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Comparative performances of HIV-1 RNA load assays at low viral load levels: results of an international collaboration

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Abstract
Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of similar to 100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.
Keywords
AMPLICOR ASSAY, VERSION 1.5, DRUG-RESISTANCE, TAQMAN(R) HIV-1, VIROLOGICAL FAILURE, ANTIRETROVIRAL THERAPY, PCR ASSAY, :IMMUNODEFICIENCY-VIRUS TYPE-1, NON-B SUBTYPES, ABBOTT REALTIME

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Chicago
Swenson, Luke C, Bryan Cobb, Anna Maria Geretti, P Richard Harrigan, Mario Poljak, Carole Seguin-Devaux, Chris Verhofstede, et al. 2014. “Comparative Performances of HIV-1 RNA Load Assays at Low Viral Load Levels: Results of an International Collaboration.” Journal of Clinical Microbiology 52 (2): 517–523.
APA
Swenson, L. C., Cobb, B., Geretti, A. M., Harrigan, P. R., Poljak, M., Seguin-Devaux, C., Verhofstede, C., et al. (2014). Comparative performances of HIV-1 RNA load assays at low viral load levels: results of an international collaboration. JOURNAL OF CLINICAL MICROBIOLOGY, 52(2), 517–523.
Vancouver
1.
Swenson LC, Cobb B, Geretti AM, Harrigan PR, Poljak M, Seguin-Devaux C, et al. Comparative performances of HIV-1 RNA load assays at low viral load levels: results of an international collaboration. JOURNAL OF CLINICAL MICROBIOLOGY. 2014;52(2):517–23.
MLA
Swenson, Luke C, Bryan Cobb, Anna Maria Geretti, et al. “Comparative Performances of HIV-1 RNA Load Assays at Low Viral Load Levels: Results of an International Collaboration.” JOURNAL OF CLINICAL MICROBIOLOGY 52.2 (2014): 517–523. Print.
@article{4351431,
  abstract     = {Low-level viremia during antiretroviral therapy and its accurate measurement are increasingly relevant. Here, we present an international collaboration of 4,221 paired blood plasma viral load (pVL) results from four commercial assays, emphasizing the data with low pVL. The assays compared were the Abbott RealTime assay, the Roche Amplicor assay, and the Roche TaqMan version 1 and version 2 assays. The correlation between the assays was 0.90 to 0.97. However, at a low pVL, the correlation fell to 0.45 to 0.85. The observed interassay concordance was higher when detectability was defined as 200 copies/ml than when it was defined as 50 copies/ml. A pVL of similar to 100 to 125 copies/ml by the TaqMan version 1 and version 2 assays corresponded best to a 50-copies/ml threshold with the Amplicor assay. Correlation and concordance between the viral load assays were lower at a low pVL. Clear guidelines are needed on the clinical significance of low-level viremia.},
  author       = {Swenson, Luke C and Cobb, Bryan and Geretti, Anna Maria and Harrigan, P Richard and Poljak, Mario and Seguin-Devaux, Carole and Verhofstede, Chris and Wirden, Marc and Amendola, Alessandra and Boni, Jurg and Bourlet, Thomas and Huder, Jon B and Karasi, Jean-Claude and Lepej, Snjezana Zidovec and Lunar, Maja M and Mukabayire, Odette and Schuurman, Rob and Toma\v{z}i\v{c}, Janez and Van Laethem, Kristel and Vandekerckhove, Linos and Wensing, Annemarie MJ},
  issn         = {0095-1137},
  journal      = {JOURNAL OF CLINICAL MICROBIOLOGY},
  language     = {eng},
  number       = {2},
  pages        = {517--523},
  title        = {Comparative performances of HIV-1 RNA load assays at low viral load levels: results of an international collaboration},
  url          = {http://dx.doi.org/10.1128/JCM.02461-13},
  volume       = {52},
  year         = {2014},
}

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