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Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance

(2014) BIOLOGY AND FERTILITY OF SOILS. 50(3). p.517-525
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Abstract
Quantifying biologically fixed nitrogen (BNF) by legumes through the N-15 natural abundance techniques requires correct determination of a so-called B value. We hypothesized that significant variations in B values exist between faba bean (Vicia faba L.) varieties having consequences for BNF and N balance calculations. We experimentally determined B values for a range of faba bean varieties and quantified to what extent variety has an effect on B values and hence BNF quantification. Seeds of six faba bean varieties released in Ethiopia were inoculated with Rhizobium fabae strain LMG 23997-19 and grown in vermiculite with an N-free nutrient solution in a growth room until full flowering. Total N and N-15 content of nodules, roots, and shoot components was analyzed separately to determine the weighted whole plant N-15 fractionation during N-2 fixation, i.e., the B value. Owing to its large seed size and high N content, a correction for seed N was carried out. We then calculated the percentage of N derived from air (%Ndfa), BNF, and N balance for faba beans grown in the field using three B value scenarios (variety specific B value corrected for seed N, variety specific B value without seed N correction, and a literature derived B value). Whole plant seed N corrected B values were significantly different (P < 0.05) between varieties and varied between +0.5 +/- 0.4 and -1.9 +/- 1.4aEuro degrees suggesting a variable isotope fractionation during N-2 fixation. The %Ndfa was significantly (P < 0.05) different between varieties (59 +/- 4.2-84 +/- 4.5 %) using seed N corrected B values. BNF (218 +/- 26.2-362 +/- 34.7 kg N ha(-1)) was significantly (P < 0.05) different between varieties for corrected and uncorrected B values. Soil N balance did not result in statistically significant (P > 0.05) difference between varieties for all three B value scenarios. Use of inappropriate B values masked the difference between varieties and affected their ranking in terms of BNF, resulting from an over- to underestimation of 15 and 19 %, respectively. When applying the N-15 natural abundance technique to compare BNF of legume accessions, we recommend determining a B value for each accession. For legumes with large seeds such as faba beans, it is moreover essential to account for seed N when determining the B value.
Keywords
INOCULATION, Faba bean, FERTILIZATION, CROP, FIELD, PLANT ECOLOGY, AGRICULTURAL SYSTEMS, PASTURE LEGUMES, ISOTOPIC FRACTIONATION, NITROGEN-FIXATION, SOUTH-WEST AUSTRALIA, N-15 natural abundance, B value

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Chicago
Woldekirstos, Amsalu Nebiyu, Dries Huygens, Hari Ram Upadhayay, Jan Diels, and Pascal Boeckx. 2014. “Importance of Correct B Value Determination to Quantify Biological N2 Fixation and N Balances of Faba Beans (Vicia Faba L.) via 15N Natural Abundance.” Biology and Fertility of Soils 50 (3): 517–525.
APA
Woldekirstos, A. N., Huygens, D., Upadhayay, H. R., Diels, J., & Boeckx, P. (2014). Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance. BIOLOGY AND FERTILITY OF SOILS, 50(3), 517–525.
Vancouver
1.
Woldekirstos AN, Huygens D, Upadhayay HR, Diels J, Boeckx P. Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance. BIOLOGY AND FERTILITY OF SOILS. 2014;50(3):517–25.
MLA
Woldekirstos, Amsalu Nebiyu, Dries Huygens, Hari Ram Upadhayay, et al. “Importance of Correct B Value Determination to Quantify Biological N2 Fixation and N Balances of Faba Beans (Vicia Faba L.) via 15N Natural Abundance.” BIOLOGY AND FERTILITY OF SOILS 50.3 (2014): 517–525. Print.
@article{4337829,
  abstract     = {Quantifying biologically fixed nitrogen (BNF) by legumes through the N-15 natural abundance techniques requires correct determination of a so-called B value. We hypothesized that significant variations in B values exist between faba bean (Vicia faba L.) varieties having consequences for BNF and N balance calculations. We experimentally determined B values for a range of faba bean varieties and quantified to what extent variety has an effect on B values and hence BNF quantification. Seeds of six faba bean varieties released in Ethiopia were inoculated with Rhizobium fabae strain LMG 23997-19 and grown in vermiculite with an N-free nutrient solution in a growth room until full flowering. Total N and N-15 content of nodules, roots, and shoot components was analyzed separately to determine the weighted whole plant N-15 fractionation during N-2 fixation, i.e., the B value. Owing to its large seed size and high N content, a correction for seed N was carried out. We then calculated the percentage of N derived from air (\%Ndfa), BNF, and N balance for faba beans grown in the field using three B value scenarios (variety specific B value corrected for seed N, variety specific B value without seed N correction, and a literature derived B value). Whole plant seed N corrected B values were significantly different (P {\textlangle} 0.05) between varieties and varied between +0.5 +/- 0.4 and -1.9 +/- 1.4aEuro degrees suggesting a variable isotope fractionation during N-2 fixation. The \%Ndfa was significantly (P {\textlangle} 0.05) different between varieties (59 +/- 4.2-84 +/- 4.5 \%) using seed N corrected B values. BNF (218 +/- 26.2-362 +/- 34.7 kg N ha(-1)) was significantly (P {\textlangle} 0.05) different between varieties for corrected and uncorrected B values. Soil N balance did not result in statistically significant (P {\textrangle} 0.05) difference between varieties for all three B value scenarios. Use of inappropriate B values masked the difference between varieties and affected their ranking in terms of BNF, resulting from an over- to underestimation of 15 and 19 \%, respectively. When applying the N-15 natural abundance technique to compare BNF of legume accessions, we recommend determining a B value for each accession. For legumes with large seeds such as faba beans, it is moreover essential to account for seed N when determining the B value.},
  author       = {Woldekirstos, Amsalu Nebiyu and Huygens, Dries and Upadhayay, Hari Ram and Diels, Jan and Boeckx, Pascal},
  issn         = {0178-2762},
  journal      = {BIOLOGY AND FERTILITY OF SOILS},
  keyword      = {INOCULATION,Faba bean,FERTILIZATION,CROP,FIELD,PLANT ECOLOGY,AGRICULTURAL SYSTEMS,PASTURE LEGUMES,ISOTOPIC FRACTIONATION,NITROGEN-FIXATION,SOUTH-WEST AUSTRALIA,N-15 natural abundance,B value},
  language     = {eng},
  number       = {3},
  pages        = {517--525},
  title        = {Importance of correct B value determination to quantify biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance},
  url          = {http://dx.doi.org/10.1007/s00374-013-0874-7},
  volume       = {50},
  year         = {2014},
}

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