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Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants

Eva Růčková, Jiri Friml UGent, Petra Procházková Schrumpfová and Jiří Fajkus (2008) PLANT MOLECULAR BIOLOGY. 66(6). p.637-646
abstract
Telomeres in many eukaryotes are maintained by telomerase in whose absence telomere shortening occurs. However, telomerase-deficient Arabidopsis thaliana mutants (Attert(-/-)) show extremely low rates of telomere shortening per plant generation (250-500 bp), which does not correspond to the expected outcome of replicative telomere shortening resulting from ca. 1,000 meristem cell divisions per seed-to-seed generation. To investigate the influence of the number of cell divisions per seed-to-seed generation, Attert(-/-) mutant plants were propagated from seeds coming either from the lower-most or the upper-most siliques (L- and U-plants) and the length of their telomeres were followed over several generations. The rate of telomere shortening was faster in U-plants, than in L-plants, as would be expected from their higher number of cell divisions per generation. However, this trend was observed only in telomeres whose initial length is relatively high and the differences decreased with progressive general telomere shortening over generations. But in generation 4, the L-plants frequently show a net telomere elongation, while the U-plants fail to do so. We propose that this is due to the activation of alternative telomere lengthening (ALT), a process which is activated in early embryonic development in both U- and L-plants, but is overridden in U-plants due to their higher number of cell divisions per generation. These data demonstrate what so far has only been speculated, that in the absence of telomerase, the number of cell divisions within one generation influences the control of telomere lengths. These results also reveal a fast and efficient activation of ALT mechanism(s) in response to the loss of telomerase activity and imply that ALT is probably involved also in normal plant development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ALT, alternative telomere lengthening, replicative telomere shortening, telomerase-deficient plants, SHOOT APICAL MERISTEM, ARABIDOPSIS-THALIANA, MAINTENANCE, GENOME, CELLS, DNA, HAPLOINSUFFICIENCY, DIFFERENTIATION, DYSFUNCTION, SEQUENCES
journal title
PLANT MOLECULAR BIOLOGY
Plant Mol.Biol.
volume
66
issue
6
pages
637 - 646
Web of Science type
Article
Web of Science id
000254114700006
JCR category
PLANT SCIENCES
JCR impact factor
3.541 (2008)
JCR rank
21/155 (2008)
JCR quartile
1 (2008)
ISSN
0167-4412
DOI
10.1007/s11103-008-9295-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
436404
handle
http://hdl.handle.net/1854/LU-436404
date created
2008-11-04 19:54:00
date last changed
2012-12-05 15:09:35
@article{436404,
  abstract     = {Telomeres in many eukaryotes are maintained by telomerase in whose absence telomere shortening occurs. However, telomerase-deficient Arabidopsis thaliana mutants (Attert(-/-)) show extremely low rates of telomere shortening per plant generation (250-500 bp), which does not correspond to the expected outcome of replicative telomere shortening resulting from ca. 1,000 meristem cell divisions per seed-to-seed generation. To investigate the influence of the number of cell divisions per seed-to-seed generation, Attert(-/-) mutant plants were propagated from seeds coming either from the lower-most or the upper-most siliques (L- and U-plants) and the length of their telomeres were followed over several generations. The rate of telomere shortening was faster in U-plants, than in L-plants, as would be expected from their higher number of cell divisions per generation. However, this trend was observed only in telomeres whose initial length is relatively high and the differences decreased with progressive general telomere shortening over generations. But in generation 4, the L-plants frequently show a net telomere elongation, while the U-plants fail to do so. We propose that this is due to the activation of alternative telomere lengthening (ALT), a process which is activated in early embryonic development in both U- and L-plants, but is overridden in U-plants due to their higher number of cell divisions per generation. These data demonstrate what so far has only been speculated, that in the absence of telomerase, the number of cell divisions within one generation influences the control of telomere lengths. These results also reveal a fast and efficient activation of ALT mechanism(s) in response to the loss of telomerase activity and imply that ALT is probably involved also in normal plant development.},
  author       = {R\r{u}\v{c}kov{\'a}, Eva and Friml, Jiri and Proch{\'a}zkov{\'a} Schrumpfov{\'a}, Petra and Fajkus, Ji\v{r}{\'i}},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keyword      = {ALT,alternative telomere lengthening,replicative telomere shortening,telomerase-deficient plants,SHOOT APICAL MERISTEM,ARABIDOPSIS-THALIANA,MAINTENANCE,GENOME,CELLS,DNA,HAPLOINSUFFICIENCY,DIFFERENTIATION,DYSFUNCTION,SEQUENCES},
  language     = {eng},
  number       = {6},
  pages        = {637--646},
  title        = {Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants},
  url          = {http://dx.doi.org/10.1007/s11103-008-9295-7},
  volume       = {66},
  year         = {2008},
}

Chicago
Růčková, Eva, Jiri Friml, Petra Procházková Schrumpfová, and Jiří Fajkus. 2008. “Role of Alternative Telomere Lengthening Unmasked in Telomerase Knock-out Mutant Plants.” Plant Molecular Biology 66 (6): 637–646.
APA
Růčková, E., Friml, J., Procházková Schrumpfová, P., & Fajkus, J. (2008). Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants. PLANT MOLECULAR BIOLOGY, 66(6), 637–646.
Vancouver
1.
Růčková E, Friml J, Procházková Schrumpfová P, Fajkus J. Role of alternative telomere lengthening unmasked in telomerase knock-out mutant plants. PLANT MOLECULAR BIOLOGY. 2008;66(6):637–46.
MLA
Růčková, Eva, Jiri Friml, Petra Procházková Schrumpfová, et al. “Role of Alternative Telomere Lengthening Unmasked in Telomerase Knock-out Mutant Plants.” PLANT MOLECULAR BIOLOGY 66.6 (2008): 637–646. Print.