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Porcine, murine and human sialoadhesin (Sn/Siglec-1/CD169): portals for porcine reproductive and respiratory syndrome virus entry into target cells

Wander Van Breedam (UGent) , Mieke Verbeeck (UGent) , Isaura Christiaens (UGent) , Hanne Van Gorp (UGent) and Hans Nauwynck (UGent)
(2013) JOURNAL OF GENERAL VIROLOGY. 94(9). p.1955-1960
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Abstract
Porcine sialoadhesin (pSn; a sialic acid-binding lectin) and porcine CD163 (pCD163) are molecules that facilitate infectious entry of porcine reproductive and respiratory syndrome virus (PRRSV) into alveolar macrophages. In this study, it was shown that murine Sn (mSn) and human Sn (hSn), like pSn, can promote PRRSV infection of pCD163-expressing cells. Intact sialic acid-binding domains are crucial, since non-sialic acid-binding mutants of pSn, mSn and hSn did not promote infection. Endodomain-deletion mutants of pSn, mSn and hSn promoted PRRSV infection less efficiently, but also showed markedly reduced expression levels, making further research into the potential role of the Sn endodomain in PRRSV receptor activity necessary. These data further complement our knowledge on Sn as an important PRRSV receptor, and suggest - in combination with other published data - that species differences in the main PRRSV entry mediators Sn and CD163 do not account for the strict host species specificity displayed by the virus.
Keywords
ACID-BINDING-SITE, SITE-DIRECTED MUTAGENESIS, SIALIC-ACID, ALVEOLAR MACROPHAGES, IMMUNE-SYSTEM, RECEPTOR, CD163, IDENTIFICATION, SUSCEPTIBILITY, DOMAIN

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Chicago
Van Breedam, Wander, Mieke Verbeeck, Isaura Christiaens, Hanne Van Gorp, and Hans Nauwynck. 2013. “Porcine, Murine and Human Sialoadhesin (Sn/Siglec-1/CD169): Portals for Porcine Reproductive and Respiratory Syndrome Virus Entry into Target Cells.” Journal of General Virology 94 (9): 1955–1960.
APA
Van Breedam, W., Verbeeck, M., Christiaens, I., Van Gorp, H., & Nauwynck, H. (2013). Porcine, murine and human sialoadhesin (Sn/Siglec-1/CD169): portals for porcine reproductive and respiratory syndrome virus entry into target cells. JOURNAL OF GENERAL VIROLOGY, 94(9), 1955–1960.
Vancouver
1.
Van Breedam W, Verbeeck M, Christiaens I, Van Gorp H, Nauwynck H. Porcine, murine and human sialoadhesin (Sn/Siglec-1/CD169): portals for porcine reproductive and respiratory syndrome virus entry into target cells. JOURNAL OF GENERAL VIROLOGY. 2013;94(9):1955–60.
MLA
Van Breedam, Wander, Mieke Verbeeck, Isaura Christiaens, et al. “Porcine, Murine and Human Sialoadhesin (Sn/Siglec-1/CD169): Portals for Porcine Reproductive and Respiratory Syndrome Virus Entry into Target Cells.” JOURNAL OF GENERAL VIROLOGY 94.9 (2013): 1955–1960. Print.
@article{4357020,
  abstract     = {Porcine sialoadhesin (pSn; a sialic acid-binding lectin) and porcine CD163 (pCD163) are molecules that facilitate infectious entry of porcine reproductive and respiratory syndrome virus (PRRSV) into alveolar macrophages. In this study, it was shown that murine Sn (mSn) and human Sn (hSn), like pSn, can promote PRRSV infection of pCD163-expressing cells. Intact sialic acid-binding domains are crucial, since non-sialic acid-binding mutants of pSn, mSn and hSn did not promote infection. Endodomain-deletion mutants of pSn, mSn and hSn promoted PRRSV infection less efficiently, but also showed markedly reduced expression levels, making further research into the potential role of the Sn endodomain in PRRSV receptor activity necessary. These data further complement our knowledge on Sn as an important PRRSV receptor, and suggest - in combination with other published data - that species differences in the main PRRSV entry mediators Sn and CD163 do not account for the strict host species specificity displayed by the virus.},
  author       = {Van Breedam, Wander and Verbeeck, Mieke and Christiaens, Isaura and Van Gorp, Hanne and Nauwynck, Hans},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  language     = {eng},
  number       = {9},
  pages        = {1955--1960},
  title        = {Porcine, murine and human sialoadhesin (Sn/Siglec-1/CD169): portals for porcine reproductive and respiratory syndrome virus entry into target cells},
  url          = {http://dx.doi.org/10.1099/vir.0.053082-0},
  volume       = {94},
  year         = {2013},
}

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