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Perfrin, a novel bacteriocin associated with netB positive Clostridium perfringens strains from broilers with necrotic eneteritis

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Abstract
Necrotic enteritis in broiler chickens is associated with netB positive Clostridium perfringens type A strains. It is known that C. perfringens strains isolated from outbreaks of necrotic enteritis are more capable of secreting factors inhibiting growth of other C. perfringens strains than strains isolated from the gut of healthy chickens. This characteristic could lead to extensive and selective presence of a strain that contains the genetic make-up enabling to secrete toxins that cause gut lesions. This report describes the discovery, purification, characterization and recombinant expression of a novel bacteriocin, referred to as perfrin, produced by a necrotic enteritis-associated netB-positive C. perfringens strain. Perfrin is a 11.5 kDa C-terminal fragment of a 22.9 kDa protein and showed no sequence homology to any currently known bacteriocin. The 11.5 kDa fragment can be cloned into Escherichia coli, and expression yielded an active peptide. PCR detection of the gene showed its presence in 10 netB-positive C. perfringens strains of broiler origin, and not in other C. perfringens strains tested (isolated from broilers, cattle, sheep, pigs, and humans). Perfrin and NetB are not located on the same genetic element since NetB is plasmid-encoded and perfrin is not. The bacteriocin has bactericidal activity over a wide pH-range but is thermolabile and sensitive to proteolytic digestion (trypsin, proteinase K). C. perfringens bacteriocins, such as perfrin, can be considered as an additional factor involved in the pathogenesis of necrotic enteritis in broilers.
Keywords
GENOME SEQUENCE, MEMBRANE INTERACTION, CHICKENS, COCULTURE

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Chicago
Timbermont, Leen, Lina De Smet, Filip Van Nieuwerburgh, Valeria R Pareira, Gonzalez Van Driessche, Freddy Haesebrouck, Richard Ducatelle, et al. 2014. “Perfrin, a Novel Bacteriocin Associated with netB Positive Clostridium Perfringens Strains from Broilers with Necrotic Eneteritis.” Veterinary Research 45.
APA
Timbermont, L., De Smet, L., Van Nieuwerburgh, F., Pareira, V. R., Van Driessche, G., Haesebrouck, F., Ducatelle, R., et al. (2014). Perfrin, a novel bacteriocin associated with netB positive Clostridium perfringens strains from broilers with necrotic eneteritis. VETERINARY RESEARCH, 45.
Vancouver
1.
Timbermont L, De Smet L, Van Nieuwerburgh F, Pareira VR, Van Driessche G, Haesebrouck F, et al. Perfrin, a novel bacteriocin associated with netB positive Clostridium perfringens strains from broilers with necrotic eneteritis. VETERINARY RESEARCH. 2014;45.
MLA
Timbermont, Leen, Lina De Smet, Filip Van Nieuwerburgh, et al. “Perfrin, a Novel Bacteriocin Associated with netB Positive Clostridium Perfringens Strains from Broilers with Necrotic Eneteritis.” VETERINARY RESEARCH 45 (2014): n. pag. Print.
@article{4358779,
  abstract     = {Necrotic enteritis in broiler chickens is associated with netB positive Clostridium perfringens type A strains. It is known that C. perfringens strains isolated from outbreaks of necrotic enteritis are more capable of secreting factors inhibiting growth of other C. perfringens strains than strains isolated from the gut of healthy chickens. This characteristic could lead to extensive and selective presence of a strain that contains the genetic make-up enabling to secrete toxins that cause gut lesions. This report describes the discovery, purification, characterization and recombinant expression of a novel bacteriocin, referred to as perfrin, produced by a necrotic enteritis-associated netB-positive C. perfringens strain. Perfrin is a 11.5 kDa C-terminal fragment of a 22.9 kDa protein and showed no sequence homology to any currently known bacteriocin. The 11.5 kDa fragment can be cloned into Escherichia coli, and expression yielded an active peptide. PCR detection of the gene showed its presence in 10 netB-positive C. perfringens strains of broiler origin, and not in other C. perfringens strains tested (isolated from broilers, cattle, sheep, pigs, and humans). Perfrin and NetB are not located on the same genetic element since NetB is plasmid-encoded and perfrin is not. The bacteriocin has bactericidal activity over a wide pH-range but is thermolabile and sensitive to proteolytic digestion (trypsin, proteinase K). C. perfringens bacteriocins, such as perfrin, can be considered as an additional factor involved in the pathogenesis of necrotic enteritis in broilers.},
  articleno    = {40},
  author       = {Timbermont, Leen and De Smet, Lina and Van Nieuwerburgh, Filip and Pareira, Valeria R and Van Driessche, Gonzalez and Haesebrouck, Freddy and Ducatelle, Richard and Prescott, John and Deforce, Dieter and Devreese, Bart and Van Immerseel, Filip},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  language     = {eng},
  pages        = {10},
  title        = {Perfrin, a novel bacteriocin associated with netB positive Clostridium perfringens strains from broilers with necrotic eneteritis},
  url          = {http://dx.doi.org/10.1186/1297-9716-45-40},
  volume       = {45},
  year         = {2014},
}

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