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Self-calibrated concentration measurements of polydisperse nanoparticles

(2013) JOURNAL OF MICROSCOPY. 252(1). p.79-88
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Quantitative characterization of nanoparticles, e.g. accurate estimation of concentration distributions, is critical to many pharmaceutical and biological applications. We present a method that enables for the first time highly accurate size and absolute concentration measurements of polydisperse nanoparticles in solution, based on fluorescence single particle tracking, that are self-calibrated in the sense that the detection region volume is estimated based on the tracking data. The method is evaluated using simulations and experimental data of polystyrene nanospheres in water/sucrose solution. In addition, the method is used to quantify aggregation and clearance of different types of liposomes after intravenous injection in rats, where additional and more accurate information can be obtained that was previously unavailable, which can help elucidate their usefulness as drug carriers.
Keywords
CELLS, FLUORESCENCE, Concentration, SINGLE-PARTICLE TRACKING, BIOLOGICAL-FLUIDS, QUANTUM DOTS, fluorescence single particle tracking, polydispersity, liposomal drug carriers, nanoparticles, size distribution

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Citation

Please use this url to cite or link to this publication:

MLA
Röding, M, Hendrik Deschout, Kevin Braeckmans, et al. “Self-calibrated Concentration Measurements of Polydisperse Nanoparticles.” JOURNAL OF MICROSCOPY 252.1 (2013): 79–88. Print.
APA
Röding, M, Deschout, H., Braeckmans, K., Särkkä, A., & Rudemo, M. (2013). Self-calibrated concentration measurements of polydisperse nanoparticles. JOURNAL OF MICROSCOPY, 252(1), 79–88.
Chicago author-date
Röding, M, Hendrik Deschout, Kevin Braeckmans, A Särkkä, and M Rudemo. 2013. “Self-calibrated Concentration Measurements of Polydisperse Nanoparticles.” Journal of Microscopy 252 (1): 79–88.
Chicago author-date (all authors)
Röding, M, Hendrik Deschout, Kevin Braeckmans, A Särkkä, and M Rudemo. 2013. “Self-calibrated Concentration Measurements of Polydisperse Nanoparticles.” Journal of Microscopy 252 (1): 79–88.
Vancouver
1.
Röding M, Deschout H, Braeckmans K, Särkkä A, Rudemo M. Self-calibrated concentration measurements of polydisperse nanoparticles. JOURNAL OF MICROSCOPY. 2013;252(1):79–88.
IEEE
[1]
M. Röding, H. Deschout, K. Braeckmans, A. Särkkä, and M. Rudemo, “Self-calibrated concentration measurements of polydisperse nanoparticles,” JOURNAL OF MICROSCOPY, vol. 252, no. 1, pp. 79–88, 2013.
@article{4390570,
  abstract     = {Quantitative characterization of nanoparticles, e.g. accurate estimation of concentration distributions, is critical to many pharmaceutical and biological applications. We present a method that enables for the first time highly accurate size and absolute concentration measurements of polydisperse nanoparticles in solution, based on fluorescence single particle tracking, that are self-calibrated in the sense that the detection region volume is estimated based on the tracking data. The method is evaluated using simulations and experimental data of polystyrene nanospheres in water/sucrose solution. In addition, the method is used to quantify aggregation and clearance of different types of liposomes after intravenous injection in rats, where additional and more accurate information can be obtained that was previously unavailable, which can help elucidate their usefulness as drug carriers.},
  author       = {Röding, M and Deschout, Hendrik and Braeckmans, Kevin and Särkkä, A and Rudemo, M},
  issn         = {0022-2720},
  journal      = {JOURNAL OF MICROSCOPY},
  keywords     = {CELLS,FLUORESCENCE,Concentration,SINGLE-PARTICLE TRACKING,BIOLOGICAL-FLUIDS,QUANTUM DOTS,fluorescence single particle tracking,polydispersity,liposomal drug carriers,nanoparticles,size distribution},
  language     = {eng},
  number       = {1},
  pages        = {79--88},
  title        = {Self-calibrated concentration measurements of polydisperse nanoparticles},
  url          = {http://dx.doi.org/10.1111/jmi.12073},
  volume       = {252},
  year         = {2013},
}

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