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CE analysis of γ-globulin mobility and potential clinical utility

Dieter Vanderschaeghe, Evi Debruyne UGent, Hans Van Vlierberghe UGent, Nico Callewaert UGent and Joris Delanghe UGent (2013) Clinical applications of capillary electrophoresis : methods and protocols. In Methods in Molecular Biology 919. p.249-257
abstract
Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction. Moreover, we found that the migration time of the g-globulin fraction can be reproducibly determined (CV = 1.1%). Immunoglobulins were purified from serum using protein l-agarose and their N-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this moderately correlates with the migration time of the g-globulins (r = 0.2– 0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy (area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical test.
Please use this url to cite or link to this publication:
author
organization
alternative title
CE analysis of gamma-globulin mobility and potential clinical utility
year
type
bookChapter
publication status
published
subject
book title
Clinical applications of capillary electrophoresis : methods and protocols
editor
Terry M Phillips and Heather Kalish
series title
Methods in Molecular Biology
volume
919
pages
249 - 257
publisher
Humana Press
place of publication
New York, NY, USA
ISSN
1064-3745
ISBN
9781627030298
9781627030281
DOI
10.1007/978-1-62703-029-8_22
language
English
UGent publication?
yes
classification
B2
copyright statement
I have transferred the copyright for this publication to the publisher
id
4364794
handle
http://hdl.handle.net/1854/LU-4364794
date created
2014-04-16 17:17:17
date last changed
2017-01-02 09:54:09
@incollection{4364794,
  abstract     = {Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction. Moreover, we found that the migration time of the g-globulin fraction can be reproducibly determined (CV = 1.1\%). Immunoglobulins were purified from serum using protein l-agarose and their N-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this moderately correlates with the migration time of the g-globulins (r = 0.2-- 0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy (area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical test.},
  author       = {Vanderschaeghe, Dieter and Debruyne, Evi and Van Vlierberghe, Hans and Callewaert, Nico and Delanghe, Joris},
  booktitle    = {Clinical applications of capillary electrophoresis : methods and protocols},
  editor       = {Phillips, Terry M and Kalish, Heather},
  isbn         = {9781627030298},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {249--257},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {CE analysis of \ensuremath{\gamma}-globulin mobility and potential clinical utility},
  url          = {http://dx.doi.org/10.1007/978-1-62703-029-8\_22},
  volume       = {919},
  year         = {2013},
}

Chicago
Vanderschaeghe, Dieter, Evi Debruyne, Hans Van Vlierberghe, Nico Callewaert, and Joris Delanghe. 2013. “CE Analysis of Γ-globulin Mobility and Potential Clinical Utility.” In Clinical Applications of Capillary Electrophoresis : Methods and Protocols, ed. Terry M Phillips and Heather Kalish, 919:249–257. New York, NY, USA: Humana Press.
APA
Vanderschaeghe, D., Debruyne, E., Van Vlierberghe, H., Callewaert, N., & Delanghe, J. (2013). CE analysis of γ-globulin mobility and potential clinical utility. In T. M. Phillips & H. Kalish (Eds.), Clinical applications of capillary electrophoresis : methods and protocols (Vol. 919, pp. 249–257). New York, NY, USA: Humana Press.
Vancouver
1.
Vanderschaeghe D, Debruyne E, Van Vlierberghe H, Callewaert N, Delanghe J. CE analysis of γ-globulin mobility and potential clinical utility. In: Phillips TM, Kalish H, editors. Clinical applications of capillary electrophoresis : methods and protocols. New York, NY, USA: Humana Press; 2013. p. 249–57.
MLA
Vanderschaeghe, Dieter, Evi Debruyne, Hans Van Vlierberghe, et al. “CE Analysis of Γ-globulin Mobility and Potential Clinical Utility.” Clinical Applications of Capillary Electrophoresis : Methods and Protocols. Ed. Terry M Phillips & Heather Kalish. Vol. 919. New York, NY, USA: Humana Press, 2013. 249–257. Print.