Gluconobacter cerevisiae sp. nov., isolated from the brewery environment
- Author
- Freek Spitaels (UGent) , Anneleen Wieme (UGent) , Tom Balzarini, Ilse Cleenwerck (UGent) , Anita Van Landschoot (UGent) , Luc De Vuyst and Peter Vandamme (UGent)
- Organization
- Abstract
- Three strains, LMG 27748(T), LMG 27749 and LMG 27882 with identical MALDI-TOF mass spectra were isolated from samples taken from the brewery environment. Analysis of the 16S rRNA gene sequence of strain LMG 27748(T) revealed that the taxon it represents was closely related to type strains of the species Gluconobacter albidus (100% sequence similarity), Gluconobacter kondonii (99.9 %), Gluconobacter sphaericus (99.9 %) and Gluconobacter kanchanaburiensis (99.5 %). DNA DNA hybridization experiments on the type strains of these species revealed moderate DNA relatedness values (39-65%). The three strains used D-fructose, D-sorbitol, meso-erythritol, glycerol,L-sorbose, ethanol (weakly), sucrose and raffinose as a sole carbon source for growth (weak growth on the latter two carbon sources was obtained for strains LMG 27748(T) and LMG 27882). The strains were unable to grow on glucose-yeast extract medium at 37 degrees C. They produced acid from meso-erythritol and sucrose, but not from raffinose. D-Gluconic acid, 2-keto-D-gluconic acid and 5-keto-D-gluconic acid were produced from D-glucose, but not 2,5-diketo-D-gluconic acid. These genotypic and phenotypic characteristics distinguish strains LMG 27748(T), LMG 27749 and LMG 27882 from species of the genus Gluconobacter with validly published names and, therefore, we propose classifying them formally as representatives of a novel species, Gluconobacter cerevisiae sp. nov., with LMG 27748(T) (=DSM 27644(T)) as the type strain.
- Keywords
- ALPHA-PROTEOBACTERIA, ACETIC-ACID BACTERIUM, BIOTECHNOLOGICAL APPLICATIONS, GENUS GLUCONOBACTER, SEQUENCE, STRAINS, ACETOBACTER, IDENTIFICATION, ALPHAPROTEOBACTERIA, GLUCONACETOBACTER
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-4344474
- MLA
- Spitaels, Freek, et al. “Gluconobacter Cerevisiae Sp. Nov., Isolated from the Brewery Environment.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 4, 2014, pp. 1134–41, doi:10.1099/ijs.0.059311-0.
- APA
- Spitaels, F., Wieme, A., Balzarini, T., Cleenwerck, I., Van Landschoot, A., De Vuyst, L., & Vandamme, P. (2014). Gluconobacter cerevisiae sp. nov., isolated from the brewery environment. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(4), 1134–1141. https://doi.org/10.1099/ijs.0.059311-0
- Chicago author-date
- Spitaels, Freek, Anneleen Wieme, Tom Balzarini, Ilse Cleenwerck, Anita Van Landschoot, Luc De Vuyst, and Peter Vandamme. 2014. “Gluconobacter Cerevisiae Sp. Nov., Isolated from the Brewery Environment.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64 (4): 1134–41. https://doi.org/10.1099/ijs.0.059311-0.
- Chicago author-date (all authors)
- Spitaels, Freek, Anneleen Wieme, Tom Balzarini, Ilse Cleenwerck, Anita Van Landschoot, Luc De Vuyst, and Peter Vandamme. 2014. “Gluconobacter Cerevisiae Sp. Nov., Isolated from the Brewery Environment.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64 (4): 1134–1141. doi:10.1099/ijs.0.059311-0.
- Vancouver
- 1.Spitaels F, Wieme A, Balzarini T, Cleenwerck I, Van Landschoot A, De Vuyst L, et al. Gluconobacter cerevisiae sp. nov., isolated from the brewery environment. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(4):1134–41.
- IEEE
- [1]F. Spitaels et al., “Gluconobacter cerevisiae sp. nov., isolated from the brewery environment,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 4, pp. 1134–1141, 2014.
@article{4344474,
abstract = {{Three strains, LMG 27748(T), LMG 27749 and LMG 27882 with identical MALDI-TOF mass spectra were isolated from samples taken from the brewery environment. Analysis of the 16S rRNA gene sequence of strain LMG 27748(T) revealed that the taxon it represents was closely related to type strains of the species Gluconobacter albidus (100% sequence similarity), Gluconobacter kondonii (99.9 %), Gluconobacter sphaericus (99.9 %) and Gluconobacter kanchanaburiensis (99.5 %). DNA DNA hybridization experiments on the type strains of these species revealed moderate DNA relatedness values (39-65%). The three strains used D-fructose, D-sorbitol, meso-erythritol, glycerol,L-sorbose, ethanol (weakly), sucrose and raffinose as a sole carbon source for growth (weak growth on the latter two carbon sources was obtained for strains LMG 27748(T) and LMG 27882). The strains were unable to grow on glucose-yeast extract medium at 37 degrees C. They produced acid from meso-erythritol and sucrose, but not from raffinose. D-Gluconic acid, 2-keto-D-gluconic acid and 5-keto-D-gluconic acid were produced from D-glucose, but not 2,5-diketo-D-gluconic acid. These genotypic and phenotypic characteristics distinguish strains LMG 27748(T), LMG 27749 and LMG 27882 from species of the genus Gluconobacter with validly published names and, therefore, we propose classifying them formally as representatives of a novel species, Gluconobacter cerevisiae sp. nov., with LMG 27748(T) (=DSM 27644(T)) as the type strain.}},
author = {{Spitaels, Freek and Wieme, Anneleen and Balzarini, Tom and Cleenwerck, Ilse and Van Landschoot, Anita and De Vuyst, Luc and Vandamme, Peter}},
issn = {{1466-5026}},
journal = {{INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}},
keywords = {{ALPHA-PROTEOBACTERIA,ACETIC-ACID BACTERIUM,BIOTECHNOLOGICAL APPLICATIONS,GENUS GLUCONOBACTER,SEQUENCE,STRAINS,ACETOBACTER,IDENTIFICATION,ALPHAPROTEOBACTERIA,GLUCONACETOBACTER}},
language = {{eng}},
number = {{4}},
pages = {{1134--1141}},
title = {{Gluconobacter cerevisiae sp. nov., isolated from the brewery environment}},
url = {{http://doi.org/10.1099/ijs.0.059311-0}},
volume = {{64}},
year = {{2014}},
}
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