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In vitro propagation

Hans Motte (UGent) , Stefaan Werbrouck (UGent) and Danny Geelen (UGent)
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Abstract
An important application of chemicals in plant biology is their use as plant growth regulators in in vitro plant tissue culture. In vitro propagation mainly relies on two classes of plant growth regulators: cytokinins and auxins. Together, they regulate cell proliferation and differentiation and are used to induce shoot or root organogenesis. Besides natural occurring cytokinins and auxins, many synthetic compounds are identified with similar action. These small molecules provide a diversified toolbox of plant growth regulators currently available for different applications in plant tissue culture. In this chapter, an overview of the different types of cytokinins and auxins is given, including their identification and mode of action. Special attention is given to new molecules identified via chemical screens. Finally, the application of the different types of cytokinins or auxins for specific processes required in plant tissue culture is discussed.
Keywords
Auxin, Cell proliferation, Tissue culture, Cell differentiation, in vitro propagation, Cytokinin

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Chicago
Motte, Hans, Stefaan Werbrouck, and Danny Geelen. 2014. “In Vitro Propagation.” In Plant Chemical Biology, ed. Dominique Audenaert and Paul Overvoorde, 263–287. Hoboken, NJ, USA: John Wiley & Sons.
APA
Motte, H., Werbrouck, S., & Geelen, D. (2014). In vitro propagation. In D. Audenaert & P. Overvoorde (Eds.), Plant chemical biology (pp. 263–287). Hoboken, NJ, USA: John Wiley & Sons.
Vancouver
1.
Motte H, Werbrouck S, Geelen D. In vitro propagation. In: Audenaert D, Overvoorde P, editors. Plant chemical biology. Hoboken, NJ, USA: John Wiley & Sons; 2014. p. 263–87.
MLA
Motte, Hans, Stefaan Werbrouck, and Danny Geelen. “In Vitro Propagation.” Plant Chemical Biology. Ed. Dominique Audenaert & Paul Overvoorde. Hoboken, NJ, USA: John Wiley & Sons, 2014. 263–287. Print.
@incollection{4317374,
  abstract     = {An important application of chemicals in plant biology is their use as plant growth regulators in in vitro plant tissue culture. In vitro propagation mainly relies on two classes of plant growth regulators: cytokinins and auxins. Together, they regulate cell proliferation and differentiation and are used to induce shoot or root organogenesis. Besides natural occurring cytokinins and auxins, many synthetic compounds are identified with similar action. These small molecules provide a diversified toolbox of plant growth regulators currently available for different applications in plant tissue culture. In this chapter, an overview of the different types of cytokinins and auxins is given, including their identification and mode of action. Special attention is given to new molecules identified via chemical screens. Finally, the application of the different types of cytokinins or auxins for specific processes required in plant tissue culture is discussed.},
  author       = {Motte, Hans and Werbrouck, Stefaan and Geelen, Danny},
  booktitle    = {Plant chemical biology},
  editor       = {Audenaert, Dominique and Overvoorde, Paul},
  isbn         = {9780470946695},
  keyword      = {Auxin,Cell proliferation,Tissue culture,Cell differentiation,in vitro propagation,Cytokinin},
  language     = {eng},
  pages        = {263--287},
  publisher    = {John Wiley \& Sons},
  title        = {In vitro propagation},
  year         = {2014},
}