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Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

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Abstract
Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
Keywords
ROOT, CELLS, ORGANIZATION, NETWORK, PROTEIN, ENDOCYTOSIS, EARLY ENDOSOMES, EFFLUX CARRIERS, PLANT DEVELOPMENT, ARABIDOPSIS-THALIANA, auxin efflux inhibitors, vesicle traffic, PIN proteins, plant development

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Chicago
Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, Motoki Tominaga, David G Robinson, Jiří Hašek, Tomasz Paciorek, et al. 2008. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” Proceedings of the National Academy of Sciences of the United States of America 105 (11): 4489–4494.
APA
Dhonukshe, P., Grigoriev, I., Fischer, R., Tominaga, M., Robinson, D. G., Hašek, J., Paciorek, T., et al. (2008). Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(11), 4489–4494.
Vancouver
1.
Dhonukshe P, Grigoriev I, Fischer R, Tominaga M, Robinson DG, Hašek J, et al. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(11):4489–94.
MLA
Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, et al. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105.11 (2008): 4489–4494. Print.
@article{436385,
  abstract     = {Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.},
  author       = {Dhonukshe, Pankaj and Grigoriev, Ilya and Fischer, Rainer and Tominaga, Motoki and Robinson, David G and Ha\v{s}ek, Ji\v{r}{\'i} and Paciorek, Tomasz and Petr{\'a}\v{s}ek, Jan and Seifertov{\'a}, Daniela and Tejos Ulloa, Ricardo and Meisel, Lee A and Za\v{z}{\'i}malov{\'a}, Eva and Gadella, Theodorus WJ, Jr and Stierhof, York-Dieter and Ueda, Takashi and Oiwa, Kazuhiro and Akhmanova, Anna and Brock, Roland and Spang, Anne and Friml, Jiri},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {ROOT,CELLS,ORGANIZATION,NETWORK,PROTEIN,ENDOCYTOSIS,EARLY ENDOSOMES,EFFLUX CARRIERS,PLANT DEVELOPMENT,ARABIDOPSIS-THALIANA,auxin efflux inhibitors,vesicle traffic,PIN proteins,plant development},
  language     = {eng},
  number       = {11},
  pages        = {4489--4494},
  title        = {Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes},
  url          = {http://dx.doi.org/10.1073/pnas.0711414105},
  volume       = {105},
  year         = {2008},
}

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