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Cytotoxic activity of Laserpitium latifolium L. extract and its daucane and phenylpropanoid constituents

(2013) RECORDS OF NATURAL PRODUCTS. 7(3). p.245-249
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Abstract
In the search for novel cytotoxic agents, sesquiterpenoids and phenylpropanoids have provided interesting lead compounds. From the chloroform extract of the underground parts of Laserpitium latifolium, the daucane sesquiterpenoids laserpitin and acetyldesoxodehydrolaserpitin, and the phenylpropanoids laserin and latifolon were isolated as the major compounds. Acetyldesoxodehydrolaserpitin is identified for the first time in the genus Laserpitium. Using a MTT and a sulforhodamine B (SRB) assay, the cytotoxic and antiproliferative activity of the extract and compounds laserpitin, acetyldesoxodehydrolaserpitin and laserin was tested in two closely related human breast adenocarcinoma cell lines, ie. the invasive MCF 7/6 and the non-invasive MCF 7/AZ. The IC50 values of the extract were in the range of 184.72 - 397.16 mu g/mL, with the most potent effect observed in the MTT test on the MCF 7/6 line. Among the tested compounds, acetyldesoxodehydrolaserpitin exerted a most potent, concentration-dependent effect (IC50 values of 0.60 and 0.51 mu M in the MCF 7/6 cell line, and 2.29 mu M and 31.87 mu M in the MCF 7/AZ cell line in the MTT and SRB test, respectively). The effect of laserin was more pronounced in the MTT test (IC50 of 4.57 and 2.46 mu M in the MCF 7/6 and MCF 7/AZ, respectively).
Keywords
TERPENES, SESQUITERPENES, ASSAY, Laserpitium latifolium, phenylpropanoids, Daucane esters, cytotoxic activity.

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Citation

Please use this url to cite or link to this publication:

Chicago
Popović, Višnja, Arne Heyerick, Silvana Petrović, Serge Van Calenbergh, Izet Karalic, Marjan Niketić, and Dieter Deforce. 2013. “Cytotoxic Activity of Laserpitium Latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents.” Records of Natural Products 7 (3): 245–249.
APA
Popović, V., Heyerick, A., Petrović, S., Van Calenbergh, S., Karalic, I., Niketić, M., & Deforce, D. (2013). Cytotoxic activity of Laserpitium latifolium L. extract and its daucane and phenylpropanoid constituents. RECORDS OF NATURAL PRODUCTS, 7(3), 245–249.
Vancouver
1.
Popović V, Heyerick A, Petrović S, Van Calenbergh S, Karalic I, Niketić M, et al. Cytotoxic activity of Laserpitium latifolium L. extract and its daucane and phenylpropanoid constituents. RECORDS OF NATURAL PRODUCTS. 2013;7(3):245–9.
MLA
Popović, Višnja, Arne Heyerick, Silvana Petrović, et al. “Cytotoxic Activity of Laserpitium Latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents.” RECORDS OF NATURAL PRODUCTS 7.3 (2013): 245–249. Print.
@article{4375805,
  abstract     = {In the search for novel cytotoxic agents, sesquiterpenoids and phenylpropanoids have provided interesting lead compounds. From the chloroform extract of the underground parts of Laserpitium latifolium, the daucane sesquiterpenoids laserpitin and acetyldesoxodehydrolaserpitin, and the phenylpropanoids laserin and latifolon were isolated as the major compounds. Acetyldesoxodehydrolaserpitin is identified for the first time in the genus Laserpitium. Using a MTT and a sulforhodamine B (SRB) assay, the cytotoxic and antiproliferative activity of the extract and compounds laserpitin, acetyldesoxodehydrolaserpitin and laserin was tested in two closely related human breast adenocarcinoma cell lines, ie. the invasive MCF 7/6 and the non-invasive MCF 7/AZ. The IC50 values of the extract were in the range of 184.72 - 397.16 mu g/mL, with the most potent effect observed in the MTT test on the MCF 7/6 line. Among the tested compounds, acetyldesoxodehydrolaserpitin exerted a most potent, concentration-dependent effect (IC50 values of 0.60 and 0.51 mu M in the MCF 7/6 cell line, and 2.29 mu M and 31.87 mu M in the MCF 7/AZ cell line in the MTT and SRB test, respectively). The effect of laserin was more pronounced in the MTT test (IC50 of 4.57 and 2.46 mu M in the MCF 7/6 and MCF 7/AZ, respectively).},
  author       = {Popovi\'{c}, Vi\v{s}nja and Heyerick, Arne and Petrovi\'{c}, Silvana and Van Calenbergh, Serge and Karalic, Izet and Niketi\'{c}, Marjan and Deforce, Dieter},
  issn         = {1307-6167},
  journal      = {RECORDS OF NATURAL PRODUCTS},
  language     = {eng},
  number       = {3},
  pages        = {245--249},
  title        = {Cytotoxic activity of Laserpitium latifolium L. extract and its daucane and phenylpropanoid constituents},
  url          = {http://www.acgpubs.org/RNP/2013/Volume7/Issue\%201/39-RNP-1210-181.pdf},
  volume       = {7},
  year         = {2013},
}

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