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ARF GEF-dependent transcytosis and polar delivery of PIN auxin carriers in Arabidopsis

Jürgen Kleine-Vehn UGent, Pankaj Dhonukshe UGent, Michael Sauer, Philip B Brewer, Justyna Wiśniewska, Tomasz Paciorek, Eva Benkova UGent and Jiri Friml UGent (2008) CURRENT BIOLOGY. 18(7). p.526-531
abstract
Cell polarity manifested by the polar cargo delivery to different plasma-membrane domains is a fundamental feature of multicellular organisms. Pathways for polar delivery have been identified in animals; prominent among them is transcytosis, which involves cargo movement between different sides of the cell [1]. PIN transporters are prominent polar cargoes in plants, whose polar subcellular localization determines the directional flow of the signaling molecule auxin [2, 3]. In this study, we address the cellular mechanisms of PIN polar targeting and dynamic polarity changes. We show that apical and basal PIN targeting pathways are interconnected but molecularly distinct by means of ARF GEF vesicle-trafficking regulators. Pharmacological or genetic interference with the Arabidopsis ARF GEF GNOM leads specifically to apicalization of basal cargoes such as PIN1. We visualize the translocation of PIN proteins between the opposite sides of polarized cells in vivo and show that this PIN transcytosis occurs by endocytic recycling and alternative recruitment of the same cargo molecules by apical and basal targeting machineries. Our data suggest that an ARF GEF-dependent transcytosis-like mechanism is operational in plants and provides a plausible mechanism to trigger changes in PIN polarity and hence auxin fluxes during embryogenesis and organogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRAFFICKING, CANALIZATION, GNOM, CELLS, FLOW, GRADIENTS, ENDOCYTOSIS, TRANSPORT, LOCALIZATION, EFFLUX
journal title
CURRENT BIOLOGY
Curr. Biol.
volume
18
issue
7
pages
526 - 531
Web of Science type
Article
Web of Science id
000254846000030
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
10.777 (2008)
JCR rank
19/276 (2008)
JCR quartile
1 (2008)
ISSN
0960-9822
DOI
10.1016/j.cub.2008.03.021
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
436379
handle
http://hdl.handle.net/1854/LU-436379
date created
2008-11-04 19:51:00
date last changed
2013-09-17 11:23:22
@article{436379,
  abstract     = {Cell polarity manifested by the polar cargo delivery to different plasma-membrane domains is a fundamental feature of multicellular organisms. Pathways for polar delivery have been identified in animals; prominent among them is transcytosis, which involves cargo movement between different sides of the cell [1]. PIN transporters are prominent polar cargoes in plants, whose polar subcellular localization determines the directional flow of the signaling molecule auxin [2, 3]. In this study, we address the cellular mechanisms of PIN polar targeting and dynamic polarity changes. We show that apical and basal PIN targeting pathways are interconnected but molecularly distinct by means of ARF GEF vesicle-trafficking regulators. Pharmacological or genetic interference with the Arabidopsis ARF GEF GNOM leads specifically to apicalization of basal cargoes such as PIN1. We visualize the translocation of PIN proteins between the opposite sides of polarized cells in vivo and show that this PIN transcytosis occurs by endocytic recycling and alternative recruitment of the same cargo molecules by apical and basal targeting machineries. Our data suggest that an ARF GEF-dependent transcytosis-like mechanism is operational in plants and provides a plausible mechanism to trigger changes in PIN polarity and hence auxin fluxes during embryogenesis and organogenesis.},
  author       = {Kleine-Vehn, J{\"u}rgen and Dhonukshe, Pankaj and Sauer, Michael and Brewer, Philip B and Wi\'{s}niewska, Justyna and Paciorek, Tomasz and Benkova, Eva and Friml, Jiri},
  issn         = {0960-9822},
  journal      = {CURRENT BIOLOGY},
  keyword      = {TRAFFICKING,CANALIZATION,GNOM,CELLS,FLOW,GRADIENTS,ENDOCYTOSIS,TRANSPORT,LOCALIZATION,EFFLUX},
  language     = {eng},
  number       = {7},
  pages        = {526--531},
  title        = {ARF GEF-dependent transcytosis and polar delivery of PIN auxin carriers in Arabidopsis},
  url          = {http://dx.doi.org/10.1016/j.cub.2008.03.021},
  volume       = {18},
  year         = {2008},
}

Chicago
Kleine-Vehn, Jürgen, Pankaj Dhonukshe, Michael Sauer, Philip B Brewer, Justyna Wiśniewska, Tomasz Paciorek, Eva Benkova, and Jiri Friml. 2008. “ARF GEF-dependent Transcytosis and Polar Delivery of PIN Auxin Carriers in Arabidopsis.” Current Biology 18 (7): 526–531.
APA
Kleine-Vehn, J., Dhonukshe, P., Sauer, M., Brewer, P. B., Wiśniewska, J., Paciorek, T., Benkova, E., et al. (2008). ARF GEF-dependent transcytosis and polar delivery of PIN auxin carriers in Arabidopsis. CURRENT BIOLOGY, 18(7), 526–531.
Vancouver
1.
Kleine-Vehn J, Dhonukshe P, Sauer M, Brewer PB, Wiśniewska J, Paciorek T, et al. ARF GEF-dependent transcytosis and polar delivery of PIN auxin carriers in Arabidopsis. CURRENT BIOLOGY. 2008;18(7):526–31.
MLA
Kleine-Vehn, Jürgen, Pankaj Dhonukshe, Michael Sauer, et al. “ARF GEF-dependent Transcytosis and Polar Delivery of PIN Auxin Carriers in Arabidopsis.” CURRENT BIOLOGY 18.7 (2008): 526–531. Print.