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Reversed-phase high-performance liquid chromatographic analysis of atenolol enantiomers in plasma after chiral derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate

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Abstract
A sensitive high-performance liquid chromatographic method for the determination of the enantiomers of atenolol in rat plasma has been developed. Racemic atenolol and practolol (internal standard) were extracted from alkalinized plasma (pH 12) into dichloromethane containing 3% (v/v) heptafluoro-1-butanol, and the organic layer was evaporated. The samples were derivatized with (+)-1-(9-fluorenyl)ethyl chloroformate at pH 8.5 for 30 min. After removal of excess reagent, the diastereomers were extracted into dichloromethane. The diastereomers were separated on a Microspher C18 column (3-mu-m) with a mobile phase of acetonitrile-sodium acetate buffer (0.01 M, pH 7) (50:50, v/v) at a flow-rate of 0.8 ml/min. Fluorescence detection (lambda-ex = 227 nm, lambda-em = 310 nm) was used. When 100-mu-l of plasma were used, the quantitation limit was 10 ng/ml for the atenolol enantiomers. The assay was applied to measure concentrations of atenolol enantiomers in plasma after intravenous administration of racemic atenolol to rats.
Keywords
URINE, SEPARATION, PHARMACOKINETICS

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MLA
Rosseel, Marie, An Vermeulen, and Frans Belpaire. “Reversed-phase High-performance Liquid Chromatographic Analysis of Atenolol Enantiomers in Plasma After Chiral Derivatization with (+)-1-(9-fluorenyl)ethyl Chloroformate.” JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS 568.1 (1991): 239–245. Print.
APA
Rosseel, M., Vermeulen, A., & Belpaire, F. (1991). Reversed-phase high-performance liquid chromatographic analysis of atenolol enantiomers in plasma after chiral derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 568(1), 239–245.
Chicago author-date
Rosseel, Marie, An Vermeulen, and Frans Belpaire. 1991. “Reversed-phase High-performance Liquid Chromatographic Analysis of Atenolol Enantiomers in Plasma After Chiral Derivatization with (+)-1-(9-fluorenyl)ethyl Chloroformate.” Journal of Chromatography-biomedical Applications 568 (1): 239–245.
Chicago author-date (all authors)
Rosseel, Marie, An Vermeulen, and Frans Belpaire. 1991. “Reversed-phase High-performance Liquid Chromatographic Analysis of Atenolol Enantiomers in Plasma After Chiral Derivatization with (+)-1-(9-fluorenyl)ethyl Chloroformate.” Journal of Chromatography-biomedical Applications 568 (1): 239–245.
Vancouver
1.
Rosseel M, Vermeulen A, Belpaire F. Reversed-phase high-performance liquid chromatographic analysis of atenolol enantiomers in plasma after chiral derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS. 1991;568(1):239–45.
IEEE
[1]
M. Rosseel, A. Vermeulen, and F. Belpaire, “Reversed-phase high-performance liquid chromatographic analysis of atenolol enantiomers in plasma after chiral derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate,” JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, vol. 568, no. 1, pp. 239–245, 1991.
@article{4325703,
  abstract     = {A sensitive high-performance liquid chromatographic method for the determination of the enantiomers of atenolol in rat plasma has been developed. Racemic atenolol and practolol (internal standard) were extracted from alkalinized plasma (pH 12) into dichloromethane containing 3% (v/v) heptafluoro-1-butanol, and the organic layer was evaporated. The samples were derivatized with (+)-1-(9-fluorenyl)ethyl chloroformate at pH 8.5 for 30 min. After removal of excess reagent, the diastereomers were extracted into dichloromethane. The diastereomers were separated on a Microspher C18 column (3-mu-m) with a mobile phase of acetonitrile-sodium acetate buffer (0.01 M, pH 7) (50:50, v/v) at a flow-rate of 0.8 ml/min. Fluorescence detection (lambda-ex = 227 nm, lambda-em = 310 nm) was used. When 100-mu-l of plasma were used, the quantitation limit was 10 ng/ml for the atenolol enantiomers. The assay was applied to measure concentrations of atenolol enantiomers in plasma after intravenous administration of racemic atenolol to rats.},
  author       = {Rosseel, Marie and Vermeulen, An and Belpaire, Frans},
  issn         = {0378-4347},
  journal      = {JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS},
  keywords     = {URINE,SEPARATION,PHARMACOKINETICS},
  language     = {eng},
  number       = {1},
  pages        = {239--245},
  title        = {Reversed-phase high-performance liquid chromatographic analysis of atenolol enantiomers in plasma after chiral derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate},
  url          = {http://dx.doi.org/10.1016/0378-4347(91)80359-K},
  volume       = {568},
  year         = {1991},
}

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