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Sustained release and activation of the growth factor basic fibroblast growth factor from loaded scaffolds in heart valve tissue engineering

Pamela Somers (UGent) , RAJKISHAN NARINE (UGent) , Filip De Somer (UGent) , Filip De Vos (UGent) and Guido Van Nooten (UGent)
(2008) GROWTH FACTORS. 26(5). p.293-299
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Abstract
Objectives. Loading of biological matrices offers an opportunity to induce specific cell behaviour. We previously reported the use of growth factors to promote cell invasion and proliferation in tissue valve engineering. We investigated biological matrices preloaded with heparin as an ionically attractive template for the binding, activation and sustained release of basic fibroblast growth factor (bFGF). Methods. Heparin loading concentrations were evaluated and different incubation times were tested. Heparin and heparin-bound bFGF uptake and release were evaluated by I-123 radio-labelling. Biological activity of bFGF was evaluated in vitro. Results. Maximum heparin uptake was observed for 2000 mu g/ml at 2h and stabilized thereafter. bFGF-loaded matrices showed an initial burst release of 15% within 4h and thereafter sustained release reaching 21% at 24h. Released bFGF was bioactive. Conclusions. This model would be useful in tissue engineering using porcine aortic matrices and could be applied using other growth factors or combinations.

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Chicago
Somers, Pamela, RAJKISHAN NARINE, Filip De Somer, Filip De Vos, and Guido Van Nooten. 2008. “Sustained Release and Activation of the Growth Factor Basic Fibroblast Growth Factor from Loaded Scaffolds in Heart Valve Tissue Engineering.” Growth Factors 26 (5): 293–299.
APA
Somers, P., NARINE, R., De Somer, F., De Vos, F., & Van Nooten, G. (2008). Sustained release and activation of the growth factor basic fibroblast growth factor from loaded scaffolds in heart valve tissue engineering. GROWTH FACTORS, 26(5), 293–299.
Vancouver
1.
Somers P, NARINE R, De Somer F, De Vos F, Van Nooten G. Sustained release and activation of the growth factor basic fibroblast growth factor from loaded scaffolds in heart valve tissue engineering. GROWTH FACTORS. 2008;26(5):293–9.
MLA
Somers, Pamela, RAJKISHAN NARINE, Filip De Somer, et al. “Sustained Release and Activation of the Growth Factor Basic Fibroblast Growth Factor from Loaded Scaffolds in Heart Valve Tissue Engineering.” GROWTH FACTORS 26.5 (2008): 293–299. Print.
@article{437235,
  abstract     = {Objectives. Loading of biological matrices offers an opportunity to induce specific cell behaviour. We previously reported the use of growth factors to promote cell invasion and proliferation in tissue valve engineering.

We investigated biological matrices preloaded with heparin as an ionically attractive template for the binding, activation and sustained release of basic fibroblast growth factor (bFGF).

Methods. Heparin loading concentrations were evaluated and different incubation times were tested. Heparin and heparin-bound bFGF uptake and release were evaluated by I-123 radio-labelling. Biological activity of bFGF was evaluated in vitro.

Results. Maximum heparin uptake was observed for 2000 mu g/ml at 2h and stabilized thereafter. bFGF-loaded matrices showed an initial burst release of 15% within 4h and thereafter sustained release reaching 21% at 24h. Released bFGF was bioactive.

Conclusions. This model would be useful in tissue engineering using porcine aortic matrices and could be applied using other growth factors or combinations.},
  author       = {Somers, Pamela and NARINE, RAJKISHAN and De Somer, Filip and De Vos, Filip and Van Nooten, Guido},
  issn         = {0897-7194},
  journal      = {GROWTH FACTORS},
  language     = {eng},
  number       = {5},
  pages        = {293--299},
  title        = {Sustained release and activation of the growth factor basic fibroblast growth factor from loaded scaffolds in heart valve tissue engineering},
  url          = {http://dx.doi.org/10.1080/08977190802303645},
  volume       = {26},
  year         = {2008},
}

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