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The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants

(2014) CELL. 156(4). p.691-704
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HPC-UGent: the central High Performance Computing infrastructure of Ghent University
Abstract
Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.
Keywords
YEAST, ARABIDOPSIS, TRAFFICKING, DYNAMIN, ORGAN DEVELOPMENT, SOMATIC CYTOKINESIS, DOMAIN-CONTAINING PROTEINS, PLASMA-MEMBRANE, DEPENDENT ENDOCYTOSIS, AUXIN EFFLUX

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MLA
Gadeyne, Astrid, Clara Sánchez-Rodríguez, Steffen Vanneste, et al. “The TPLATE Adaptor Complex Drives Clathrin-mediated Endocytosis in Plants.” CELL 156.4 (2014): 691–704. Print.
APA
Gadeyne, A., Sánchez-Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H., Vanneste, K., Van Leene, J., et al. (2014). The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. CELL, 156(4), 691–704.
Chicago author-date
Gadeyne, Astrid, Clara Sánchez-Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. 2014. “The TPLATE Adaptor Complex Drives Clathrin-mediated Endocytosis in Plants.” Cell 156 (4): 691–704.
Chicago author-date (all authors)
Gadeyne, Astrid, Clara Sánchez-Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, Nancy De Winne, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Bernard Cannoot, Leen Vercruysse, Jonathan R Mayers, Maciej Adamowski, Urszula Kania, Matthias Ehrlich, Alois Schweighofer, Tijs Ketelaar, Steven Maere, Sebastian Y Bednarek, Jiri Friml, Kris Gevaert, Erwin Witters, Eugenia Russinova, Staffan Persson, Geert De Jaeger, and Daniël Van Damme. 2014. “The TPLATE Adaptor Complex Drives Clathrin-mediated Endocytosis in Plants.” Cell 156 (4): 691–704.
Vancouver
1.
Gadeyne A, Sánchez-Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste K, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. CELL. 2014;156(4):691–704.
IEEE
[1]
A. Gadeyne et al., “The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants,” CELL, vol. 156, no. 4, pp. 691–704, 2014.
@article{4349685,
  abstract     = {Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis.},
  author       = {Gadeyne, Astrid and Sánchez-Rodríguez, Clara and Vanneste, Steffen and Di Rubbo, Simone and Zauber, Henrik and Vanneste, Kevin and Van Leene, Jelle and De Winne, Nancy and Eeckhout, Dominique and Persiau, Geert and Van De Slijke, Eveline and Cannoot, Bernard and Vercruysse, Leen and Mayers, Jonathan R and Adamowski, Maciej and Kania, Urszula and Ehrlich, Matthias and Schweighofer, Alois and Ketelaar, Tijs and Maere, Steven and Bednarek, Sebastian Y and Friml, Jiri and Gevaert, Kris and Witters, Erwin and Russinova, Eugenia and Persson, Staffan and De Jaeger, Geert and Van Damme, Daniël},
  issn         = {0092-8674},
  journal      = {CELL},
  keywords     = {YEAST,ARABIDOPSIS,TRAFFICKING,DYNAMIN,ORGAN DEVELOPMENT,SOMATIC CYTOKINESIS,DOMAIN-CONTAINING PROTEINS,PLASMA-MEMBRANE,DEPENDENT ENDOCYTOSIS,AUXIN EFFLUX},
  language     = {eng},
  number       = {4},
  pages        = {691--704},
  title        = {The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants},
  url          = {http://dx.doi.org/10.1016/j.cell.2014.01.039},
  volume       = {156},
  year         = {2014},
}

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