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ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development

Liesbeth Vercruyssen, Aurine Verkest, Nathalie Gonzalez Sanchez, Ken Heyndrickx, Dominique Eeckhout UGent, Soon-Ki Han, Teddy Jégu, Rafal Archacki, Jelle Van Leene UGent, Megan Andriankaja, et al. (2014) PLANT CELL. 26(1). p.210-229
abstract
The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CELL-PROLIFERATION, CYTOKININ RESPONSES, DEVELOPING LEAVES, CORE SUBUNIT, CROSS-TALK, ORGAN SIZE, TANDEM AFFINITY PURIFICATION, REPRODUCTIVE-TRACT DEVELOPMENT, GENE-EXPRESSION, GENOME-WIDE ANALYSIS
journal title
PLANT CELL
Plant Cell
volume
26
issue
1
pages
210 - 229
Web of Science type
Article
Web of Science id
000332223200018
JCR category
PLANT SCIENCES
JCR impact factor
9.338 (2014)
JCR rank
4/204 (2014)
JCR quartile
1 (2014)
ISSN
1040-4651
DOI
10.1105/tpc.113.115907
project
Biotechnology for a sustainable economy (Bio-Economy)
project
Bioinformatics: from nucleotids to networks (N2N)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4388283
handle
http://hdl.handle.net/1854/LU-4388283
date created
2014-05-19 13:15:52
date last changed
2016-12-19 15:45:41
@article{4388283,
  abstract     = {The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed the presence of AN3 at the loci of GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1), and ARABIDOPSIS RESPONSE REGULATOR4 (ARR4). Tandem affinity purification of protein complexes using AN3 as bait identified plant SWITCH/SUCROSE NONFERMENTING (SWI/SNF) chromatin remodeling complexes formed around the ATPases BRAHMA (BRM) or SPLAYED. Moreover, SWI/SNF ASSOCIATED PROTEIN 73B (SWP73B) is recruited by AN3 to the promoters of GRF5, GRF3, COL5, and ARR4, and both SWP73B and BRM occupy the HEC1 promoter. Furthermore, we show that AN3 and BRM genetically interact. The data indicate that AN3 associates with chromatin remodelers to regulate transcription. In addition, modification of SWI3C expression levels increases leaf size, underlining the importance of chromatin dynamics for growth regulation. Our results place the SWI/SNF-AN3 module as a major player at the transition from cell proliferation to cell differentiation in a developing leaf.},
  author       = {Vercruyssen, Liesbeth and Verkest, Aurine and Gonzalez Sanchez, Nathalie and Heyndrickx, Ken and Eeckhout, Dominique and Han, Soon-Ki and J{\'e}gu, Teddy and Archacki, Rafal and Van Leene, Jelle and Andriankaja, Megan and De Bodt, Stefanie and Abeel, Thomas and Coppens, Frederik and Dhondt, Stijn and De Milde, Liesbeth and Vermeersch, Mattias and Maleux, Katrien and Gevaert, Kris and Jerzmanowski, Andrzej and Benhamed, Moussa and Wagner, Doris and Vandepoele, Klaas and De Jaeger, Geert and Inz{\'e}, Dirk},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  keyword      = {CELL-PROLIFERATION,CYTOKININ RESPONSES,DEVELOPING LEAVES,CORE SUBUNIT,CROSS-TALK,ORGAN SIZE,TANDEM AFFINITY PURIFICATION,REPRODUCTIVE-TRACT DEVELOPMENT,GENE-EXPRESSION,GENOME-WIDE ANALYSIS},
  language     = {eng},
  number       = {1},
  pages        = {210--229},
  title        = {ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development},
  url          = {http://dx.doi.org/10.1105/tpc.113.115907},
  volume       = {26},
  year         = {2014},
}

Chicago
Vercruyssen, Liesbeth, Aurine Verkest, Nathalie Gonzalez Sanchez, Ken Heyndrickx, Dominique Eeckhout, Soon-Ki Han, Teddy Jégu, et al. 2014. “ANGUSTIFOLIA3 Binds to SWI/SNF Chromatin Remodeling Complexes to Regulate Transcription During Arabidopsis Leaf Development.” Plant Cell 26 (1): 210–229.
APA
Vercruyssen, L., Verkest, A., Gonzalez Sanchez, N., Heyndrickx, K., Eeckhout, D., Han, S.-K., Jégu, T., et al. (2014). ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development. PLANT CELL, 26(1), 210–229.
Vancouver
1.
Vercruyssen L, Verkest A, Gonzalez Sanchez N, Heyndrickx K, Eeckhout D, Han S-K, et al. ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development. PLANT CELL. 2014;26(1):210–29.
MLA
Vercruyssen, Liesbeth, Aurine Verkest, Nathalie Gonzalez Sanchez, et al. “ANGUSTIFOLIA3 Binds to SWI/SNF Chromatin Remodeling Complexes to Regulate Transcription During Arabidopsis Leaf Development.” PLANT CELL 26.1 (2014): 210–229. Print.