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Intensity modulated radiotherapy induces pro-inflammatory and pro-survival responses in prostate cancer patients

Houssein El Saghire, Charlot Vandevoorde, Piet Ost UGent, Pieter Monsieurs, Arlette Michaux, Gert De Meerleer UGent, Sarah Baatout UGent and Hubert Thierens UGent (2014) INTERNATIONAL JOURNAL OF ONCOLOGY. 44(4). p.1073-1083
abstract
Intensity modulated radiotherapy (IMRT) is one of the modern conformal radiotherapies that is widely used within the context of cancer patient treatment. It uses multiple radiation beams targeted to the tumor, however, large volumes of the body receive low doses of irradiation. Using γ-H2AX and global genome expression analysis, we studied the biological responses induced by low doses of ionizing radiation in prostate cancer patients following IMRT. By means of different bioinformatics analyses, we report that IMRT induced an inflammatory response via the induction of viral, adaptive, and innate immune signaling. In response to growth factors and immune-stimulatory signaling, positive regulation in the progression of cell cycle and DNA replication were induced. This denotes pro-inflammatory and pro-survival responses. Furthermore, double strand DNA breaks were induced in every patient 30 min after the treatment and remaining DNA repair and damage signaling continued after 18-24 h. Nine genes belonging to inflammatory responses (TLR3, SH2D1A and IL18), cell cycle progression (ORC4, SMC2 and CCDC99) and DNA damage and repair (RAD17, SMC6 and MRE11A) were confirmed by quantitative RT-PCR. This study emphasizes that the risk assessment of health effects from the out-of-field low doses during IMRT should be of concern, as these may increase the risk of secondary cancers and/or systemic inflammation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
radiotherapy, SET ENRICHMENT ANALYSIS, inflammatory response, low dose, ionizing radiation, microarray, IMRT, prostate cancer, γ-H2AX, TOLL-LIKE RECEPTOR-3, NF-KAPPA-B, IONIZING-RADIATION, GENE-EXPRESSION, 2ND MALIGNANCIES, WHOLE-BLOOD, T-CELLS, RISK, RNA
journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
Int. J. Oncol.
volume
44
issue
4
pages
1073 - 1083
Web of Science type
Article
Web of Science id
000334307100007
JCR category
ONCOLOGY
JCR impact factor
3.025 (2014)
JCR rank
96/211 (2014)
JCR quartile
2 (2014)
ISSN
1019-6439
DOI
10.3892/ijo.2014.2260
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
4324040
handle
http://hdl.handle.net/1854/LU-4324040
date created
2014-03-06 11:55:16
date last changed
2017-03-07 09:34:21
@article{4324040,
  abstract     = {Intensity modulated radiotherapy (IMRT) is one of the modern conformal radiotherapies that is widely used within the context of cancer patient treatment. It uses multiple radiation beams targeted to the tumor, however, large volumes of the body receive low doses of irradiation. Using \ensuremath{\gamma}-H2AX and global genome expression analysis, we studied the biological responses induced by low doses of ionizing radiation in prostate cancer patients following IMRT. By means of different bioinformatics analyses, we report that IMRT induced an inflammatory response via the induction of viral, adaptive, and innate immune signaling. In response to growth factors and immune-stimulatory signaling, positive regulation in the progression of cell cycle and DNA replication were induced. This denotes pro-inflammatory and pro-survival responses. Furthermore, double strand DNA breaks were induced in every patient 30 min after the treatment and remaining DNA repair and damage signaling continued after 18-24 h. Nine genes belonging to inflammatory responses (TLR3, SH2D1A and IL18), cell cycle progression (ORC4, SMC2 and CCDC99) and DNA damage and repair (RAD17, SMC6 and MRE11A) were confirmed by quantitative RT-PCR. This study emphasizes that the risk assessment of health effects from the out-of-field low doses during IMRT should be of concern, as these may increase the risk of secondary cancers and/or systemic inflammation.},
  author       = {El Saghire, Houssein and Vandevoorde, Charlot and Ost, Piet and Monsieurs, Pieter and Michaux, Arlette and De Meerleer, Gert and Baatout, Sarah and Thierens, Hubert},
  issn         = {1019-6439},
  journal      = {INTERNATIONAL JOURNAL OF ONCOLOGY},
  keyword      = {radiotherapy,SET ENRICHMENT ANALYSIS,inflammatory response,low dose,ionizing radiation,microarray,IMRT,prostate cancer,\ensuremath{\gamma}-H2AX,TOLL-LIKE RECEPTOR-3,NF-KAPPA-B,IONIZING-RADIATION,GENE-EXPRESSION,2ND MALIGNANCIES,WHOLE-BLOOD,T-CELLS,RISK,RNA},
  language     = {eng},
  number       = {4},
  pages        = {1073--1083},
  title        = {Intensity modulated radiotherapy induces pro-inflammatory and pro-survival responses in prostate cancer patients},
  url          = {http://dx.doi.org/10.3892/ijo.2014.2260},
  volume       = {44},
  year         = {2014},
}

Chicago
El Saghire, Houssein, Charlot Vandevoorde, Piet Ost, Pieter Monsieurs, Arlette Michaux, Gert De Meerleer, Sarah Baatout, and Hubert Thierens. 2014. “Intensity Modulated Radiotherapy Induces Pro-inflammatory and Pro-survival Responses in Prostate Cancer Patients.” International Journal of Oncology 44 (4): 1073–1083.
APA
El Saghire, H., Vandevoorde, C., Ost, P., Monsieurs, P., Michaux, A., De Meerleer, G., Baatout, S., et al. (2014). Intensity modulated radiotherapy induces pro-inflammatory and pro-survival responses in prostate cancer patients. INTERNATIONAL JOURNAL OF ONCOLOGY, 44(4), 1073–1083.
Vancouver
1.
El Saghire H, Vandevoorde C, Ost P, Monsieurs P, Michaux A, De Meerleer G, et al. Intensity modulated radiotherapy induces pro-inflammatory and pro-survival responses in prostate cancer patients. INTERNATIONAL JOURNAL OF ONCOLOGY. 2014;44(4):1073–83.
MLA
El Saghire, Houssein, Charlot Vandevoorde, Piet Ost, et al. “Intensity Modulated Radiotherapy Induces Pro-inflammatory and Pro-survival Responses in Prostate Cancer Patients.” INTERNATIONAL JOURNAL OF ONCOLOGY 44.4 (2014): 1073–1083. Print.