Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy
- Author
- Joke De Gelder (UGent) , Diana Willemse-Erix, Maarten J Scholtes, Jorge I Sanchez, Kees Maquelin, Peter Vandenabeele (UGent) , Patrick De Boever, Gerwin J Puppels, Luc Moens (UGent) and Paul De Vos (UGent)
- Organization
- Abstract
- This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.
- Keywords
- INFRARED-SPECTROSCOPY, SPECTROMETRY, CHROMATOGRAPHY, POLY-BETA-HYDROXYBUTYRATE, FLOW-CYTOMETRY, POLY(BETA-HYDROXYBUTYRIC ACID), ALCALIGENES-EUTROPHUS, PHB PRODUCTION, IDENTIFICATION, MICROORGANISMS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-431125
- MLA
- De Gelder, Joke, et al. “Monitoring Poly(3-Hydroxybutyrate) Production in Cupriavidus Necator DSM 428 (H16) with Raman Spectroscopy.” ANALYTICAL CHEMISTRY, vol. 80, no. 6, 2008, pp. 2155–60, doi:10.1021/ac702185d.
- APA
- De Gelder, J., Willemse-Erix, D., Scholtes, M. J., Sanchez, J. I., Maquelin, K., Vandenabeele, P., … De Vos, P. (2008). Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy. ANALYTICAL CHEMISTRY, 80(6), 2155–2160. https://doi.org/10.1021/ac702185d
- Chicago author-date
- De Gelder, Joke, Diana Willemse-Erix, Maarten J Scholtes, Jorge I Sanchez, Kees Maquelin, Peter Vandenabeele, Patrick De Boever, Gerwin J Puppels, Luc Moens, and Paul De Vos. 2008. “Monitoring Poly(3-Hydroxybutyrate) Production in Cupriavidus Necator DSM 428 (H16) with Raman Spectroscopy.” ANALYTICAL CHEMISTRY 80 (6): 2155–60. https://doi.org/10.1021/ac702185d.
- Chicago author-date (all authors)
- De Gelder, Joke, Diana Willemse-Erix, Maarten J Scholtes, Jorge I Sanchez, Kees Maquelin, Peter Vandenabeele, Patrick De Boever, Gerwin J Puppels, Luc Moens, and Paul De Vos. 2008. “Monitoring Poly(3-Hydroxybutyrate) Production in Cupriavidus Necator DSM 428 (H16) with Raman Spectroscopy.” ANALYTICAL CHEMISTRY 80 (6): 2155–2160. doi:10.1021/ac702185d.
- Vancouver
- 1.De Gelder J, Willemse-Erix D, Scholtes MJ, Sanchez JI, Maquelin K, Vandenabeele P, et al. Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy. ANALYTICAL CHEMISTRY. 2008;80(6):2155–60.
- IEEE
- [1]J. De Gelder et al., “Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy,” ANALYTICAL CHEMISTRY, vol. 80, no. 6, pp. 2155–2160, 2008.
@article{431125, abstract = {{This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L-1 culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.}}, author = {{De Gelder, Joke and Willemse-Erix, Diana and Scholtes, Maarten J and Sanchez, Jorge I and Maquelin, Kees and Vandenabeele, Peter and De Boever, Patrick and Puppels, Gerwin J and Moens, Luc and De Vos, Paul}}, issn = {{0003-2700}}, journal = {{ANALYTICAL CHEMISTRY}}, keywords = {{INFRARED-SPECTROSCOPY,SPECTROMETRY,CHROMATOGRAPHY,POLY-BETA-HYDROXYBUTYRATE,FLOW-CYTOMETRY,POLY(BETA-HYDROXYBUTYRIC ACID),ALCALIGENES-EUTROPHUS,PHB PRODUCTION,IDENTIFICATION,MICROORGANISMS}}, language = {{eng}}, number = {{6}}, pages = {{2155--2160}}, title = {{Monitoring poly(3-hydroxybutyrate) production in Cupriavidus necator DSM 428 (H16) with Raman spectroscopy}}, url = {{http://doi.org/10.1021/ac702185d}}, volume = {{80}}, year = {{2008}}, }
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