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High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma

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Abstract
The enantiospecific determination of R- and S-hexobarbital in rat plasma is described. The method involves liquid-liquid extraction of racemic hexobarbital from plasma, separation of the underivatized enantiomers by high-performance liquid chromatography on an alpha-1-acid glycoprotein column and ultraviolet detection. The mobile phase consists of a phosphate buffer (pH 5.4) containing 0.4% 2-propanol as organic modifier. An alpha-1-acid glycoprotein guard column is used to increase the lifetime of the analytical column. Heptabarbital is the achiral internal standard. With detection limits of ca. 0.05-mu-g/ml for both R- and S-hexobarbital, the assay is suitable for pharmacokinetic studies of the enantiomers in rats.
Keywords
PHARMACOKINETICS, ENANTIOMERS, BLOOD, METABOLISM, SEPARATION

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MLA
Vermeulen, An, Marie Rosseel, and Frans Belpaire. “High-performance Liquid Chromatographic Method for the Simultaneous Determination of R-(−)- and S-(+)-hexobarbital in Rat Plasma.” JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS 567.2 (1991): 472–479. Print.
APA
Vermeulen, An, Rosseel, M., & Belpaire, F. (1991). High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 567(2), 472–479.
Chicago author-date
Vermeulen, An, Marie Rosseel, and Frans Belpaire. 1991. “High-performance Liquid Chromatographic Method for the Simultaneous Determination of R-(−)- and S-(+)-hexobarbital in Rat Plasma.” Journal of Chromatography-biomedical Applications 567 (2): 472–479.
Chicago author-date (all authors)
Vermeulen, An, Marie Rosseel, and Frans Belpaire. 1991. “High-performance Liquid Chromatographic Method for the Simultaneous Determination of R-(−)- and S-(+)-hexobarbital in Rat Plasma.” Journal of Chromatography-biomedical Applications 567 (2): 472–479.
Vancouver
1.
Vermeulen A, Rosseel M, Belpaire F. High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS. 1991;567(2):472–9.
IEEE
[1]
A. Vermeulen, M. Rosseel, and F. Belpaire, “High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma,” JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, vol. 567, no. 2, pp. 472–479, 1991.
@article{4325708,
  abstract     = {The enantiospecific determination of R- and S-hexobarbital in rat plasma is described. The method involves liquid-liquid extraction of racemic hexobarbital from plasma, separation of the underivatized enantiomers by high-performance liquid chromatography on an alpha-1-acid glycoprotein column and ultraviolet detection. The mobile phase consists of a phosphate buffer (pH 5.4) containing 0.4% 2-propanol as organic modifier. An alpha-1-acid glycoprotein guard column is used to increase the lifetime of the analytical column. Heptabarbital is the achiral internal standard. With detection limits of ca. 0.05-mu-g/ml for both R- and S-hexobarbital, the assay is suitable for pharmacokinetic studies of the enantiomers in rats.},
  author       = {Vermeulen, An and Rosseel, Marie and Belpaire, Frans},
  issn         = {0378-4347},
  journal      = {JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS},
  keywords     = {PHARMACOKINETICS,ENANTIOMERS,BLOOD,METABOLISM,SEPARATION},
  language     = {eng},
  number       = {2},
  pages        = {472--479},
  title        = {High-performance liquid chromatographic method for the simultaneous determination of R-(−)- and S-(+)-hexobarbital in rat plasma},
  url          = {http://dx.doi.org/10.1016/0378-4347(91)80154-5},
  volume       = {567},
  year         = {1991},
}

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