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ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development

(2014) PLANT CELL. 26(1). p.210-229
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Organization
Project
Biotechnology for a sustainable economy (Bio-Economy)
Project
Bioinformatics: from nucleotids to networks (N2N)
Abstract
The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.
Keywords
CELL-PROLIFERATION, CYTOKININ RESPONSES, DEVELOPING LEAVES, CORE SUBUNIT, CROSS-TALK, ORGAN SIZE, TANDEM AFFINITY PURIFICATION, REPRODUCTIVE-TRACT DEVELOPMENT, GENE-EXPRESSION, GENOME-WIDE ANALYSIS

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Citation

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Chicago
Vercruyssen, Liesbeth, Aurine Verkest, Nathalie Gonzalez Sanchez, Ken Heyndrickx, Dominique Eeckhout, Soon-Ki Han, Teddy Jégu, et al. 2014. “ANGUSTIFOLIA3 Binds to SWI/SNF Chromatin Remodeling Complexes to Regulate Transcription During Arabidopsis Leaf Development.” Plant Cell 26 (1): 210–229.
APA
Vercruyssen, L., Verkest, A., Gonzalez Sanchez, N., Heyndrickx, K., Eeckhout, D., Han, S.-K., Jégu, T., et al. (2014). ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development. PLANT CELL, 26(1), 210–229.
Vancouver
1.
Vercruyssen L, Verkest A, Gonzalez Sanchez N, Heyndrickx K, Eeckhout D, Han S-K, et al. ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development. PLANT CELL. 2014;26(1):210–29.
MLA
Vercruyssen, Liesbeth, Aurine Verkest, Nathalie Gonzalez Sanchez, et al. “ANGUSTIFOLIA3 Binds to SWI/SNF Chromatin Remodeling Complexes to Regulate Transcription During Arabidopsis Leaf Development.” PLANT CELL 26.1 (2014): 210–229. Print.
@article{4388283,
  abstract     = {The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.},
  author       = {Vercruyssen, Liesbeth and Verkest, Aurine and Gonzalez Sanchez, Nathalie and Heyndrickx, Ken and Eeckhout, Dominique and Han, Soon-Ki and J{\'e}gu, Teddy and Archacki, Rafal and Van Leene, Jelle and Andriankaja, Megan and De Bodt, Stefanie and Abeel, Thomas and Coppens, Frederik and Dhondt, Stijn and De Milde, Liesbeth and Vermeersch, Mattias and Maleux, Katrien and Gevaert, Kris and Jerzmanowski, Andrzej and Benhamed, Moussa and Wagner, Doris and Vandepoele, Klaas and De Jaeger, Geert and Inz{\'e}, Dirk},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {CELL-PROLIFERATION,CYTOKININ RESPONSES,DEVELOPING LEAVES,CORE SUBUNIT,CROSS-TALK,ORGAN SIZE,TANDEM AFFINITY PURIFICATION,REPRODUCTIVE-TRACT DEVELOPMENT,GENE-EXPRESSION,GENOME-WIDE ANALYSIS},
  language     = {eng},
  number       = {1},
  pages        = {210--229},
  title        = {ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development},
  url          = {http://dx.doi.org/10.1105/tpc.113.115907},
  volume       = {26},
  year         = {2014},
}

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