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Auxin acts as a local morphogenetic trigger to specify lateral root founder cells

Joseph G Dubrovsky, Michael Sauer, Selene Napsucialy-Mendivil, Maria G Ivanchenko, Jiri Friml UGent, Svetlana Shishkova, John Celenza and Eva Benkova UGent (2008) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 105(25). p.8790-8794
abstract
Plants exhibit an exceptional adaptability to different environmental conditions. To a large extent, this adaptability depends on their ability to initiate and form new organs throughout their entire postembryonic life. Plant shoot and root systems unceasingly branch and form axillary shoots or lateral roots, respectively. The first event in the formation of a new organ is specification of founder cells. Several plant hormones, prominent among them auxin, have been implicated in the acquisition of founder cell identity by differentiated cells, but the mechanisms underlying this process are largely elusive. Here, we show that auxin and its local accumulation in root pericycle cells is a necessary and sufficient signal to respecify these cells into lateral root founder cells. Analysis of the alf4-1 mutant suggests that specification of founder cells and the subsequent activation of cell division leading to primordium formation represent two genetically separable events. Time-lapse experiments show that the activation of an auxin response is the earliest detectable event in founder cell specification. Accordingly, local activation of auxin response correlates absolutely with the acquisition of founder cell identity and precedes the actual formation of a lateral root primordium through patterned cell division. Local production and subsequent accumulation of auxin in single pericycle cells induced by Cre-Lox-based activation of auxin synthesis converts them into founder cells. Thus, auxin is the local instructive signal that is sufficient for acquisition of founder cell identity and can be considered a morphogenetic trigger in postembryonic plant organogenesis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
branching, cell identity, development, pericycle, plant hormones, ARABIDOPSIS-THALIANA, MERISTEM, PLANT, INITIATION, EXPRESSION, TRANSPORT, GRADIENTS, PATTERNS, ENCODES, ORGANS
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
105
issue
25
pages
8790 - 8794
Web of Science type
Article
Web of Science id
000257185700055
ISSN
0027-8424
DOI
10.1073/pnas.0712307105
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
436370
handle
http://hdl.handle.net/1854/LU-436370
date created
2008-11-04 19:50:00
date last changed
2012-12-05 14:38:27
@article{436370,
  abstract     = {Plants exhibit an exceptional adaptability to different environmental conditions. To a large extent, this adaptability depends on their ability to initiate and form new organs throughout their entire postembryonic life. Plant shoot and root systems unceasingly branch and form axillary shoots or lateral roots, respectively. The first event in the formation of a new organ is specification of founder cells. Several plant hormones, prominent among them auxin, have been implicated in the acquisition of founder cell identity by differentiated cells, but the mechanisms underlying this process are largely elusive. Here, we show that auxin and its local accumulation in root pericycle cells is a necessary and sufficient signal to respecify these cells into lateral root founder cells. Analysis of the alf4-1 mutant suggests that specification of founder cells and the subsequent activation of cell division leading to primordium formation represent two genetically separable events. Time-lapse experiments show that the activation of an auxin response is the earliest detectable event in founder cell specification. Accordingly, local activation of auxin response correlates absolutely with the acquisition of founder cell identity and precedes the actual formation of a lateral root primordium through patterned cell division. Local production and subsequent accumulation of auxin in single pericycle cells induced by Cre-Lox-based activation of auxin synthesis converts them into founder cells. Thus, auxin is the local instructive signal that is sufficient for acquisition of founder cell identity and can be considered a morphogenetic trigger in postembryonic plant organogenesis.},
  author       = {Dubrovsky, Joseph G and Sauer, Michael and Napsucialy-Mendivil, Selene and Ivanchenko, Maria G and Friml, Jiri and Shishkova, Svetlana and Celenza, John and Benkova, Eva},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {branching,cell identity,development,pericycle,plant hormones,ARABIDOPSIS-THALIANA,MERISTEM,PLANT,INITIATION,EXPRESSION,TRANSPORT,GRADIENTS,PATTERNS,ENCODES,ORGANS},
  language     = {eng},
  number       = {25},
  pages        = {8790--8794},
  title        = {Auxin acts as a local morphogenetic trigger to specify lateral root founder cells},
  url          = {http://dx.doi.org/10.1073/pnas.0712307105},
  volume       = {105},
  year         = {2008},
}

Chicago
Dubrovsky, Joseph G, Michael Sauer, Selene Napsucialy-Mendivil, Maria G Ivanchenko, Jiri Friml, Svetlana Shishkova, John Celenza, and Eva Benkova. 2008. “Auxin Acts as a Local Morphogenetic Trigger to Specify Lateral Root Founder Cells.” Proceedings of the National Academy of Sciences of the United States of America 105 (25): 8790–8794.
APA
Dubrovsky, J. G., Sauer, M., Napsucialy-Mendivil, S., Ivanchenko, M. G., Friml, J., Shishkova, S., Celenza, J., et al. (2008). Auxin acts as a local morphogenetic trigger to specify lateral root founder cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(25), 8790–8794.
Vancouver
1.
Dubrovsky JG, Sauer M, Napsucialy-Mendivil S, Ivanchenko MG, Friml J, Shishkova S, et al. Auxin acts as a local morphogenetic trigger to specify lateral root founder cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(25):8790–4.
MLA
Dubrovsky, Joseph G, Michael Sauer, Selene Napsucialy-Mendivil, et al. “Auxin Acts as a Local Morphogenetic Trigger to Specify Lateral Root Founder Cells.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105.25 (2008): 8790–8794. Print.