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FRAP in pharmaceutical research: practical guidelines and applications in drug delivery

Hendrik Deschout (UGent) , Koen Raemdonck (UGent) , Jo Demeester (UGent) , Stefaan De Smedt (UGent) and Kevin Braeckmans (UGent)
(2014) PHARMACEUTICAL RESEARCH. 31(2). p.255-270
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Abstract
Fluorescence recovery after photobleaching (FRAP) is a fluorescence microscopy technique that has attracted a lot of interest in pharmaceutical research during the last decades. The main purpose of FRAP is to measure diffusion on a micrometer scale in a non-invasive and highly specific way, making it capable of measurements in complicated biomaterials, even in vivo. This has proven to be very useful in the investigation of drug diffusion inside different tissues of the body and in materials for controlled drug delivery. FRAP has even found applications for the improvement of several medical therapies and in the field of diagnostics. In this review, an overview is given of the different applications of FRAP in pharmaceutical research, together with essential guidelines on how to perform and analyse FRAP experiments.
Keywords
FRAP, microscopy, drug delivery, diffusion, FLUORESCENCE PHOTOBLEACHING RECOVERY, CULTURED MUSCLE-CELLS, HUMAN CERVICAL-MUCUS, IN-VIVO MEASUREMENT, BRAIN EXTRACELLULAR-SPACE, JUNCTIONAL INTERCELLULAR COMMUNICATION, 3-DIMENSIONAL DIFFUSION MEASUREMENTS, LACUNAR-CANALICULAR SYSTEM, LASER-SCANNING MICROSCOPE, SPATIAL FOURIER-ANALYSIS

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MLA
Deschout, Hendrik, et al. “FRAP in Pharmaceutical Research: Practical Guidelines and Applications in Drug Delivery.” PHARMACEUTICAL RESEARCH, vol. 31, no. 2, 2014, pp. 255–70, doi:10.1007/s11095-013-1146-9.
APA
Deschout, H., Raemdonck, K., Demeester, J., De Smedt, S., & Braeckmans, K. (2014). FRAP in pharmaceutical research: practical guidelines and applications in drug delivery. PHARMACEUTICAL RESEARCH, 31(2), 255–270. https://doi.org/10.1007/s11095-013-1146-9
Chicago author-date
Deschout, Hendrik, Koen Raemdonck, Jo Demeester, Stefaan De Smedt, and Kevin Braeckmans. 2014. “FRAP in Pharmaceutical Research: Practical Guidelines and Applications in Drug Delivery.” PHARMACEUTICAL RESEARCH 31 (2): 255–70. https://doi.org/10.1007/s11095-013-1146-9.
Chicago author-date (all authors)
Deschout, Hendrik, Koen Raemdonck, Jo Demeester, Stefaan De Smedt, and Kevin Braeckmans. 2014. “FRAP in Pharmaceutical Research: Practical Guidelines and Applications in Drug Delivery.” PHARMACEUTICAL RESEARCH 31 (2): 255–270. doi:10.1007/s11095-013-1146-9.
Vancouver
1.
Deschout H, Raemdonck K, Demeester J, De Smedt S, Braeckmans K. FRAP in pharmaceutical research: practical guidelines and applications in drug delivery. PHARMACEUTICAL RESEARCH. 2014;31(2):255–70.
IEEE
[1]
H. Deschout, K. Raemdonck, J. Demeester, S. De Smedt, and K. Braeckmans, “FRAP in pharmaceutical research: practical guidelines and applications in drug delivery,” PHARMACEUTICAL RESEARCH, vol. 31, no. 2, pp. 255–270, 2014.
@article{4392599,
  abstract     = {{Fluorescence recovery after photobleaching (FRAP) is a fluorescence microscopy technique that has attracted a lot of interest in pharmaceutical research during the last decades. The main purpose of FRAP is to measure diffusion on a micrometer scale in a non-invasive and highly specific way, making it capable of measurements in complicated biomaterials, even in vivo. This has proven to be very useful in the investigation of drug diffusion inside different tissues of the body and in materials for controlled drug delivery. FRAP has even found applications for the improvement of several medical therapies and in the field of diagnostics. In this review, an overview is given of the different applications of FRAP in pharmaceutical research, together with essential guidelines on how to perform and analyse FRAP experiments.}},
  author       = {{Deschout, Hendrik and Raemdonck, Koen and Demeester, Jo and De Smedt, Stefaan and Braeckmans, Kevin}},
  issn         = {{0724-8741}},
  journal      = {{PHARMACEUTICAL RESEARCH}},
  keywords     = {{FRAP,microscopy,drug delivery,diffusion,FLUORESCENCE PHOTOBLEACHING RECOVERY,CULTURED MUSCLE-CELLS,HUMAN CERVICAL-MUCUS,IN-VIVO MEASUREMENT,BRAIN EXTRACELLULAR-SPACE,JUNCTIONAL INTERCELLULAR COMMUNICATION,3-DIMENSIONAL DIFFUSION MEASUREMENTS,LACUNAR-CANALICULAR SYSTEM,LASER-SCANNING MICROSCOPE,SPATIAL FOURIER-ANALYSIS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{255--270}},
  title        = {{FRAP in pharmaceutical research: practical guidelines and applications in drug delivery}},
  url          = {{http://doi.org/10.1007/s11095-013-1146-9}},
  volume       = {{31}},
  year         = {{2014}},
}

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