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Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

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Abstract
Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
Keywords
ROOT, CELLS, ORGANIZATION, NETWORK, PROTEIN, ENDOCYTOSIS, EARLY ENDOSOMES, EFFLUX CARRIERS, PLANT DEVELOPMENT, ARABIDOPSIS-THALIANA, auxin efflux inhibitors, vesicle traffic, PIN proteins, plant development

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MLA
Dhonukshe, Pankaj, et al. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 105, no. 11, 2008, pp. 4489–94, doi:10.1073/pnas.0711414105.
APA
Dhonukshe, P., Grigoriev, I., Fischer, R., Tominaga, M., Robinson, D. G., Hašek, J., … Friml, J. (2008). Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(11), 4489–4494. https://doi.org/10.1073/pnas.0711414105
Chicago author-date
Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, Motoki Tominaga, David G Robinson, Jiří Hašek, Tomasz Paciorek, et al. 2008. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 (11): 4489–94. https://doi.org/10.1073/pnas.0711414105.
Chicago author-date (all authors)
Dhonukshe, Pankaj, Ilya Grigoriev, Rainer Fischer, Motoki Tominaga, David G Robinson, Jiří Hašek, Tomasz Paciorek, Jan Petrášek, Daniela Seifertová, Ricardo Tejos Ulloa, Lee A Meisel, Eva Zažímalová, Theodorus WJ Gadella Jr, York-Dieter Stierhof, Takashi Ueda, Kazuhiro Oiwa, Anna Akhmanova, Roland Brock, Anne Spang, and Jiri Friml. 2008. “Auxin Transport Inhibitors Impair Vesicle Motility and Actin Cytoskeleton Dynamics in Diverse Eukaryotes.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105 (11): 4489–4494. doi:10.1073/pnas.0711414105.
Vancouver
1.
Dhonukshe P, Grigoriev I, Fischer R, Tominaga M, Robinson DG, Hašek J, et al. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(11):4489–94.
IEEE
[1]
P. Dhonukshe et al., “Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 105, no. 11, pp. 4489–4494, 2008.
@article{436385,
  abstract     = {{Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.}},
  author       = {{Dhonukshe, Pankaj and Grigoriev, Ilya and Fischer, Rainer and Tominaga, Motoki and Robinson, David G and Hašek, Jiří and Paciorek, Tomasz and Petrášek, Jan and Seifertová, Daniela and Tejos Ulloa, Ricardo and Meisel, Lee A and Zažímalová, Eva and Gadella, Theodorus WJ, Jr and Stierhof, York-Dieter and Ueda, Takashi and Oiwa, Kazuhiro and Akhmanova, Anna and Brock, Roland and Spang, Anne and Friml, Jiri}},
  issn         = {{0027-8424}},
  journal      = {{PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}},
  keywords     = {{ROOT,CELLS,ORGANIZATION,NETWORK,PROTEIN,ENDOCYTOSIS,EARLY ENDOSOMES,EFFLUX CARRIERS,PLANT DEVELOPMENT,ARABIDOPSIS-THALIANA,auxin efflux inhibitors,vesicle traffic,PIN proteins,plant development}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{4489--4494}},
  title        = {{Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes}},
  url          = {{http://doi.org/10.1073/pnas.0711414105}},
  volume       = {{105}},
  year         = {{2008}},
}

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