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Laser photofabrication of cell-containing hydrogel constructs

Aleksandr Ovsianikov, Severin Mühleder, Jan Torgersen, Zhiquan Li, Xiao-Hua Qin, Sandra Van Vlierberghe UGent, Peter Dubruel UGent, Wolfgang Holnthoner, Heinz Redl, Robert Liska, et al. (2014) LANGMUIR. 30(13). p.3787-3794
abstract
The two-photon polymerization (2PP) of photosensitive gelatin in the presence of living cells is reported. The 2PP technique is based on the localized cross-linking of photopolymers induced by femtosecond laser pulses. The availability of water-soluble photo-initiators (PI) suitable for 2PP is crucial for applying this method to cell-containing materials. Novel Pis developed by our group allow 2PP of formulations with up to 80% cell culture medium. The cytocompatibility of these Pis was evaluated by an MTT assay. The results of cell encapsulation by 2PP show the occurrence of cell damage within the laser-exposed regions. However, some cells located in the immediate vicinity and even within the 2PP-produced structures remain viable and can further proliferate. The control experiments demonstrate that the laser radiation itself does not damage the cells at the parameters used for 2PP. On the basis of these findings and the reports by other groups, we conclude that such localized cell damage is of a chemical origin and can be attributed to reactive species generated during 2PP. The viable cells trapped within the 2PP structures but not exposed to laser radiation continued to proliferate. The live/dead staining after 3 weeks revealed viable cells occupying most of the space available within the 3D hydrogel constructs. While some of the questions raised by this study remain open, the presented results indicate the general practicability of 2PP for 3D processing of cell-containing materials. The potential applications of this highly versatile approach span from precise engineering of 3D tissue models to the fabrication of cellular microarrays.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
2-PHOTON POLYMERIZATION, TISSUE ENGINEERING APPLICATIONS, IN-VITRO, 3D, MICROFABRICATION, SCAFFOLDS, PHOTOPOLYMERIZATION, CYTOCOMPATIBILITY, PHOTOINITIATORS, CYTOTOXICITY
journal title
LANGMUIR
Langmuir
volume
30
issue
13
pages
3787 - 3794
Web of Science type
Article
Web of Science id
000334572100017
JCR category
MATERIALS SCIENCE, MULTIDISCIPLINARY
JCR impact factor
4.457 (2014)
JCR rank
35/260 (2014)
JCR quartile
1 (2014)
ISSN
0743-7463
DOI
10.1021/la402346z
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4328800
handle
http://hdl.handle.net/1854/LU-4328800
date created
2014-03-11 13:38:38
date last changed
2016-12-19 15:43:06
@article{4328800,
  abstract     = {The two-photon polymerization (2PP) of photosensitive gelatin in the presence of living cells is reported. The 2PP technique is based on the localized cross-linking of photopolymers induced by femtosecond laser pulses. The availability of water-soluble photo-initiators (PI) suitable for 2PP is crucial for applying this method to cell-containing materials. Novel Pis developed by our group allow 2PP of formulations with up to 80\% cell culture medium. The cytocompatibility of these Pis was evaluated by an MTT assay. The results of cell encapsulation by 2PP show the occurrence of cell damage within the laser-exposed regions. However, some cells located in the immediate vicinity and even within the 2PP-produced structures remain viable and can further proliferate. The control experiments demonstrate that the laser radiation itself does not damage the cells at the parameters used for 2PP. On the basis of these findings and the reports by other groups, we conclude that such localized cell damage is of a chemical origin and can be attributed to reactive species generated during 2PP. The viable cells trapped within the 2PP structures but not exposed to laser radiation continued to proliferate. The live/dead staining after 3 weeks revealed viable cells occupying most of the space available within the 3D hydrogel constructs. While some of the questions raised by this study remain open, the presented results indicate the general practicability of 2PP for 3D processing of cell-containing materials. The potential applications of this highly versatile approach span from precise engineering of 3D tissue models to the fabrication of cellular microarrays.},
  author       = {Ovsianikov, Aleksandr and M{\"u}hleder, Severin and Torgersen, Jan and Li, Zhiquan and Qin, Xiao-Hua  and Van Vlierberghe, Sandra and Dubruel, Peter and Holnthoner, Wolfgang and Redl, Heinz and Liska, Robert and Stampfl, J{\"u}rgen},
  issn         = {0743-7463},
  journal      = {LANGMUIR},
  keyword      = {2-PHOTON POLYMERIZATION,TISSUE ENGINEERING APPLICATIONS,IN-VITRO,3D,MICROFABRICATION,SCAFFOLDS,PHOTOPOLYMERIZATION,CYTOCOMPATIBILITY,PHOTOINITIATORS,CYTOTOXICITY},
  language     = {eng},
  number       = {13},
  pages        = {3787--3794},
  title        = {Laser photofabrication of cell-containing hydrogel constructs},
  url          = {http://dx.doi.org/10.1021/la402346z},
  volume       = {30},
  year         = {2014},
}

Chicago
Ovsianikov, Aleksandr, Severin Mühleder, Jan Torgersen, Zhiquan Li, Xiao-Hua Qin, Sandra Van Vlierberghe, Peter Dubruel, et al. 2014. “Laser Photofabrication of Cell-containing Hydrogel Constructs.” Langmuir 30 (13): 3787–3794.
APA
Ovsianikov, A., Mühleder, S., Torgersen, J., Li, Z., Qin, X.-H., Van Vlierberghe, S., Dubruel, P., et al. (2014). Laser photofabrication of cell-containing hydrogel constructs. LANGMUIR, 30(13), 3787–3794.
Vancouver
1.
Ovsianikov A, Mühleder S, Torgersen J, Li Z, Qin X-H, Van Vlierberghe S, et al. Laser photofabrication of cell-containing hydrogel constructs. LANGMUIR. 2014;30(13):3787–94.
MLA
Ovsianikov, Aleksandr, Severin Mühleder, Jan Torgersen, et al. “Laser Photofabrication of Cell-containing Hydrogel Constructs.” LANGMUIR 30.13 (2014): 3787–3794. Print.