Advanced search
1 file | 197.07 KB Add to list

Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy

(2008) ANALYTICAL CHEMISTRY. 80(6). p.2155-2160
Author
Organization
Abstract
This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.
Keywords
INFRARED-SPECTROSCOPY, SPECTROMETRY, CHROMATOGRAPHY, POLY-BETA-HYDROXYBUTYRATE, FLOW-CYTOMETRY, POLY(BETA-HYDROXYBUTYRIC ACID), ALCALIGENES-EUTROPHUS, PHB PRODUCTION, IDENTIFICATION, MICROORGANISMS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 197.07 KB

Citation

Please use this url to cite or link to this publication:

MLA
De Gelder, Joke, et al. “Monitoring Poly(3-Hydroxybutyrate) Production in Cupriavidus Necator DSM 428 (H16) with Raman Spectroscopy.” ANALYTICAL CHEMISTRY, vol. 80, no. 6, 2008, pp. 2155–60, doi:10.1021/ac702185d.
APA
De Gelder, J., Willemse-Erix, D., Scholtes, M. J., Sanchez, J. I., Maquelin, K., Vandenabeele, P., … De Vos, P. (2008). Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy. ANALYTICAL CHEMISTRY, 80(6), 2155–2160. https://doi.org/10.1021/ac702185d
Chicago author-date
De Gelder, Joke, Diana Willemse-Erix, Maarten J Scholtes, Jorge I Sanchez, Kees Maquelin, Peter Vandenabeele, Patrick De Boever, Gerwin J Puppels, Luc Moens, and Paul De Vos. 2008. “Monitoring Poly(3-Hydroxybutyrate) Production in Cupriavidus Necator DSM 428 (H16) with Raman Spectroscopy.” ANALYTICAL CHEMISTRY 80 (6): 2155–60. https://doi.org/10.1021/ac702185d.
Chicago author-date (all authors)
De Gelder, Joke, Diana Willemse-Erix, Maarten J Scholtes, Jorge I Sanchez, Kees Maquelin, Peter Vandenabeele, Patrick De Boever, Gerwin J Puppels, Luc Moens, and Paul De Vos. 2008. “Monitoring Poly(3-Hydroxybutyrate) Production in Cupriavidus Necator DSM 428 (H16) with Raman Spectroscopy.” ANALYTICAL CHEMISTRY 80 (6): 2155–2160. doi:10.1021/ac702185d.
Vancouver
1.
De Gelder J, Willemse-Erix D, Scholtes MJ, Sanchez JI, Maquelin K, Vandenabeele P, et al. Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy. ANALYTICAL CHEMISTRY. 2008;80(6):2155–60.
IEEE
[1]
J. De Gelder et al., “Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy,” ANALYTICAL CHEMISTRY, vol. 80, no. 6, pp. 2155–2160, 2008.
@article{431125,
  abstract     = {{This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.}},
  author       = {{De Gelder, Joke and Willemse-Erix, Diana and Scholtes, Maarten J and Sanchez, Jorge I and Maquelin, Kees and Vandenabeele, Peter and De Boever, Patrick and Puppels, Gerwin J and Moens, Luc and De Vos, Paul}},
  issn         = {{0003-2700}},
  journal      = {{ANALYTICAL CHEMISTRY}},
  keywords     = {{INFRARED-SPECTROSCOPY,SPECTROMETRY,CHROMATOGRAPHY,POLY-BETA-HYDROXYBUTYRATE,FLOW-CYTOMETRY,POLY(BETA-HYDROXYBUTYRIC ACID),ALCALIGENES-EUTROPHUS,PHB PRODUCTION,IDENTIFICATION,MICROORGANISMS}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{2155--2160}},
  title        = {{Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy}},
  url          = {{http://doi.org/10.1021/ac702185d}},
  volume       = {{80}},
  year         = {{2008}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: