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Hemato-critical issues in quantitative analysis of dried blood spots: challenges and solutions

Pieter De Kesel (UGent) , Nele Sadones (UGent) , Sara Capiau (UGent) , Willy Lambert (UGent) and Christophe Stove (UGent)
(2013) BIOANALYSIS. 5(16). p.2023-2041
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Abstract
Dried blood spot (DBS) sampling for quantitative determination of drugs in blood has entered the bioanalytical arena at a fast pace during the last decade, primarily owing to progress in analytical instrumentation. Despite the many advantages associated with this new sampling strategy, several issues remain, of which the hematocrit issue is undoubtedly the most widely discussed challenge, since strongly deviating hematocrit values may significantly impact DBS-based quantitation. In this review, an overview is given of the different aspects of the hematocrit problem' in quantitative DBS analysis. The different strategies that try to cope with this problem are discussed, along with their potential and limitations. Implementation of some of these strategies in practice may help to overcome this important hurdle in DBS assays, further allowing DBS to become an established part of routine quantitative bioanalysis.
Keywords
PLASMA SPOTS, LC-MS/MS, FILTER-PAPER, TANDEM MASS-SPECTROMETRY, GAMMA-HYDROXYBUTYRIC ACID, CLINICAL VALIDATION, BIOANALYTICAL METHOD, HEMOGLOBIN VALUES, WHOLE-BLOOD, PHARMACOKINETIC CONSIDERATIONS

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Citation

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MLA
De Kesel, Pieter, Nele Sadones, Sara Capiau, et al. “Hemato-critical Issues in Quantitative Analysis of Dried Blood Spots: Challenges and Solutions.” BIOANALYSIS 5.16 (2013): 2023–2041. Print.
APA
De Kesel, P., Sadones, N., Capiau, S., Lambert, W., & Stove, C. (2013). Hemato-critical issues in quantitative analysis of dried blood spots: challenges and solutions. BIOANALYSIS, 5(16), 2023–2041.
Chicago author-date
De Kesel, Pieter, Nele Sadones, Sara Capiau, Willy Lambert, and Christophe Stove. 2013. “Hemato-critical Issues in Quantitative Analysis of Dried Blood Spots: Challenges and Solutions.” Bioanalysis 5 (16): 2023–2041.
Chicago author-date (all authors)
De Kesel, Pieter, Nele Sadones, Sara Capiau, Willy Lambert, and Christophe Stove. 2013. “Hemato-critical Issues in Quantitative Analysis of Dried Blood Spots: Challenges and Solutions.” Bioanalysis 5 (16): 2023–2041.
Vancouver
1.
De Kesel P, Sadones N, Capiau S, Lambert W, Stove C. Hemato-critical issues in quantitative analysis of dried blood spots: challenges and solutions. BIOANALYSIS. 2013;5(16):2023–41.
IEEE
[1]
P. De Kesel, N. Sadones, S. Capiau, W. Lambert, and C. Stove, “Hemato-critical issues in quantitative analysis of dried blood spots: challenges and solutions,” BIOANALYSIS, vol. 5, no. 16, pp. 2023–2041, 2013.
@article{4363326,
  abstract     = {{Dried blood spot (DBS) sampling for quantitative determination of drugs in blood has entered the bioanalytical arena at a fast pace during the last decade, primarily owing to progress in analytical instrumentation. Despite the many advantages associated with this new sampling strategy, several issues remain, of which the hematocrit issue is undoubtedly the most widely discussed challenge, since strongly deviating hematocrit values may significantly impact DBS-based quantitation. In this review, an overview is given of the different aspects of the hematocrit problem' in quantitative DBS analysis. The different strategies that try to cope with this problem are discussed, along with their potential and limitations. Implementation of some of these strategies in practice may help to overcome this important hurdle in DBS assays, further allowing DBS to become an established part of routine quantitative bioanalysis.}},
  author       = {{De Kesel, Pieter and Sadones, Nele and Capiau, Sara and Lambert, Willy and Stove, Christophe}},
  issn         = {{1757-6180}},
  journal      = {{BIOANALYSIS}},
  keywords     = {{PLASMA SPOTS,LC-MS/MS,FILTER-PAPER,TANDEM MASS-SPECTROMETRY,GAMMA-HYDROXYBUTYRIC ACID,CLINICAL VALIDATION,BIOANALYTICAL METHOD,HEMOGLOBIN VALUES,WHOLE-BLOOD,PHARMACOKINETIC CONSIDERATIONS}},
  language     = {{eng}},
  number       = {{16}},
  pages        = {{2023--2041}},
  title        = {{Hemato-critical issues in quantitative analysis of dried blood spots: challenges and solutions}},
  url          = {{http://dx.doi.org/10.4155/BIO.13.156}},
  volume       = {{5}},
  year         = {{2013}},
}

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