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A protein domain-based interactome network for C. elegans early embryogenesis

(2008) CELL. 134(3). p.534-545
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Abstract
Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome'' networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in amore complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
Keywords
INTERACTION MAP, GENOME, 2-HYBRID SCREENS, CENTROSOME MATURATION, SACCHAROMYCES-CEREVISIAE, NUCLEAR-PORE COMPLEX, CAENORHABDITIS-ELEGANS, IDENTIFICATION, RESOURCE, SCALE

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Citation

Please use this url to cite or link to this publication:

Chicago
Boxem, Mike, Zoltan Maliga, Niels Klitgord, Na Li, Irma Lemmens, Miyeko Mana, Lorenzo de Lichtervelde, et al. 2008. “A Protein Domain-based Interactome Network for C. Elegans Early Embryogenesis.” Cell 134 (3): 534–545.
APA
Boxem, M., Maliga, Z., Klitgord, N., Li, N., Lemmens, I., Mana, M., de Lichtervelde, L., et al. (2008). A protein domain-based interactome network for C. elegans early embryogenesis. CELL, 134(3), 534–545.
Vancouver
1.
Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, et al. A protein domain-based interactome network for C. elegans early embryogenesis. CELL. 2008;134(3):534–45.
MLA
Boxem, Mike, Zoltan Maliga, Niels Klitgord, et al. “A Protein Domain-based Interactome Network for C. Elegans Early Embryogenesis.” CELL 134.3 (2008): 534–545. Print.
@article{433014,
  abstract     = {Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or {\textacutedbl}interactome'' networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in amore complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.},
  author       = {Boxem, Mike and Maliga, Zoltan and Klitgord, Niels and Li, Na and Lemmens, Irma and Mana, Miyeko and de Lichtervelde, Lorenzo and Mul, Joram D and van de Peut, Diederik and Devos, Maxime and Simonis, Nicolas and Yildirim, Muhammed A and Cokol, Murat and Kao, Huey-Ling and De Smet, Anne-Sophie and Wang, Haidong and Schlaitz, Anne-Lore and Hao, Tong and Milstein, Stuart and Fan, Changyu and Tipsword, Mike and Drew, Kevin and Galli, Matilde and Rhrissorrakrai, Kahn and Drechsel, David and Koller, Daphne and Roth, Frederick P and Iakoucheva, Lilia M and Dunker, A Keith and Bonneau, Richard and Gunsalus, Kristin C and Hill, David E and Piano, Fabio and Tavernier, Jan and van den Heuvel, Sander and Hyman, Anthony A and Vidal, Marc},
  issn         = {0092-8674},
  journal      = {CELL},
  keyword      = {INTERACTION MAP,GENOME,2-HYBRID SCREENS,CENTROSOME MATURATION,SACCHAROMYCES-CEREVISIAE,NUCLEAR-PORE COMPLEX,CAENORHABDITIS-ELEGANS,IDENTIFICATION,RESOURCE,SCALE},
  language     = {eng},
  number       = {3},
  pages        = {534--545},
  title        = {A protein domain-based interactome network for C. elegans early embryogenesis},
  url          = {http://dx.doi.org/10.1016/j.cell.2008.07.009},
  volume       = {134},
  year         = {2008},
}

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