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Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance

(2014) BIOLOGY AND FERTILITY OF SOILS. 50(3). p.517-525
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Abstract
Quantifying biologically fixed nitrogen (BNF) by legumes through the N-15 natural abundance techniques requires correct determination of a so-called B value. We hypothesized that significant variations in B values exist between faba bean (Vicia faba L.) varieties having consequences for BNF and N balance calculations. We experimentally determined B values for a range of faba bean varieties and quantified to what extent variety has an effect on B values and hence BNF quantification. Seeds of six faba bean varieties released in Ethiopia were inoculated with Rhizobium fabae strain LMG 23997-19 and grown in vermiculite with an N-free nutrient solution in a growth room until full flowering. Total N and N-15 content of nodules, roots, and shoot components was analyzed separately to determine the weighted whole plant N-15 fractionation during N-2 fixation, i.e., the B value. Owing to its large seed size and high N content, a correction for seed N was carried out. We then calculated the percentage of N derived from air (%Ndfa), BNF, and N balance for faba beans grown in the field using three B value scenarios (variety specific B value corrected for seed N, variety specific B value without seed N correction, and a literature derived B value). Whole plant seed N corrected B values were significantly different (P < 0.05) between varieties and varied between +0.5 +/- 0.4 and -1.9 +/- 1.4aEuro degrees suggesting a variable isotope fractionation during N-2 fixation. The %Ndfa was significantly (P < 0.05) different between varieties (59 +/- 4.2-84 +/- 4.5 %) using seed N corrected B values. BNF (218 +/- 26.2-362 +/- 34.7 kg N ha(-1)) was significantly (P < 0.05) different between varieties for corrected and uncorrected B values. Soil N balance did not result in statistically significant (P > 0.05) difference between varieties for all three B value scenarios. Use of inappropriate B values masked the difference between varieties and affected their ranking in terms of BNF, resulting from an over- to underestimation of 15 and 19 %, respectively. When applying the N-15 natural abundance technique to compare BNF of legume accessions, we recommend determining a B value for each accession. For legumes with large seeds such as faba beans, it is moreover essential to account for seed N when determining the B value.
Keywords
INOCULATION, Faba bean, FERTILIZATION, CROP, FIELD, PLANT ECOLOGY, AGRICULTURAL SYSTEMS, PASTURE LEGUMES, ISOTOPIC FRACTIONATION, NITROGEN-FIXATION, SOUTH-WEST AUSTRALIA, N-15 natural abundance, B value

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MLA
Woldekirstos, Amsalu Nebiyu, Dries Huygens, Hari Ram Upadhayay, et al. “Importance of Correct B Value Determination to Quantify Biological N2 Fixation and N Balances of Faba Beans (Vicia Faba L.) via 15N Natural Abundance.” BIOLOGY AND FERTILITY OF SOILS 50.3 (2014): 517–525. Print.
APA
Woldekirstos, A. N., Huygens, D., Upadhayay, H. R., Diels, J., & Boeckx, P. (2014). Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance. BIOLOGY AND FERTILITY OF SOILS, 50(3), 517–525.
Chicago author-date
Woldekirstos, Amsalu Nebiyu, Dries Huygens, Hari Ram Upadhayay, Jan Diels, and Pascal Boeckx. 2014. “Importance of Correct B Value Determination to Quantify Biological N2 Fixation and N Balances of Faba Beans (Vicia Faba L.) via 15N Natural Abundance.” Biology and Fertility of Soils 50 (3): 517–525.
Chicago author-date (all authors)
Woldekirstos, Amsalu Nebiyu, Dries Huygens, Hari Ram Upadhayay, Jan Diels, and Pascal Boeckx. 2014. “Importance of Correct B Value Determination to Quantify Biological N2 Fixation and N Balances of Faba Beans (Vicia Faba L.) via 15N Natural Abundance.” Biology and Fertility of Soils 50 (3): 517–525.
Vancouver
1.
Woldekirstos AN, Huygens D, Upadhayay HR, Diels J, Boeckx P. Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance. BIOLOGY AND FERTILITY OF SOILS. 2014;50(3):517–25.
IEEE
[1]
A. N. Woldekirstos, D. Huygens, H. R. Upadhayay, J. Diels, and P. Boeckx, “Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance,” BIOLOGY AND FERTILITY OF SOILS, vol. 50, no. 3, pp. 517–525, 2014.
@article{4337829,
  abstract     = {Quantifying biologically fixed nitrogen (BNF) by legumes through the N-15 natural abundance techniques requires correct determination of a so-called B value. We hypothesized that significant variations in B values exist between faba bean (Vicia faba L.) varieties having consequences for BNF and N balance calculations. We experimentally determined B values for a range of faba bean varieties and quantified to what extent variety has an effect on B values and hence BNF quantification. Seeds of six faba bean varieties released in Ethiopia were inoculated with Rhizobium fabae strain LMG 23997-19 and grown in vermiculite with an N-free nutrient solution in a growth room until full flowering. Total N and N-15 content of nodules, roots, and shoot components was analyzed separately to determine the weighted whole plant N-15 fractionation during N-2 fixation, i.e., the B value. Owing to its large seed size and high N content, a correction for seed N was carried out. We then calculated the percentage of N derived from air (%Ndfa), BNF, and N balance for faba beans grown in the field using three B value scenarios (variety specific B value corrected for seed N, variety specific B value without seed N correction, and a literature derived B value). Whole plant seed N corrected B values were significantly different (P < 0.05) between varieties and varied between +0.5 +/- 0.4 and -1.9 +/- 1.4aEuro degrees suggesting a variable isotope fractionation during N-2 fixation. The %Ndfa was significantly (P < 0.05) different between varieties (59 +/- 4.2-84 +/- 4.5 %) using seed N corrected B values. BNF (218 +/- 26.2-362 +/- 34.7 kg N ha(-1)) was significantly (P < 0.05) different between varieties for corrected and uncorrected B values. Soil N balance did not result in statistically significant (P > 0.05) difference between varieties for all three B value scenarios. Use of inappropriate B values masked the difference between varieties and affected their ranking in terms of BNF, resulting from an over- to underestimation of 15 and 19 %, respectively. When applying the N-15 natural abundance technique to compare BNF of legume accessions, we recommend determining a B value for each accession. For legumes with large seeds such as faba beans, it is moreover essential to account for seed N when determining the B value.},
  author       = {Woldekirstos, Amsalu Nebiyu and Huygens, Dries and Upadhayay, Hari Ram and Diels, Jan and Boeckx, Pascal},
  issn         = {0178-2762},
  journal      = {BIOLOGY AND FERTILITY OF SOILS},
  keywords     = {INOCULATION,Faba bean,FERTILIZATION,CROP,FIELD,PLANT ECOLOGY,AGRICULTURAL SYSTEMS,PASTURE LEGUMES,ISOTOPIC FRACTIONATION,NITROGEN-FIXATION,SOUTH-WEST AUSTRALIA,N-15 natural abundance,B value},
  language     = {eng},
  number       = {3},
  pages        = {517--525},
  title        = {Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance},
  url          = {http://dx.doi.org/10.1007/s00374-013-0874-7},
  volume       = {50},
  year         = {2014},
}

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