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Improved gas chromatography-flame ionisation detector analytical method for the analysis of epoxy fatty acids

Edward Mubiru (UGent) , Kshitij Shrestha (UGent) , Antonios Papastergiadis (UGent) and Bruno De Meulenaer (UGent)
(2013) AOCS, 105th Annual meeting, Abstrats.
Author
Organization
Abstract
In this study an improved method for analysis of epoxy fatty acids is reported. Data obtained from analysis of polar fatty acids has previously been presented, but due to the high number of compounds that co-elute in the polar fraction, the resultant chromatograms are complex which may lead to compromising the accuracy of the data. A three steps separation of fatty acid methyl esters (FAMEs) by solid-phase extraction (SPE) on a silica gel column to remove hydroxy fatty acid interferences was proposed. This approach is opposed to a two step separation procedure that has been often used to prevent analytical interferences caused by non-altered fatty acids. A gas chromatograph with a flame ionisation detector (GC-FID) equipped with a polar CP-Sil 88™ column was used. Quantification was based on the use of methyl nonadecanoate (C19:0), as an internal standard. Individual mono epoxy fatty acids were well separated without co-eluting compounds. The optimised method was finally applied to screen epoxy fatty acids in 37 fresh oil samples. Results obtained for the total epoxy fatty acids were in the range 0.03 – 2 mg g-1 of oil with repeatability coefficient of variation (CV) ranging from 2.8 to 9.9 % for duplicate analysis showing that the results obtained are repeatable
Keywords
epoxy fatty acids, Lipid oxidation, oxygenated polar fatty acids, FAMEs, GC-FID

Citation

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MLA
Mubiru, Edward, et al. “Improved Gas Chromatography-Flame Ionisation Detector Analytical Method for the Analysis of Epoxy Fatty Acids.” AOCS, 105th Annual Meeting, Abstrats, American Oil Chemists’ Society (AOCS), 2013.
APA
Mubiru, E., Shrestha, K., Papastergiadis, A., & De Meulenaer, B. (2013). Improved gas chromatography-flame ionisation detector analytical method for the analysis of epoxy fatty acids. AOCS, 105th Annual Meeting, Abstrats. Presented at the 105th AOCS Annual meeting and Expo, San Antonio, Texas, USA.
Chicago author-date
Mubiru, Edward, Kshitij Shrestha, Antonios Papastergiadis, and Bruno De Meulenaer. 2013. “Improved Gas Chromatography-Flame Ionisation Detector Analytical Method for the Analysis of Epoxy Fatty Acids.” In AOCS, 105th Annual Meeting, Abstrats. American Oil Chemists’ Society (AOCS).
Chicago author-date (all authors)
Mubiru, Edward, Kshitij Shrestha, Antonios Papastergiadis, and Bruno De Meulenaer. 2013. “Improved Gas Chromatography-Flame Ionisation Detector Analytical Method for the Analysis of Epoxy Fatty Acids.” In AOCS, 105th Annual Meeting, Abstrats. American Oil Chemists’ Society (AOCS).
Vancouver
1.
Mubiru E, Shrestha K, Papastergiadis A, De Meulenaer B. Improved gas chromatography-flame ionisation detector analytical method for the analysis of epoxy fatty acids. In: AOCS, 105th Annual meeting, Abstrats. American Oil Chemists’ Society (AOCS); 2013.
IEEE
[1]
E. Mubiru, K. Shrestha, A. Papastergiadis, and B. De Meulenaer, “Improved gas chromatography-flame ionisation detector analytical method for the analysis of epoxy fatty acids,” in AOCS, 105th Annual meeting, Abstrats, San Antonio, Texas, USA, 2013.
@inproceedings{4387819,
  abstract     = {{In this study an improved method for analysis of epoxy fatty acids is reported. Data obtained from analysis of polar fatty acids has previously been presented, but due to the high number of compounds that co-elute in the polar fraction, the resultant chromatograms are complex which may lead to compromising the accuracy of the data. A three steps separation of fatty acid methyl esters (FAMEs) by solid-phase extraction (SPE) on a silica gel column to remove hydroxy fatty acid interferences was proposed. This approach is opposed to a two step separation procedure that has been often used to prevent analytical interferences caused by non-altered fatty acids. A gas chromatograph with a flame ionisation detector (GC-FID) equipped with a polar CP-Sil 88™ column was used. Quantification was based on the use of methyl nonadecanoate (C19:0), as an internal standard. Individual mono epoxy fatty acids were well separated without co-eluting compounds. The optimised method was finally applied to screen epoxy fatty acids in 37 fresh oil samples. Results obtained for the total epoxy fatty acids were in the range 0.03 – 2 mg g-1 of oil with repeatability coefficient of variation (CV) ranging from 2.8 to 9.9 % for duplicate analysis showing that the results obtained are repeatable}},
  author       = {{Mubiru, Edward and Shrestha, Kshitij and Papastergiadis, Antonios and De Meulenaer, Bruno}},
  booktitle    = {{AOCS, 105th Annual meeting, Abstrats}},
  keywords     = {{epoxy fatty acids,Lipid oxidation,oxygenated polar fatty acids,FAMEs,GC-FID}},
  language     = {{eng}},
  location     = {{San Antonio, Texas, USA}},
  publisher    = {{American Oil Chemists' Society (AOCS)}},
  title        = {{Improved gas chromatography-flame ionisation detector analytical method for the analysis of epoxy fatty acids}},
  year         = {{2013}},
}