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On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

(2014) NANOSCALE. 6(3). p.1741-1747
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.
Keywords
DEEP, PROGRESSION, COMMUNICATION, MEDIATORS, BIOMARKERS, MICROSCOPY, MICROVESICLES, TUMOR-GROWTH, SINGLE-PARTICLE TRACKING, PLANE ILLUMINATION

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Citation

Please use this url to cite or link to this publication:

Chicago
Deschout, Hendrik, Koen Raemdonck, Stephan Stremersch, Pietro Maoddi, Guillaume Mernier, Philippe Renaud, Sebastien Jiguet, et al. 2014. “On-chip Light Sheet Illumination Enables Diagnostic Size and Concentration Measurements of Membrane Vesicles in Biofluids.” Nanoscale 6 (3): 1741–1747.
APA
Deschout, H., Raemdonck, K., Stremersch, S., Maoddi, P., Mernier, G., Renaud, P., Jiguet, S., et al. (2014). On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids. NANOSCALE, 6(3), 1741–1747.
Vancouver
1.
Deschout H, Raemdonck K, Stremersch S, Maoddi P, Mernier G, Renaud P, et al. On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids. NANOSCALE. 2014;6(3):1741–7.
MLA
Deschout, Hendrik, Koen Raemdonck, Stephan Stremersch, et al. “On-chip Light Sheet Illumination Enables Diagnostic Size and Concentration Measurements of Membrane Vesicles in Biofluids.” NANOSCALE 6.3 (2014): 1741–1747. Print.
@article{4396640,
  abstract     = {Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.},
  author       = {Deschout, Hendrik and Raemdonck, Koen and Stremersch, Stephan and Maoddi, Pietro and Mernier, Guillaume and Renaud, Philippe and Jiguet, Sebastien and Hendrix, An and Bracke, Marc and Van den Broecke, Rudy and R{\"o}ding, Magnus and Rudemo, Mats and Demeester, Jo and De Smedt, Stefaan and Strubbe, Filip and Neyts, Kristiaan and Braeckmans, Kevin},
  issn         = {2040-3364},
  journal      = {NANOSCALE},
  keyword      = {DEEP,PROGRESSION,COMMUNICATION,MEDIATORS,BIOMARKERS,MICROSCOPY,MICROVESICLES,TUMOR-GROWTH,SINGLE-PARTICLE TRACKING,PLANE ILLUMINATION},
  language     = {eng},
  number       = {3},
  pages        = {1741--1747},
  title        = {On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids},
  url          = {http://dx.doi.org/10.1039/c3nr04432g},
  volume       = {6},
  year         = {2014},
}

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