baele UGent, Annemie Decostere UGent, Peter Vandamme UGent, Liesbeth Ceelen, Ann Hellemans, J Mast, Koen Chiers UGent, Richard Ducatelle UGent and Freddy Haesebrouck UGent (2008) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 58(6). p.1350-1358
abstract
A new cultivation method was successfully applied for the in vitro isolation of a hitherto uncultured spiral Helicobacter species associated with ulceration of the non-glandular stomach and gastritis in pigs and formerly described as 'Candidatus Helicobacter suis'. Three isolates, HS1(T), HS2 and HS3, were subcultured from the stomach mucosa of three pigs after slaughter and were analysed using a polyphasic taxonomic approach. The novel isolates grew on biphasic culture plates or very moist agar bases in microaerobic conditions and exhibited urease, oxidase and catalase activities. Sequencing of the 16S rRNA gene, the 23S rRNA gene, the partial hsp60 gene and partial ureAB genes confirmed that the strains present in the gastric mucosa of pigs constituted a separate taxon, corresponding to 'Helicobacter heilmannii' type 1 strains as detected in the gastric mucosa of humans and other primates. For all genes sequenced, the highest sequence similarities were obtained with Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis, Helicobacter species isolated from the gastric mucosa of dogs and cats, which have also been detected in the human gastric mucosa and which are commonly referred to as 'Helicobacter heilmannii' type 2. SDS-PAGE of whole-cell proteins of strains HS1(T), HS2 and HS3 differentiated them from other Helicobacter species of gastric origin. The results of the polyphasic taxonomic analysis confirmed that the novel isolates constitute a novel taxon corresponding to 'Helicobacter heilmannii' type 1 strains from humans and to 'Candidatus H. suis' from pigs. The name Helicobacter suis sp. nov. is proposed for the novel isolates with the type strain HS1(T) (=LMG 23995(T) =DSM 19735(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PORCINE GASTRIC-MUCOSA, 16S RIBOSOMAL-RNA, SPIRAL MICROORGANISM, GASTROSPIRILLUM-SUIS, GENUS HELICOBACTER, HEILMANNII, IDENTIFICATION, BIZZOZERONII, SAMPLES, PYLORI
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
58
issue
6
pages
1350 - 1358
Web of Science type
Article
Web of Science id
000257277200014
JCR category
MICROBIOLOGY
JCR impact factor
1.463 (2008)
JCR rank
68/91 (2008)
JCR quartile
3 (2008)
ISSN
1466-5026
DOI
10.1099/ijs.0.65133-0
language
English
UGent publication?
yes
classification
A1
id
440370
handle
http://hdl.handle.net/1854/LU-440370
date created
2008-11-17 12:36:00
date last changed
2015-06-17 11:06:24
@article{440370,
  abstract     = {A new cultivation method was successfully applied for the in vitro isolation of a hitherto uncultured spiral Helicobacter species associated with ulceration of the non-glandular stomach and gastritis in pigs and formerly described as 'Candidatus Helicobacter suis'. Three isolates, HS1(T), HS2 and HS3, were subcultured from the stomach mucosa of three pigs after slaughter and were analysed using a polyphasic taxonomic approach. The novel isolates grew on biphasic culture plates or very moist agar bases in microaerobic conditions and exhibited urease, oxidase and catalase activities. Sequencing of the 16S rRNA gene, the 23S rRNA gene, the partial hsp60 gene and partial ureAB genes confirmed that the strains present in the gastric mucosa of pigs constituted a separate taxon, corresponding to 'Helicobacter heilmannii' type 1 strains as detected in the gastric mucosa of humans and other primates. For all genes sequenced, the highest sequence similarities were obtained with Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis, Helicobacter species isolated from the gastric mucosa of dogs and cats, which have also been detected in the human gastric mucosa and which are commonly referred to as 'Helicobacter heilmannii' type 2. SDS-PAGE of whole-cell proteins of strains HS1(T), HS2 and HS3 differentiated them from other Helicobacter species of gastric origin. The results of the polyphasic taxonomic analysis confirmed that the novel isolates constitute a novel taxon corresponding to 'Helicobacter heilmannii' type 1 strains from humans and to 'Candidatus H. suis' from pigs. The name Helicobacter suis sp. nov. is proposed for the novel isolates with the type strain HS1(T) (=LMG 23995(T) =DSM 19735(T)).},
  author       = {Baele, Margo and Decostere, Annemie and Vandamme, Peter and Ceelen, Liesbeth and Hellemans, Ann and Mast, J and Chiers, Koen and Ducatelle, Richard and Haesebrouck, Freddy},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {PORCINE GASTRIC-MUCOSA,16S RIBOSOMAL-RNA,SPIRAL MICROORGANISM,GASTROSPIRILLUM-SUIS,GENUS HELICOBACTER,HEILMANNII,IDENTIFICATION,BIZZOZERONII,SAMPLES,PYLORI},
  language     = {eng},
  number       = {6},
  pages        = {1350--1358},
  title        = {Isolation and characterization of Helicobacter suis sp nov from pig stomachs},
  url          = {http://dx.doi.org/10.1099/ijs.0.65133-0},
  volume       = {58},
  year         = {2008},
}

Chicago
Baele, Margo, Annemie Decostere, Peter Vandamme, Liesbeth Ceelen, Ann Hellemans, J Mast, Koen Chiers, Richard Ducatelle, and Freddy Haesebrouck. 2008. “Isolation and Characterization of Helicobacter Suis Sp Nov from Pig Stomachs.” International Journal of Systematic and Evolutionary Microbiology 58 (6): 1350–1358.
APA
Baele, M., Decostere, A., Vandamme, P., Ceelen, L., Hellemans, A., Mast, J., Chiers, K., et al. (2008). Isolation and characterization of Helicobacter suis sp nov from pig stomachs. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 58(6), 1350–1358.
Vancouver
1.
Baele M, Decostere A, Vandamme P, Ceelen L, Hellemans A, Mast J, et al. Isolation and characterization of Helicobacter suis sp nov from pig stomachs. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2008;58(6):1350–8.
MLA
Baele, Margo, Annemie Decostere, Peter Vandamme, et al. “Isolation and Characterization of Helicobacter Suis Sp Nov from Pig Stomachs.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 58.6 (2008): 1350–1358. Print.