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SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis thaliana

(2014) PLANT JOURNAL. 77(5). p.782-794
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
In meiosis, chromosome cohesion is maintained by the cohesin complex, which is released in a two-step manner. At meiosis I, the meiosis-specific cohesin subunit Rec8 is cleaved by the protease Separase along chromosome arms, allowing homologous chromosome segregation. Next, in meiosis II, cleavage of the remaining centromere cohesin results in separation of the sister chromatids. In eukaryotes, protection of centromeric cohesion in meiosis I is mediated by SHUGOSHINs (SGOs). The Arabidopsis genome contains two SGO homologs. Here we demonstrate that Atsgo1 mutants show a premature loss of cohesion of sister chromatid centromeres at anaphase I and that AtSGO2 partially rescues this loss of cohesion. In addition to SGOs, we characterize PATRONUS which is specifically required for the maintenance of cohesion of sister chromatid centromeres in meiosis II. In contrast to the Atsgo1 Atsgo2 double mutant, patronus T-DNA insertion mutants only display loss of sister chromatid cohesion after meiosis I, and additionally show disorganized spindles, resulting in defects in chromosome segregation in meiosis. This leads to reduced fertility and aneuploid offspring. Furthermore, we detect aneuploidy in sporophytic tissue, indicating a role for PATRONUS in chromosome segregation in somatic cells. Thus, ploidy stability is preserved in Arabidopsis by PATRONUS during both meiosis and mitosis.
Keywords
ALIGNMENT, CHROMOSOME SEGREGATION, MEIOSIS-II, COMPLEX, MITOSIS, MECHANISMS, MAINTENANCE, CELL-CYCLE, SISTER-CHROMATID COHESION, PHOSPHATASE 2A, Arabidopsis thaliana, chromosome segregation, centromere, meiosis, sister chromatids, cohesion

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Citation

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Chicago
Zamariola, Linda, Nico De Storme, Katrijn Vannerum, Klaas Vandepoele, Susan J Armstrong, F Christopher H Franklin, and Danny Geelen. 2014. “SHUGOSHINs and PATRONUS Protect Meiotic Centromere Cohesion in Arabidopsis Thaliana.” Plant Journal 77 (5): 782–794.
APA
Zamariola, L., De Storme, N., Vannerum, K., Vandepoele, K., Armstrong, S. J., Franklin, F. C. H., & Geelen, D. (2014). SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis thaliana. PLANT JOURNAL, 77(5), 782–794.
Vancouver
1.
Zamariola L, De Storme N, Vannerum K, Vandepoele K, Armstrong SJ, Franklin FCH, et al. SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis thaliana. PLANT JOURNAL. 2014;77(5):782–94.
MLA
Zamariola, Linda, Nico De Storme, Katrijn Vannerum, et al. “SHUGOSHINs and PATRONUS Protect Meiotic Centromere Cohesion in Arabidopsis Thaliana.” PLANT JOURNAL 77.5 (2014): 782–794. Print.
@article{4359021,
  abstract     = {In meiosis, chromosome cohesion is maintained by the cohesin complex, which is released in a two-step manner. At meiosis I, the meiosis-specific cohesin subunit Rec8 is cleaved by the protease Separase along chromosome arms, allowing homologous chromosome segregation. Next, in meiosis II, cleavage of the remaining centromere cohesin results in separation of the sister chromatids. In eukaryotes, protection of centromeric cohesion in meiosis I is mediated by SHUGOSHINs (SGOs). The Arabidopsis genome contains two SGO homologs. Here we demonstrate that Atsgo1 mutants show a premature loss of cohesion of sister chromatid centromeres at anaphase I and that AtSGO2 partially rescues this loss of cohesion. In addition to SGOs, we characterize PATRONUS which is specifically required for the maintenance of cohesion of sister chromatid centromeres in meiosis II. In contrast to the Atsgo1 Atsgo2 double mutant, patronus T-DNA insertion mutants only display loss of sister chromatid cohesion after meiosis I, and additionally show disorganized spindles, resulting in defects in chromosome segregation in meiosis. This leads to reduced fertility and aneuploid offspring. Furthermore, we detect aneuploidy in sporophytic tissue, indicating a role for PATRONUS in chromosome segregation in somatic cells. Thus, ploidy stability is preserved in Arabidopsis by PATRONUS during both meiosis and mitosis.},
  author       = {Zamariola, Linda and De Storme, Nico and Vannerum, Katrijn and Vandepoele, Klaas and Armstrong, Susan J and Franklin, F Christopher H and Geelen, Danny},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {ALIGNMENT,CHROMOSOME SEGREGATION,MEIOSIS-II,COMPLEX,MITOSIS,MECHANISMS,MAINTENANCE,CELL-CYCLE,SISTER-CHROMATID COHESION,PHOSPHATASE 2A,Arabidopsis thaliana,chromosome segregation,centromere,meiosis,sister chromatids,cohesion},
  language     = {eng},
  number       = {5},
  pages        = {782--794},
  title        = {SHUGOSHINs and PATRONUS protect meiotic centromere cohesion in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1111/tpj.12432},
  volume       = {77},
  year         = {2014},
}

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