Ghent University Academic Bibliography

Advanced

Defining the selectivity of processes along the auxin response chain: a study using auxin analogues

Sibu Simon UGent, Martin Kubeš, Pawel Radoslaw Baster, Stephanie Robert UGent, Petre Ivanov Dobrev, Jiri Friml, Jan Petrášek and Eva Zažímalová (2013) NEW PHYTOLOGIST. 200(4). p.1034-1048
abstract
The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
2, ARABIDOPSIS-THALIANA, BOX PROTEIN TIR1, 2_4-dichlorophenoxyacetic acid (2_4-D), naphthalene-1-acetic acid (NAA), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), auxin transport, auxin signalling, auxin analogues, 4-DICHLOROPHENOXYACETIC ACID, PRECURSOR INDOLE-3-BUTYRIC ACID, TOBACCO CELLS, ROOT GRAVITROPISM, PLANT-GROWTH, TRANSPORT, EFFLUX, RECEPTOR
journal title
NEW PHYTOLOGIST
New Phytol.
volume
200
issue
4
pages
1034 - 1048
Web of Science type
Article
Web of Science id
000330955300012
JCR category
PLANT SCIENCES
JCR impact factor
6.545 (2013)
JCR rank
9/199 (2013)
JCR quartile
1 (2013)
ISSN
1469-8137
DOI
10.1111/nph.12437
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4349381
handle
http://hdl.handle.net/1854/LU-4349381
date created
2014-04-01 13:19:30
date last changed
2016-12-19 15:45:49
@article{4349381,
  abstract     = {The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. 
Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. 
This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. 
The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery.},
  author       = {Simon, Sibu and Kube\v{s}, Martin and Baster, Pawel Radoslaw and Robert, Stephanie and Dobrev, Petre Ivanov and Friml, Jiri and Petr{\'a}\v{s}ek, Jan and Za\v{z}{\'i}malov{\'a}, Eva},
  issn         = {1469-8137},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {2,ARABIDOPSIS-THALIANA,BOX PROTEIN TIR1,2\_4-dichlorophenoxyacetic acid (2\_4-D),naphthalene-1-acetic acid (NAA),indole-3-butyric acid (IBA),indole-3-acetic acid (IAA),auxin transport,auxin signalling,auxin analogues,4-DICHLOROPHENOXYACETIC ACID,PRECURSOR INDOLE-3-BUTYRIC ACID,TOBACCO CELLS,ROOT GRAVITROPISM,PLANT-GROWTH,TRANSPORT,EFFLUX,RECEPTOR},
  language     = {eng},
  number       = {4},
  pages        = {1034--1048},
  title        = {Defining the selectivity of processes along the auxin response chain: a study using auxin analogues},
  url          = {http://dx.doi.org/10.1111/nph.12437},
  volume       = {200},
  year         = {2013},
}

Chicago
Simon, Sibu, Martin Kubeš, Pawel Radoslaw Baster, Stephanie Robert, Petre Ivanov Dobrev, Jiri Friml, Jan Petrášek, and Eva Zažímalová. 2013. “Defining the Selectivity of Processes Along the Auxin Response Chain: a Study Using Auxin Analogues.” New Phytologist 200 (4): 1034–1048.
APA
Simon, Sibu, Kubeš, M., Baster, P. R., Robert, S., Dobrev, P. I., Friml, J., Petrášek, J., et al. (2013). Defining the selectivity of processes along the auxin response chain: a study using auxin analogues. NEW PHYTOLOGIST, 200(4), 1034–1048.
Vancouver
1.
Simon S, Kubeš M, Baster PR, Robert S, Dobrev PI, Friml J, et al. Defining the selectivity of processes along the auxin response chain: a study using auxin analogues. NEW PHYTOLOGIST. 2013;200(4):1034–48.
MLA
Simon, Sibu, Martin Kubeš, Pawel Radoslaw Baster, et al. “Defining the Selectivity of Processes Along the Auxin Response Chain: a Study Using Auxin Analogues.” NEW PHYTOLOGIST 200.4 (2013): 1034–1048. Print.