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Biofilm models for the food industry: hot spots for plasmid transfer?

(2014) PATHOGENS AND DISEASE. 70(3). p.332-338
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Abstract
Biofilms represent a substantial problem in the food industry, with food spoilage, equipment failure, and public health aspects to consider. Besides, biofilms may be a hot spot for plasmid transfer, by which antibiotic resistance can be disseminated to potential foodborne pathogens. This study investigated biomass and plasmid transfer in dual-species (Pseudomonas putida and Escherichia coli) biofilm models relevant to the food industry. Two different configurations (flow-through and drip-flow) and two different inoculation procedures (donor-recipient and recipient-donor) were tested. The drip-flow configuration integrated stainless steel coupons in the setup while the flow-through configuration included a glass flow cell and silicone tubing. The highest biomass density [10 log (cells cm-(2))] was obtained in the silicone tubing when first the recipient strain was inoculated. High plasmid transfer ratios, up to 1/10 (transconjugants/total bacteria), were found. Depending on the order of inoculation, a difference in transfer efficiency between the biofilm models could be found. The ease by which the multiresistance plasmid was transferred highlights the importance of biofilms in the food industry as hot spots for the acquisition of multiresistance plasmids. This can impede the treatment of foodborne illnesses if pathogens acquire this multiresistance in or from the biofilm.
Keywords
conjugation, :dual-species biofilms, antibiotic resistance, ABIOTIC SURFACES, RIBOSOMAL-RNA, ANTIMICROBIAL RESISTANCE, MICROBIAL COMMUNITY, GRADIENT GEL-ELECTROPHORESIS, ESCHERICHIA-COLI BIOFILMS, ANTIBIOTIC-RESISTANCE, ACTIVATED-SLUDGE, SURFACE MATING SYSTEMS, AIR-LIQUID INTERFACE

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Citation

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MLA
Van Meervenne, Eva, et al. “Biofilm Models for the Food Industry: Hot Spots for Plasmid Transfer?” PATHOGENS AND DISEASE, vol. 70, no. 3, 2014, pp. 332–38, doi:10.1111/2049-632X.12134.
APA
Van Meervenne, E., De Weirdt, R., Van Coillie, E., Devlieghere, F., Herman, L., & Boon, N. (2014). Biofilm models for the food industry: hot spots for plasmid transfer? PATHOGENS AND DISEASE, 70(3), 332–338. https://doi.org/10.1111/2049-632X.12134
Chicago author-date
Van Meervenne, Eva, Rosemarie De Weirdt, Els Van Coillie, Frank Devlieghere, Lieve Herman, and Nico Boon. 2014. “Biofilm Models for the Food Industry: Hot Spots for Plasmid Transfer?” PATHOGENS AND DISEASE 70 (3): 332–38. https://doi.org/10.1111/2049-632X.12134.
Chicago author-date (all authors)
Van Meervenne, Eva, Rosemarie De Weirdt, Els Van Coillie, Frank Devlieghere, Lieve Herman, and Nico Boon. 2014. “Biofilm Models for the Food Industry: Hot Spots for Plasmid Transfer?” PATHOGENS AND DISEASE 70 (3): 332–338. doi:10.1111/2049-632X.12134.
Vancouver
1.
Van Meervenne E, De Weirdt R, Van Coillie E, Devlieghere F, Herman L, Boon N. Biofilm models for the food industry: hot spots for plasmid transfer? PATHOGENS AND DISEASE. 2014;70(3):332–8.
IEEE
[1]
E. Van Meervenne, R. De Weirdt, E. Van Coillie, F. Devlieghere, L. Herman, and N. Boon, “Biofilm models for the food industry: hot spots for plasmid transfer?,” PATHOGENS AND DISEASE, vol. 70, no. 3, pp. 332–338, 2014.
@article{4387274,
  abstract     = {{Biofilms represent a substantial problem in the food industry, with food spoilage, equipment failure, and public health aspects to consider. Besides, biofilms may be a hot spot for plasmid transfer, by which antibiotic resistance can be disseminated to potential foodborne pathogens. This study investigated biomass and plasmid transfer in dual-species (Pseudomonas putida and Escherichia coli) biofilm models relevant to the food industry. Two different configurations (flow-through and drip-flow) and two different inoculation procedures (donor-recipient and recipient-donor) were tested. The drip-flow configuration integrated stainless steel coupons in the setup while the flow-through configuration included a glass flow cell and silicone tubing. The highest biomass density [10 log (cells cm-(2))] was obtained in the silicone tubing when first the recipient strain was inoculated. High plasmid transfer ratios, up to 1/10 (transconjugants/total bacteria), were found. Depending on the order of inoculation, a difference in transfer efficiency between the biofilm models could be found. The ease by which the multiresistance plasmid was transferred highlights the importance of biofilms in the food industry as hot spots for the acquisition of multiresistance plasmids. This can impede the treatment of foodborne illnesses if pathogens acquire this multiresistance in or from the biofilm.}},
  author       = {{Van Meervenne, Eva and De Weirdt, Rosemarie and Van Coillie, Els and Devlieghere, Frank and Herman, Lieve and Boon, Nico}},
  issn         = {{2049-632X}},
  journal      = {{PATHOGENS AND DISEASE}},
  keywords     = {{conjugation,:dual-species biofilms,antibiotic resistance,ABIOTIC SURFACES,RIBOSOMAL-RNA,ANTIMICROBIAL RESISTANCE,MICROBIAL COMMUNITY,GRADIENT GEL-ELECTROPHORESIS,ESCHERICHIA-COLI BIOFILMS,ANTIBIOTIC-RESISTANCE,ACTIVATED-SLUDGE,SURFACE MATING SYSTEMS,AIR-LIQUID INTERFACE}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{332--338}},
  title        = {{Biofilm models for the food industry: hot spots for plasmid transfer?}},
  url          = {{http://doi.org/10.1111/2049-632X.12134}},
  volume       = {{70}},
  year         = {{2014}},
}

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