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Soft tissue discrimination with contrast agents using micro-CT scanning

Emilie Descamps (UGent) , Alicja Sochacka (UGent) , Barbara De Kegel (UGent) , Denis Van Loo (UGent) , Luc Van Hoorebeke (UGent) and Dominique Adriaens (UGent)
(2014) BELGIAN JOURNAL OF ZOOLOGY. 144(1). p.20-40
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Abstract
The use of high resolution, three-dimensional visualization has been receiving a growing interest within life sciences, with non-invasive imaging tools becoming more readily accessible. Although initially useful for visualizing mineralized tissues, recent developments are promising for studying soft tissues as well. Especially for microCT scanning, several X-ray contrast enhancers are performant in sufficiently contrasting soft tissue organ systems by a different attenuation strength of X-rays. Overall visualization of soft tissue organs has proven to be possible, although the tissue specific capacities of these enhancers remain unclear. In this study, we tested several contrast agents for their usefulness to discriminate between tissue types and organs, using three model organisms (mouse, zebrafish and Xenopus). Specimens were stained with osmium tetroxide (OsO4), phosphomolybdic acid (PMA) and phosphotungstic acid (PTA), and were scanned using high resolution microtomography. The contrasting potentials between tissue types and organs are described based on volume renderings and virtual sections. In general, PTA and PMA appeared to allow a better discrimination. Especially epithelial structures, cell dense brain regions, liver, lung and blood could be easily distinguished. The PMA yielded the best results, allowing a discrimination even at the level of cell layers. Our results show that those staining techniques combined with microCT imaging have a good potential for future research in life sciences.
Keywords
contrast agents, vertebrates, soft tissue, 3D visualization, micro-CT scanning, RAY COMPUTED-TOMOGRAPHY, ENHANCED MICROCT, OSMIUM-TETROXIDE, HIGH-RESOLUTION, MOUSE, MICROTOMOGRAPHY, VISUALIZATION, ANATOMY, QUANTIFICATION, MORPHOLOGY

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Citation

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MLA
Descamps, Emilie, Alicja Sochacka, Barbara De Kegel, et al. “Soft Tissue Discrimination with Contrast Agents Using micro-CT Scanning.” BELGIAN JOURNAL OF ZOOLOGY 144.1 (2014): 20–40. Print.
APA
Descamps, E., Sochacka, A., De Kegel, B., Van Loo, D., Van Hoorebeke, L., & Adriaens, D. (2014). Soft tissue discrimination with contrast agents using micro-CT scanning. BELGIAN JOURNAL OF ZOOLOGY, 144(1), 20–40.
Chicago author-date
Descamps, Emilie, Alicja Sochacka, Barbara De Kegel, Denis Van Loo, Luc Van Hoorebeke, and Dominique Adriaens. 2014. “Soft Tissue Discrimination with Contrast Agents Using micro-CT Scanning.” Belgian Journal of Zoology 144 (1): 20–40.
Chicago author-date (all authors)
Descamps, Emilie, Alicja Sochacka, Barbara De Kegel, Denis Van Loo, Luc Van Hoorebeke, and Dominique Adriaens. 2014. “Soft Tissue Discrimination with Contrast Agents Using micro-CT Scanning.” Belgian Journal of Zoology 144 (1): 20–40.
Vancouver
1.
Descamps E, Sochacka A, De Kegel B, Van Loo D, Van Hoorebeke L, Adriaens D. Soft tissue discrimination with contrast agents using micro-CT scanning. BELGIAN JOURNAL OF ZOOLOGY. 2014;144(1):20–40.
IEEE
[1]
E. Descamps, A. Sochacka, B. De Kegel, D. Van Loo, L. Van Hoorebeke, and D. Adriaens, “Soft tissue discrimination with contrast agents using micro-CT scanning,” BELGIAN JOURNAL OF ZOOLOGY, vol. 144, no. 1, pp. 20–40, 2014.
@article{4372359,
  abstract     = {The use of high resolution, three-dimensional visualization has been receiving a growing interest within life sciences, with non-invasive imaging tools becoming more readily accessible. Although initially useful for visualizing mineralized tissues, recent developments are promising for studying soft tissues as well. Especially for microCT scanning, several X-ray contrast enhancers are performant in sufficiently contrasting soft tissue organ systems by a different attenuation strength of X-rays. Overall visualization of soft tissue organs has proven to be possible, although the tissue specific capacities of these enhancers remain unclear. In this study, we tested several contrast agents for their usefulness to discriminate between tissue types and organs, using three model organisms (mouse, zebrafish and Xenopus). Specimens were stained with osmium tetroxide (OsO4), phosphomolybdic acid (PMA) and phosphotungstic acid (PTA), and were scanned using high resolution microtomography. The contrasting potentials between tissue types and organs are described based on volume renderings and virtual sections. In general, PTA and PMA appeared to allow a better discrimination. Especially epithelial structures, cell dense brain regions, liver, lung and blood could be easily distinguished. The PMA yielded the best results, allowing a discrimination even at the level of cell layers. Our results show that those staining techniques combined with microCT imaging have a good potential for future research in life sciences.},
  author       = {Descamps, Emilie and Sochacka, Alicja and De Kegel, Barbara and Van Loo, Denis and Van Hoorebeke, Luc and Adriaens, Dominique},
  issn         = {0777-6276},
  journal      = {BELGIAN JOURNAL OF ZOOLOGY},
  keywords     = {contrast agents,vertebrates,soft tissue,3D visualization,micro-CT scanning,RAY COMPUTED-TOMOGRAPHY,ENHANCED MICROCT,OSMIUM-TETROXIDE,HIGH-RESOLUTION,MOUSE,MICROTOMOGRAPHY,VISUALIZATION,ANATOMY,QUANTIFICATION,MORPHOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {20--40},
  title        = {Soft tissue discrimination with contrast agents using micro-CT scanning},
  volume       = {144},
  year         = {2014},
}

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