High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma
- Author
- An Vermeulen (UGent) , Marie Rosseel and Frans Belpaire
- Organization
- Abstract
- The enantiospecific determination of R- and S-hexobarbital in rat plasma is described. The method involves liquid-liquid extraction of racemic hexobarbital from plasma, separation of the underivatized enantiomers by high-performance liquid chromatography on an alpha-1-acid glycoprotein column and ultraviolet detection. The mobile phase consists of a phosphate buffer (pH 5.4) containing 0.4% 2-propanol as organic modifier. An alpha-1-acid glycoprotein guard column is used to increase the lifetime of the analytical column. Heptabarbital is the achiral internal standard. With detection limits of ca. 0.05-mu-g/ml for both R- and S-hexobarbital, the assay is suitable for pharmacokinetic studies of the enantiomers in rats.
- Keywords
- PHARMACOKINETICS, ENANTIOMERS, BLOOD, METABOLISM, SEPARATION
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 501.91 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-4325708
- MLA
- Vermeulen, An, Marie Rosseel, and Frans Belpaire. “High-performance Liquid Chromatographic Method for the Simultaneous Determination of R-(−)- and S-(+)-hexobarbital in Rat Plasma.” JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS 567.2 (1991): 472–479. Print.
- APA
- Vermeulen, An, Rosseel, M., & Belpaire, F. (1991). High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 567(2), 472–479.
- Chicago author-date
- Vermeulen, An, Marie Rosseel, and Frans Belpaire. 1991. “High-performance Liquid Chromatographic Method for the Simultaneous Determination of R-(−)- and S-(+)-hexobarbital in Rat Plasma.” Journal of Chromatography-biomedical Applications 567 (2): 472–479.
- Chicago author-date (all authors)
- Vermeulen, An, Marie Rosseel, and Frans Belpaire. 1991. “High-performance Liquid Chromatographic Method for the Simultaneous Determination of R-(−)- and S-(+)-hexobarbital in Rat Plasma.” Journal of Chromatography-biomedical Applications 567 (2): 472–479.
- Vancouver
- 1.Vermeulen A, Rosseel M, Belpaire F. High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS. 1991;567(2):472–9.
- IEEE
- [1]A. Vermeulen, M. Rosseel, and F. Belpaire, “High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma,” JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, vol. 567, no. 2, pp. 472–479, 1991.
@article{4325708,
abstract = {The enantiospecific determination of R- and S-hexobarbital in rat plasma is described. The method involves liquid-liquid extraction of racemic hexobarbital from plasma, separation of the underivatized enantiomers by high-performance liquid chromatography on an alpha-1-acid glycoprotein column and ultraviolet detection. The mobile phase consists of a phosphate buffer (pH 5.4) containing 0.4% 2-propanol as organic modifier. An alpha-1-acid glycoprotein guard column is used to increase the lifetime of the analytical column. Heptabarbital is the achiral internal standard. With detection limits of ca. 0.05-mu-g/ml for both R- and S-hexobarbital, the assay is suitable for pharmacokinetic studies of the enantiomers in rats.},
author = {Vermeulen, An and Rosseel, Marie and Belpaire, Frans},
issn = {0378-4347},
journal = {JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS},
keywords = {PHARMACOKINETICS,ENANTIOMERS,BLOOD,METABOLISM,SEPARATION},
language = {eng},
number = {2},
pages = {472--479},
title = {High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma},
url = {http://dx.doi.org/10.1016/0378-4347(91)80154-5},
volume = {567},
year = {1991},
}
- Altmetric
- View in Altmetric