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Modulation of the hormone setting by Rhodococcus fascians results in ectopic KNOX activation in Arabidopsis

Stephen Depuydt UGent, Karel Doležal, Maria Van Lijsebettens UGent, Thomas Moritz, Marcella Holsters UGent and Danny Vereecke UGent (2008) PLANT PHYSIOLOGY. 146(3). p.1267-1281
abstract
The biotrophic actinomycete Rhodococcus fascians has a profound impact on plant development and a common aspect of the symptomatology is the deformation of infected leaves. In Arabidopsis ( Arabidopsis thaliana), the serrated leaf margins formed upon infection resemble the leaf phenotype of transgenic plants with ectopic expression of KNOTTED-like homeobox (KNOX) genes. Through transcript profiling, we demonstrate that class-I KNOX genes are transcribed in symptomatic leaves. Functional analysis revealed that BREVIPEDICELLUS/KNOTTED-LIKE1 and mainly SHOOT MERISTEMLESS were essential for the observed leaf dissection. However, these results also positioned the KNOX genes downstream in the signaling cascade triggered by R. fascians infection. The much faster activation of ARABIDOPSIS RESPONSE REGULATOR5 and the establishment of homeostatic and feedback mechanisms to control cytokinin (CK) levels support the overrepresentation of this hormone in infected plants due to the secretion by the pathogen, thereby placing the CK response high up in the cascade. Hormone measurements show a net decrease of tested CKs, indicating either that secretion by the bacterium and degradation by the plant are in balance, or, as suggested by the strong reaction of 35S:CKX plants, that other CKs are at play. At early time points of the interaction, activation of gibberellin 2-oxidase presumably installs a local hormonal setting favorable for meristematic activity that provokes leaf serrations. The results are discussed in the context of symptom development, evasion of plant defense, and the establishment of a specific niche by R. fascians.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CYTOKININ BIOSYNTHESIS, ASYMMETRIC LEAVES1, KNOTTED1-LIKE HOMEOBOX GENE, MERISTEM ACTIVITY, LEAF DEVELOPMENT, CORYNEBACTERIUM-FASCIANS, SHOOT MERISTEMLESS, GIBBERELLIN METABOLISM, PLANT DEVELOPMENT, CELL-DIVISION
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
146
issue
3
pages
1267 - 1281
Web of Science type
Article
Web of Science id
000256412200049
JCR category
PLANT SCIENCES
JCR impact factor
6.11 (2008)
JCR rank
12/155 (2008)
JCR quartile
1 (2008)
ISSN
0032-0889
DOI
10.1104/pp.107.113969
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
436432
handle
http://hdl.handle.net/1854/LU-436432
date created
2008-11-04 19:59:00
date last changed
2016-12-19 15:44:24
@article{436432,
  abstract     = {The biotrophic actinomycete Rhodococcus fascians has a profound impact on plant development and a common aspect of the symptomatology is the deformation of infected leaves. In Arabidopsis ( Arabidopsis thaliana), the serrated leaf margins formed upon infection resemble the leaf phenotype of transgenic plants with ectopic expression of KNOTTED-like homeobox (KNOX) genes. Through transcript profiling, we demonstrate that class-I KNOX genes are transcribed in symptomatic leaves. Functional analysis revealed that BREVIPEDICELLUS/KNOTTED-LIKE1 and mainly SHOOT MERISTEMLESS were essential for the observed leaf dissection. However, these results also positioned the KNOX genes downstream in the signaling cascade triggered by R. fascians infection. The much faster activation of ARABIDOPSIS RESPONSE REGULATOR5 and the establishment of homeostatic and feedback mechanisms to control cytokinin (CK) levels support the overrepresentation of this hormone in infected plants due to the secretion by the pathogen, thereby placing the CK response high up in the cascade. Hormone measurements show a net decrease of tested CKs, indicating either that secretion by the bacterium and degradation by the plant are in balance, or, as suggested by the strong reaction of 35S:CKX plants, that other CKs are at play. At early time points of the interaction, activation of gibberellin 2-oxidase presumably installs a local hormonal setting favorable for meristematic activity that provokes leaf serrations. The results are discussed in the context of symptom development, evasion of plant defense, and the establishment of a specific niche by R. fascians.},
  author       = {Depuydt, Stephen and Dole\v{z}al, Karel and Van Lijsebettens, Maria and Moritz, Thomas and Holsters, Marcella and Vereecke, Danny},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {CYTOKININ BIOSYNTHESIS,ASYMMETRIC LEAVES1,KNOTTED1-LIKE HOMEOBOX GENE,MERISTEM ACTIVITY,LEAF DEVELOPMENT,CORYNEBACTERIUM-FASCIANS,SHOOT MERISTEMLESS,GIBBERELLIN METABOLISM,PLANT DEVELOPMENT,CELL-DIVISION},
  language     = {eng},
  number       = {3},
  pages        = {1267--1281},
  title        = {Modulation of the hormone setting by Rhodococcus fascians results in ectopic KNOX activation in Arabidopsis},
  url          = {http://dx.doi.org/10.1104/pp.107.113969},
  volume       = {146},
  year         = {2008},
}

Chicago
Depuydt, Stephen, Karel Doležal, Maria Van Lijsebettens, Thomas Moritz, Marcella Holsters, and Danny Vereecke. 2008. “Modulation of the Hormone Setting by Rhodococcus Fascians Results in Ectopic KNOX Activation in Arabidopsis.” Plant Physiology 146 (3): 1267–1281.
APA
Depuydt, Stephen, Doležal, K., Van Lijsebettens, M., Moritz, T., Holsters, M., & Vereecke, D. (2008). Modulation of the hormone setting by Rhodococcus fascians results in ectopic KNOX activation in Arabidopsis. PLANT PHYSIOLOGY, 146(3), 1267–1281.
Vancouver
1.
Depuydt S, Doležal K, Van Lijsebettens M, Moritz T, Holsters M, Vereecke D. Modulation of the hormone setting by Rhodococcus fascians results in ectopic KNOX activation in Arabidopsis. PLANT PHYSIOLOGY. 2008;146(3):1267–81.
MLA
Depuydt, Stephen, Karel Doležal, Maria Van Lijsebettens, et al. “Modulation of the Hormone Setting by Rhodococcus Fascians Results in Ectopic KNOX Activation in Arabidopsis.” PLANT PHYSIOLOGY 146.3 (2008): 1267–1281. Print.