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Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner

(2014) PLANT PHYSIOLOGY. 165(1). p.290-308
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
To study the effect of short N-acetylglucosamine (GlcNAc) oligosaccharides on the physiology of plants, N-ACETYLGLUCOSAMINYLTRANSFERASE (NodC) of Azorhizobium caulinodans was expressed in Arabidopsis (Arabidopsis thaliana). The corresponding enzyme catalyzes the polymerization of GlcNAc and, accordingly, beta-1,4-GlcNAc oligomers accumulated in the plant. A phenotype characterized by difficulties in developing an inflorescence stem was visible when plants were grown for several weeks under short-day conditions before transfer to long-day conditions. In addition, a positive correlation between the oligomer concentration and the penetrance of the phenotype was demonstrated. Although NodC overexpression lines produced less cell wall compared with wildtype plants under nonpermissive conditions, no indications were found for changes in the amount of the major cell wall polymers. The effect on the cell wall was reflected at the transcriptome level. In addition to genes encoding cell wall-modifying enzymes, a whole set of genes encoding membrane- coupled receptor-like kinases were differentially expressed upon GlcNAc accumulation, many of which encoded proteins with an extracellular Domain of Unknown Function26. Although stress-related genes were also differentially expressed, the observed response differed from that of a classical chitin response. This is in line with the fact that the produced chitin oligomers were too small to activate the chitin receptor-mediated signal cascade. Based on our observations, we propose a model in which the oligosaccharides modify the architecture of the cell wall by acting as competitors in carbohydrate-carbohydrate or carbohydrate-protein interactions, thereby affecting noncovalent interactions in the cell wall or at the interface between the cell wall and the plasma membrane.
Keywords
CHITIN, CELLULOSE, PROTEIN-KINASES, ECTOPIC DEPOSITION, ACETOBACTER-XYLINUM, TRANSCRIPTION FACTOR, ARABIDOPSIS-THALIANA, RECEPTOR-LIKE KINASE, LIGNIN BIOSYNTHESIS PERTURBATIONS, WHEAT-GERM-AGGLUTININ

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Citation

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Chicago
Vanholme, Bartel, Ruben Vanholme, Halbay Turumtay, Geert Goeminne, Igor Cesarino, Florence Goubet, Kris Morreel, et al. 2014. “Accumulation of N-acetylglucosamine Oligomers in the Plant Cell Wall Affects Plant Architecture in a Dose-dependent and Conditional Manner.” Plant Physiology 165 (1): 290–308.
APA
Vanholme, B., Vanholme, R., Turumtay, H., Goeminne, G., Cesarino, I., Goubet, F., Morreel, K., et al. (2014). Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner. PLANT PHYSIOLOGY, 165(1), 290–308.
Vancouver
1.
Vanholme B, Vanholme R, Turumtay H, Goeminne G, Cesarino I, Goubet F, et al. Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner. PLANT PHYSIOLOGY. 2014;165(1):290–308.
MLA
Vanholme, Bartel, Ruben Vanholme, Halbay Turumtay, et al. “Accumulation of N-acetylglucosamine Oligomers in the Plant Cell Wall Affects Plant Architecture in a Dose-dependent and Conditional Manner.” PLANT PHYSIOLOGY 165.1 (2014): 290–308. Print.
@article{4344106,
  abstract     = {To study the effect of short N-acetylglucosamine (GlcNAc) oligosaccharides on the physiology of plants, N-ACETYLGLUCOSAMINYLTRANSFERASE (NodC) of Azorhizobium caulinodans was expressed in Arabidopsis (Arabidopsis thaliana). The corresponding enzyme catalyzes the polymerization of GlcNAc and, accordingly, beta-1,4-GlcNAc oligomers accumulated in the plant. A phenotype characterized by difficulties in developing an inflorescence stem was visible when plants were grown for several weeks under short-day conditions before transfer to long-day conditions. In addition, a positive correlation between the oligomer concentration and the penetrance of the phenotype was demonstrated. Although NodC overexpression lines produced less cell wall compared with wildtype plants under nonpermissive conditions, no indications were found for changes in the amount of the major cell wall polymers. The effect on the cell wall was reflected at the transcriptome level. In addition to genes encoding cell wall-modifying enzymes, a whole set of genes encoding membrane- coupled receptor-like kinases were differentially expressed upon GlcNAc accumulation, many of which encoded proteins with an extracellular Domain of Unknown Function26. Although stress-related genes were also differentially expressed, the observed response differed from that of a classical chitin response. This is in line with the fact that the produced chitin oligomers were too small to activate the chitin receptor-mediated signal cascade. Based on our observations, we propose a model in which the oligosaccharides modify the architecture of the cell wall by acting as competitors in carbohydrate-carbohydrate or carbohydrate-protein interactions, thereby affecting noncovalent interactions in the cell wall or at the interface between the cell wall and the plasma membrane.},
  author       = {Vanholme, Bartel and Vanholme, Ruben and Turumtay, Halbay and Goeminne, Geert and Cesarino, Igor and Goubet, Florence and Morreel, Kris and Rencoret, Jorge and Bulone, Vincent and Hooijmaijers, Cortwa and De Rycke, Riet and Gheysen, Godelieve and Ralph, John and De Block, Marc  and Meulewaeter, Frank and Boerjan, Wout},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {CHITIN,CELLULOSE,PROTEIN-KINASES,ECTOPIC DEPOSITION,ACETOBACTER-XYLINUM,TRANSCRIPTION FACTOR,ARABIDOPSIS-THALIANA,RECEPTOR-LIKE KINASE,LIGNIN BIOSYNTHESIS PERTURBATIONS,WHEAT-GERM-AGGLUTININ},
  language     = {eng},
  number       = {1},
  pages        = {290--308},
  title        = {Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner},
  url          = {http://dx.doi.org/10.1104/pp.113.233742},
  volume       = {165},
  year         = {2014},
}

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