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Prevalence, risk factors and genetic diversity of methicillin-resistant Staphylococcus aureus carried by humans and animals across livestock production sectors

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Abstract
This study aimed to assess the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in animals and humans on veal, dairy, beef and broiler farms and to compare the risk for human MRSA carriage with that of strictly horticulture farmers. The genetic background, resistance phenotypes and genotypes and toxin gene content of the isolated MRSA strains were compared with MRSA collected on MRSA clonal complex (CC)398-positive pig farms. MRSA carriage isolates were genotyped (spa, SCCmec and multilocus sequence typing), resistance to 16 antimicrobials was determined and resistance and toxin genes were detected. MRSA carriage rates were higher (P0.01) on veal farms (calves, 64; farmers, 72) compared with on dairy (cows, 1), beef (cows, 5; farmers, 11), broiler (pooled broths, 5; farmers, 3) and horticulture (farmers, 3) farms. The intensity of animal contact was identified as a risk factor for human MRSA carriage. The vast majority of MRSA (n344), including those from pigs, were CC398 (98). SCCmec V(5C2), V(5C25)c, IV(2B) and IV(2B5) predominated. MRSA CC130 and CC599 carrying mecC were detected in beef and dairy cattle. MRSA from veal calves were significantly more resistant than MRSA from pigs (P0.01). A few isolates, including mecC-carrying MRSA, harboured pyrogenic superantigen toxins. Human- and animal-derived MRSA from individual farms showed similar characteristics. This systematic cross-sector survey revealed a high prevalence of multiresistant livestock-associated MRSA on Belgian veal calf farms as compared with other farm types. MRSA harbouring mecC was detected at a low frequency in beef and dairy cows, but not in humans.
Keywords
antimicrobial resistance epidemiology, food animals, ANTIMICROBIAL RESISTANCE, HOMOLOG MECA(LGA251), MECA HOMOLOG, ST398, BELGIUM, CALVES, STRAINS, SWINE, public health, molecular epidemiology, MRSA, S. aureus

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Citation

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Chicago
Vandendriessche, Stien, Wannes Vanderhaeghen, Filomena Valente Soares, Marie Hallin, Boudewijn Catry, Katleen Hermans, Patrick Butaye, Freddy Haesebrouck, Marc J Struelens, and Olivier Denis. 2013. “Prevalence, Risk Factors and Genetic Diversity of Methicillin-resistant Staphylococcus Aureus Carried by Humans and Animals Across Livestock Production Sectors.” Journal of Antimicrobial Chemotherapy 68 (7): 1510–1516.
APA
Vandendriessche, Stien, Vanderhaeghen, W., Soares, F. V., Hallin, M., Catry, B., Hermans, K., Butaye, P., et al. (2013). Prevalence, risk factors and genetic diversity of methicillin-resistant Staphylococcus aureus carried by humans and animals across livestock production sectors. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 68(7), 1510–1516.
Vancouver
1.
Vandendriessche S, Vanderhaeghen W, Soares FV, Hallin M, Catry B, Hermans K, et al. Prevalence, risk factors and genetic diversity of methicillin-resistant Staphylococcus aureus carried by humans and animals across livestock production sectors. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2013;68(7):1510–6.
MLA
Vandendriessche, Stien, Wannes Vanderhaeghen, Filomena Valente Soares, et al. “Prevalence, Risk Factors and Genetic Diversity of Methicillin-resistant Staphylococcus Aureus Carried by Humans and Animals Across Livestock Production Sectors.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 68.7 (2013): 1510–1516. Print.
@article{4356315,
  abstract     = {This study aimed to assess the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in animals and humans on veal, dairy, beef and broiler farms and to compare the risk for human MRSA carriage with that of strictly horticulture farmers. The genetic background, resistance phenotypes and genotypes and toxin gene content of the isolated MRSA strains were compared with MRSA collected on MRSA clonal complex (CC)398-positive pig farms.
MRSA carriage isolates were genotyped (spa, SCCmec and multilocus sequence typing), resistance to 16 antimicrobials was determined and resistance and toxin genes were detected.
MRSA carriage rates were higher (P0.01) on veal farms (calves, 64; farmers, 72) compared with on dairy (cows, 1), beef (cows, 5; farmers, 11), broiler (pooled broths, 5; farmers, 3) and horticulture (farmers, 3) farms. The intensity of animal contact was identified as a risk factor for human MRSA carriage. The vast majority of MRSA (n344), including those from pigs, were CC398 (98). SCCmec V(5C2), V(5C25)c, IV(2B) and IV(2B5) predominated. MRSA CC130 and CC599 carrying mecC were detected in beef and dairy cattle. MRSA from veal calves were significantly more resistant than MRSA from pigs (P0.01). A few isolates, including mecC-carrying MRSA, harboured pyrogenic superantigen toxins. Human- and animal-derived MRSA from individual farms showed similar characteristics.
This systematic cross-sector survey revealed a high prevalence of multiresistant livestock-associated MRSA on Belgian veal calf farms as compared with other farm types. MRSA harbouring mecC was detected at a low frequency in beef and dairy cows, but not in humans.},
  author       = {Vandendriessche, Stien and Vanderhaeghen, Wannes and Soares, Filomena Valente and Hallin, Marie and Catry, Boudewijn and Hermans, Katleen and Butaye, Patrick and Haesebrouck, Freddy and Struelens, Marc J and Denis, Olivier},
  issn         = {0305-7453},
  journal      = {JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY},
  keyword      = {antimicrobial resistance epidemiology,food animals,ANTIMICROBIAL RESISTANCE,HOMOLOG MECA(LGA251),MECA HOMOLOG,ST398,BELGIUM,CALVES,STRAINS,SWINE,public health,molecular epidemiology,MRSA,S. aureus},
  language     = {eng},
  number       = {7},
  pages        = {1510--1516},
  title        = {Prevalence, risk factors and genetic diversity of methicillin-resistant Staphylococcus aureus carried by humans and animals across livestock production sectors},
  url          = {http://dx.doi.org/10.1093/jac/dkt047},
  volume       = {68},
  year         = {2013},
}

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