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Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources

Catharina De Schauwer UGent, Karen Goossens, Sofie Piepers UGent, Maarten Hoogewijs, Jan Govaere UGent, Katrien Smits UGent, Evelyne Meyer UGent, Ann Van Soom UGent and Gerlinde Van de Walle (2014) STEM CELL RESEARCH & THERAPY. 5.
abstract
Introduction: Mesenchymal stromal cells (MSCs) have been extensively studied for their promising capabilities in regenerative medicine. Although bone marrow is the best-known source for isolating equine MSCs, non-invasive alternative sources such as umbilical cord blood (UCB), umbilical cord matrix (UCM), and peripheral blood (PB) have also been reported. Methods: Equine MSCs from three non-invasive alternative sources were isolated from six individual mares (PB) and their foals (UCB and UCM) at parturition. To minimize inter-horse variability, the samples from the three sources were matched within the same mare and for UCB and UCM even within the same foal from that specific mare. The following parameters were analyzed: (i) success rate of isolation, (ii) proliferation capacity, (iii) tri-lineage differentiation ability, (iv) immunophenotypical protein, and (v) immunomodulatory mRNA profiles. Linear regression models were fit to determine the association between the source of MSCs (UCB, UCM, PB) and (i) the moment of first observation, (ii) the moment of first passage, (iii) cell proliferation data, (iv) the expression of markers related to cell immunogenicity, and (v) the mRNA profile of immunomodulatory factors, except for hepatocyte growth factor (HGF) as no normal distribution could be obtained for the latter variable. To evaluate the association between the source of MSCs and the mRNA expression of HGF, the non-parametric Kruskal-Wallis test was performed instead. Results: While equine MSCs could be isolated from all the UCB and PB samples, isolation from UCM was successful in only two samples because of contamination issues. Proliferation data showed that equine MSCs from all three sources could be easily expanded, although UCB-derived MSCs appeared significantly faster in culture than PB- or UCM-derived MSCs. Equine MSCs from both UCB and PB could be differentiated toward the osteo-, chondro-, and adipogenic lineage, in contrast to UCM-derived MSCs in which only chondro-and adipogenic differentiation could be confirmed. Regardless of the source, equine MSCs expressed the immunomodulatory genes CD40, CD80, HGF, and transforming growth factor-beta (TGF beta). In contrast, no mRNA expression was found for CD86, indoleamine 2,3-dioxygenase (IDO), and tumor necrosis factor-alpha (TNF alpha). Conclusions: Whereas UCM seems less feasible because of the high contamination risks and low isolation success rates, UCB seems a promising alternative MSC source, especially when considering allogeneic MSC use.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
REAL-TIME PCR, UMBILICAL-CORD BLOOD, STEM-CELLS, BONE-MARROW, PERIPHERAL-BLOOD, ADIPOSE-TISSUE, ADULT, DIFFERENTIATION, REGENERATION, EXPRESSION
journal title
STEM CELL RESEARCH & THERAPY
Stem Cell Res. Ther.
volume
5
article number
6
pages
13 pages
Web of Science type
Article
Web of Science id
000331725600001
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
3.368 (2014)
JCR rank
36/123 (2014)
JCR quartile
2 (2014)
ISSN
1757-6512
DOI
10.1186/scrt395
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
4351758
handle
http://hdl.handle.net/1854/LU-4351758
date created
2014-04-02 15:58:12
date last changed
2016-12-21 15:42:08
@article{4351758,
  abstract     = {Introduction: Mesenchymal stromal cells (MSCs) have been extensively studied for their promising capabilities in regenerative medicine. Although bone marrow is the best-known source for isolating equine MSCs, non-invasive alternative sources such as umbilical cord blood (UCB), umbilical cord matrix (UCM), and peripheral blood (PB) have also been reported.
Methods: Equine MSCs from three non-invasive alternative sources were isolated from six individual mares (PB) and their foals (UCB and UCM) at parturition. To minimize inter-horse variability, the samples from the three sources were matched within the same mare and for UCB and UCM even within the same foal from that specific mare. The following parameters were analyzed: (i) success rate of isolation, (ii) proliferation capacity, (iii) tri-lineage differentiation ability, (iv) immunophenotypical protein, and (v) immunomodulatory mRNA profiles. Linear regression models were fit to determine the association between the source of MSCs (UCB, UCM, PB) and (i) the moment of first observation, (ii) the moment of first passage, (iii) cell proliferation data, (iv) the expression of markers related to cell immunogenicity, and (v) the mRNA profile of immunomodulatory factors, except for hepatocyte growth factor (HGF) as no normal distribution could be obtained for the latter variable. To evaluate the association between the source of MSCs and the mRNA expression of HGF, the non-parametric Kruskal-Wallis test was performed instead.
Results: While equine MSCs could be isolated from all the UCB and PB samples, isolation from UCM was successful in only two samples because of contamination issues. Proliferation data showed that equine MSCs from all three sources could be easily expanded, although UCB-derived MSCs appeared significantly faster in culture than PB- or UCM-derived MSCs. Equine MSCs from both UCB and PB could be differentiated toward the osteo-, chondro-, and adipogenic lineage, in contrast to UCM-derived MSCs in which only chondro-and adipogenic differentiation could be confirmed. Regardless of the source, equine MSCs expressed the immunomodulatory genes CD40, CD80, HGF, and transforming growth factor-beta (TGF beta). In contrast, no mRNA expression was found for CD86, indoleamine 2,3-dioxygenase (IDO), and tumor necrosis factor-alpha (TNF alpha).
Conclusions: Whereas UCM seems less feasible because of the high contamination risks and low isolation success rates, UCB seems a promising alternative MSC source, especially when considering allogeneic MSC use.},
  articleno    = {6},
  author       = {De Schauwer, Catharina and Goossens, Karen and Piepers, Sofie and Hoogewijs, Maarten and Govaere, Jan and Smits, Katrien and Meyer, Evelyne and Van Soom, Ann and Van de Walle, Gerlinde},
  issn         = {1757-6512},
  journal      = {STEM CELL RESEARCH \& THERAPY},
  keyword      = {REAL-TIME PCR,UMBILICAL-CORD BLOOD,STEM-CELLS,BONE-MARROW,PERIPHERAL-BLOOD,ADIPOSE-TISSUE,ADULT,DIFFERENTIATION,REGENERATION,EXPRESSION},
  language     = {eng},
  pages        = {13},
  title        = {Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources},
  url          = {http://dx.doi.org/10.1186/scrt395},
  volume       = {5},
  year         = {2014},
}

Chicago
De Schauwer, Catharina, Karen Goossens, Sofie Piepers, Maarten Hoogewijs, Jan Govaere, Katrien Smits, Evelyne Meyer, Ann Van Soom, and Gerlinde Van de Walle. 2014. “Characterization and Profiling of Immunomodulatory Genes of Equine Mesenchymal Stromal Cells from Non-invasive Sources.” Stem Cell Research & Therapy 5.
APA
De Schauwer, C., Goossens, K., Piepers, S., Hoogewijs, M., Govaere, J., Smits, K., Meyer, E., et al. (2014). Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources. STEM CELL RESEARCH & THERAPY, 5.
Vancouver
1.
De Schauwer C, Goossens K, Piepers S, Hoogewijs M, Govaere J, Smits K, et al. Characterization and profiling of immunomodulatory genes of equine mesenchymal stromal cells from non-invasive sources. STEM CELL RESEARCH & THERAPY. 2014;5.
MLA
De Schauwer, Catharina, Karen Goossens, Sofie Piepers, et al. “Characterization and Profiling of Immunomodulatory Genes of Equine Mesenchymal Stromal Cells from Non-invasive Sources.” STEM CELL RESEARCH & THERAPY 5 (2014): n. pag. Print.