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Untargeted screening of secondary metabolites in fungal cultures and samples from mouldy indoor environments by time-of-flight mass spectrometry

Svetlana Malysheva UGent, Viviana Polizzi UGent, Antonio Moretti, Carlos Van Peteghem, Norbert De Kimpe UGent, Jan Van Bocxlaer UGent, José Diana Di Mavungu UGent and Sarah De Saeger UGent (2014) WORLD MYCOTOXIN JOURNAL. 7(1). p.35-44
abstract
Nowadays, complaints about poor indoor air quality have become common. The variety of indoor-air health problems include chronic fatigue, allergy, skin and eye irritation, and can be caused by several factors including fungi and their metabolites present in a building. The objective of this study was to establish a method for untargeted analysis of secondary fungal metabolites in indoor environments. As a detection technique, time-of-flight mass spectrometry was chosen, as it provided mass accuracy and higher sensitivity in full scan acquisition mode compared to tandem mass spectrometers. The method was first applied to fungal cultures, namely Penicillium brevicompactum and Chaetomium murorum, which were isolated from mouldy houses and grown on building materials in laboratory conditions for 7-21 days. Following the proposed strategy based on accurate mass measurement and post-acquisition data processing using Principal Component Analysis, roquefortine C, brevianamide A and mycophenolic acid were identified in Penicillium sp., while chaetoglobosin A was found to be produced by Chaetomium sp. Subsequently, samples from mouldy inhabited buildings were analyzed using the developed method. The actual presence of meleagrin was demonstrated in mouldy indoor environment. Applying the method to air and dust samples collected in these mouldy buildings, no metabolites were detected possibly due to generally low concentrations in these types of samples.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Sick Building Syndrome, indoor environments, time-of-flight, untargeted screening, WATER-DAMAGED BUILDINGS, PENICILLIUM-CHRYSOGENUM, CHAETOGLOBOSIN-A, MYCOTOXINS, METABOLOMICS, MAIZE, FOOD, QUANTIFICATION, PESTICIDES, DIERCKX
journal title
WORLD MYCOTOXIN JOURNAL
World Mycotoxin J.
volume
7
issue
1
pages
35 - 44
Web of Science type
Article
Web of Science id
000330838500003
JCR category
MYCOLOGY
JCR impact factor
2.157 (2014)
JCR rank
13/24 (2014)
JCR quartile
3 (2014)
ISSN
1875-0710
DOI
10.3920/WMJ2013.1595
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4323096
handle
http://hdl.handle.net/1854/LU-4323096
date created
2014-03-05 12:42:02
date last changed
2016-12-19 15:44:56
@article{4323096,
  abstract     = {Nowadays, complaints about poor indoor air quality have become common. The variety of indoor-air health problems include chronic fatigue, allergy, skin and eye irritation, and can be caused by several factors including fungi and their metabolites present in a building. The objective of this study was to establish a method for untargeted analysis of secondary fungal metabolites in indoor environments. As a detection technique, time-of-flight mass spectrometry was chosen, as it provided mass accuracy and higher sensitivity in full scan acquisition mode compared to tandem mass spectrometers. The method was first applied to fungal cultures, namely Penicillium brevicompactum and Chaetomium murorum, which were isolated from mouldy houses and grown on building materials in laboratory conditions for 7-21 days. Following the proposed strategy based on accurate mass measurement and post-acquisition data processing using Principal Component Analysis, roquefortine C, brevianamide A and mycophenolic acid were identified in Penicillium sp., while chaetoglobosin A was found to be produced by Chaetomium sp. Subsequently, samples from mouldy inhabited buildings were analyzed using the developed method. The actual presence of meleagrin was demonstrated in mouldy indoor environment. Applying the method to air and dust samples collected in these mouldy buildings, no metabolites were detected possibly due to generally low concentrations in these types of samples.},
  author       = {Malysheva, Svetlana and Polizzi, Viviana and Moretti, Antonio and Van Peteghem, Carlos and De Kimpe, Norbert and Van Bocxlaer, Jan and Diana Di Mavungu, Jos{\'e} and De Saeger, Sarah},
  issn         = {1875-0710},
  journal      = {WORLD MYCOTOXIN JOURNAL},
  keyword      = {Sick Building Syndrome,indoor environments,time-of-flight,untargeted screening,WATER-DAMAGED BUILDINGS,PENICILLIUM-CHRYSOGENUM,CHAETOGLOBOSIN-A,MYCOTOXINS,METABOLOMICS,MAIZE,FOOD,QUANTIFICATION,PESTICIDES,DIERCKX},
  language     = {eng},
  number       = {1},
  pages        = {35--44},
  title        = {Untargeted screening of secondary metabolites in fungal cultures and samples from mouldy indoor environments by time-of-flight mass spectrometry},
  url          = {http://dx.doi.org/10.3920/WMJ2013.1595},
  volume       = {7},
  year         = {2014},
}

Chicago
Malysheva, Svetlana, Viviana Polizzi, Antonio Moretti, Carlos Van Peteghem, Norbert De Kimpe, Jan Van Bocxlaer, José Diana Di Mavungu, and Sarah De Saeger. 2014. “Untargeted Screening of Secondary Metabolites in Fungal Cultures and Samples from Mouldy Indoor Environments by Time-of-flight Mass Spectrometry.” World Mycotoxin Journal 7 (1): 35–44.
APA
Malysheva, S., Polizzi, V., Moretti, A., Van Peteghem, C., De Kimpe, N., Van Bocxlaer, J., Diana Di Mavungu, J., et al. (2014). Untargeted screening of secondary metabolites in fungal cultures and samples from mouldy indoor environments by time-of-flight mass spectrometry. WORLD MYCOTOXIN JOURNAL, 7(1), 35–44.
Vancouver
1.
Malysheva S, Polizzi V, Moretti A, Van Peteghem C, De Kimpe N, Van Bocxlaer J, et al. Untargeted screening of secondary metabolites in fungal cultures and samples from mouldy indoor environments by time-of-flight mass spectrometry. WORLD MYCOTOXIN JOURNAL. 2014;7(1):35–44.
MLA
Malysheva, Svetlana, Viviana Polizzi, Antonio Moretti, et al. “Untargeted Screening of Secondary Metabolites in Fungal Cultures and Samples from Mouldy Indoor Environments by Time-of-flight Mass Spectrometry.” WORLD MYCOTOXIN JOURNAL 7.1 (2014): 35–44. Print.