Advanced search
1 file | 4.87 MB

Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity

Robin Piron (UGent) , Stefaan De Koker (UGent) , Annelies De Paepe (UGent) , Julie Goossens, Johan Grooten (UGent) , Hans Nauwynck (UGent) and Anna Depicker (UGent)
(2014) PLOS ONE. 9(3).
Author
Organization
Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a disease of swine, caused by an arterivirus, the PRRS virus (PRRSV). This virus infects pigs worldwide and causes huge economic losses. Due to genetic drift, current vaccines are losing their power. Adaptable vaccines could provide a solution to this problem. This study aims at producing in planta a set of antigens derived from the PRRSV glycoproteins (GPs) to be included in a subunit vaccine. We selected the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed platform by removing transmembrane domains (Tm) and/or adding stabilizing protein domains, such as the green fluorescent protein (GFP) and immunoglobulin (IgG) 'Fragment crystallizable' (Fc) chains. Accumulation of the GPs with and without Tm was low, reaching no more than 0.10% of total soluble protein (TSP) in homozygous seed. However, addition of stabilizing domains boosted accumulation up to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also the Fc chains of the porcine IgG3 and murine IgG2a increased antigen accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc chain did not. Antigens with Tm were less susceptible to these manipulations to increase yield. All antigens were produced in the endoplasmic reticulum and accordingly, they carried high-mannose N-glycans. The immunogenicity of several of those antigens was assessed and we show that vaccination with purified antigens did elicit the production of antibodies with virus neutralizing activity in mice but not in pigs.
Keywords
SUBUNIT VACCINE, DENDRITIC CELLS, ARABIDOPSIS SEEDS, EPIDEMIC ABORTION, NEUTRALIZING ANTIBODIES, FUSION PROTEINS, N-GLYCOSYLATION PATTERN, MYSTERY SWINE DISEASE, PROTEIN-PRODUCTION, LELYSTAD-VIRUS

Downloads

  • Piron et al. 2014 PLoS ONE 9 e91386.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 4.87 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Piron, Robin, Stefaan De Koker, Annelies De Paepe, Julie Goossens, Johan Grooten, Hans Nauwynck, and Anna Depicker. 2014. “Boosting in Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity.” Plos One 9 (3).
APA
Piron, R., De Koker, S., De Paepe, A., Goossens, J., Grooten, J., Nauwynck, H., & Depicker, A. (2014). Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity. PLOS ONE, 9(3).
Vancouver
1.
Piron R, De Koker S, De Paepe A, Goossens J, Grooten J, Nauwynck H, et al. Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity. PLOS ONE. 2014;9(3).
MLA
Piron, Robin, Stefaan De Koker, Annelies De Paepe, et al. “Boosting in Planta Production of Antigens Derived from the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Subsequent Evaluation of Their Immunogenicity.” PLOS ONE 9.3 (2014): n. pag. Print.
@article{4388244,
  abstract     = {Porcine reproductive and respiratory syndrome (PRRS) is a disease of swine, caused by an arterivirus, the PRRS virus (PRRSV). This virus infects pigs worldwide and causes huge economic losses. Due to genetic drift, current vaccines are losing their power. Adaptable vaccines could provide a solution to this problem. This study aims at producing in planta a set of antigens derived from the PRRSV glycoproteins (GPs) to be included in a subunit vaccine. We selected the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed platform by removing transmembrane domains (Tm) and/or adding stabilizing protein domains, such as the green fluorescent protein (GFP) and immunoglobulin (IgG) 'Fragment crystallizable' (Fc) chains. Accumulation of the GPs with and without Tm was low, reaching no more than 0.10% of total soluble protein (TSP) in homozygous seed. However, addition of stabilizing domains boosted accumulation up to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also the Fc chains of the porcine IgG3 and murine IgG2a increased antigen accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc chain did not. Antigens with Tm were less susceptible to these manipulations to increase yield. All antigens were produced in the endoplasmic reticulum and accordingly, they carried high-mannose N-glycans. The immunogenicity of several of those antigens was assessed and we show that vaccination with purified antigens did elicit the production of antibodies with virus neutralizing activity in mice but not in pigs.},
  articleno    = {e91386},
  author       = {Piron, Robin and De Koker, Stefaan and De Paepe, Annelies and Goossens, Julie and Grooten, Johan and Nauwynck, Hans and Depicker, Anna},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {SUBUNIT VACCINE,DENDRITIC CELLS,ARABIDOPSIS SEEDS,EPIDEMIC ABORTION,NEUTRALIZING ANTIBODIES,FUSION PROTEINS,N-GLYCOSYLATION PATTERN,MYSTERY SWINE DISEASE,PROTEIN-PRODUCTION,LELYSTAD-VIRUS},
  language     = {eng},
  number       = {3},
  pages        = {16},
  title        = {Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity},
  url          = {http://dx.doi.org/10.1371/journal.pone.0091386},
  volume       = {9},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: