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Contribution of the Ly49E natural killer receptor in the immune response to Plasmodium berghei infection and control of hepatic parasite development

Jessica Filtjens (UGent) , Lander Foquet (UGent) , Sylvie Taveirne (UGent) , Els Van Ammel (UGent) , Mandy Vanhees (UGent) , Aline Van Acker (UGent) , Tessa Kerre (UGent) , Tom Taghon (UGent) , Bart Vandekerckhove (UGent) , Jean Plum (UGent) , et al.
(2014) PLOS ONE. 9(1).
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Abstract
Natural killer (NK) cells have different roles in the host response against Plasmodium-induced malaria depending on the stage of infection. Liver NK cells have a protective role during the initial hepatic stage of infection by production of the TH1-type cytokines IFN-γ and TNF-α. In the subsequent erythrocytic stage of infection, NK cells also induce protection through Th1-type cytokines but, in addition, may also promote development of cerebral malaria via CXCR3-induction on CD8+ T cells resulting in migration of these cells to the brain. We have recently shown that the regulatory Ly49E NK receptor is expressed on liver NK cells in particular. The main objective of this study was therefore to examine the role of Ly49E expression in the immune response upon Plasmodium berghei ANKA infection, for which we compared wild type (WT) to Ly49E knockout (KO) mice. We show that the parasitemia was higher at the early stage, i.e. at days 6–7 of Plasmodium berghei ANKA infection in Ly49E KO mice, which correlated with lower induction of CD69, IFN-γ and TNF-α in DX5− liver NK cells at day 5 post-infection. At later stages, these differences faded. There was also no difference in the kinetics and the percentage of cerebral malaria development and in lymphocyte CXCR3 expression in WT versus Ly49E KO mice. Collectively, we show that the immune response against Plasmodium berghei ANKA infection is not drastically affected in Ly49E KO mice. Although NK cells play a crucial role in Plasmodium infection and Ly49E is highly expressed on liver NK cells, the Ly49E NK receptor only has a temporarily role in the immune control of this parasite.
Keywords
NK CELL RECOGNITION, MURINE CEREBRAL MALARIA, T-CELLS, MISSING SELF, PROTECTIVE IMMUNITY, INTERFERON-GAMMA, LIVER-STAGE, PATHOGENESIS, EXPRESSION, MHC

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MLA
Filtjens, Jessica, Lander Foquet, Sylvie Taveirne, et al. “Contribution of the Ly49E Natural Killer Receptor in the Immune Response to Plasmodium Berghei Infection and Control of Hepatic Parasite Development.” PLOS ONE 9.1 (2014): n. pag. Print.
APA
Filtjens, J., Foquet, L., Taveirne, S., Van Ammel, E., Vanhees, M., Van Acker, A., Kerre, T., et al. (2014). Contribution of the Ly49E natural killer receptor in the immune response to Plasmodium berghei infection and control of hepatic parasite development. PLOS ONE, 9(1).
Chicago author-date
Filtjens, Jessica, Lander Foquet, Sylvie Taveirne, Els Van Ammel, Mandy Vanhees, Aline Van Acker, Tessa Kerre, et al. 2014. “Contribution of the Ly49E Natural Killer Receptor in the Immune Response to Plasmodium Berghei Infection and Control of Hepatic Parasite Development.” Plos One 9 (1).
Chicago author-date (all authors)
Filtjens, Jessica, Lander Foquet, Sylvie Taveirne, Els Van Ammel, Mandy Vanhees, Aline Van Acker, Tessa Kerre, Tom Taghon, Bart Vandekerckhove, Jean Plum, Philippe Van den Steen, and Georges Leclercq. 2014. “Contribution of the Ly49E Natural Killer Receptor in the Immune Response to Plasmodium Berghei Infection and Control of Hepatic Parasite Development.” Plos One 9 (1).
Vancouver
1.
Filtjens J, Foquet L, Taveirne S, Van Ammel E, Vanhees M, Van Acker A, et al. Contribution of the Ly49E natural killer receptor in the immune response to Plasmodium berghei infection and control of hepatic parasite development. PLOS ONE. 2014;9(1).
IEEE
[1]
J. Filtjens et al., “Contribution of the Ly49E natural killer receptor in the immune response to Plasmodium berghei infection and control of hepatic parasite development,” PLOS ONE, vol. 9, no. 1, 2014.
@article{4300721,
  abstract     = {{Natural killer (NK) cells have different roles in the host response against Plasmodium-induced malaria depending on the stage of infection. Liver NK cells have a protective role during the initial hepatic stage of infection by production of the TH1-type cytokines IFN-γ and TNF-α. In the subsequent erythrocytic stage of infection, NK cells also induce protection through Th1-type cytokines but, in addition, may also promote development of cerebral malaria via CXCR3-induction on CD8+ T cells resulting in migration of these cells to the brain. We have recently shown that the regulatory Ly49E NK receptor is expressed on liver NK cells in particular. The main objective of this study was therefore to examine the role of Ly49E expression in the immune response upon Plasmodium berghei ANKA infection, for which we compared wild type (WT) to Ly49E knockout (KO) mice. We show that the parasitemia was higher at the early stage, i.e. at days 6–7 of Plasmodium berghei ANKA infection in Ly49E KO mice, which correlated with lower induction of CD69, IFN-γ and TNF-α in DX5− liver NK cells at day 5 post-infection. At later stages, these differences faded. There was also no difference in the kinetics and the percentage of cerebral malaria development and in lymphocyte CXCR3 expression in WT versus Ly49E KO mice. Collectively, we show that the immune response against Plasmodium berghei ANKA infection is not drastically affected in Ly49E KO mice. Although NK cells play a crucial role in Plasmodium infection and Ly49E is highly expressed on liver NK cells, the Ly49E NK receptor only has a temporarily role in the immune control of this parasite.}},
  articleno    = {{e87463}},
  author       = {{Filtjens, Jessica and Foquet, Lander and Taveirne, Sylvie and Van Ammel, Els and Vanhees, Mandy and Van Acker, Aline and Kerre, Tessa and Taghon, Tom and Vandekerckhove, Bart and Plum, Jean and Van den Steen, Philippe and Leclercq, Georges}},
  issn         = {{1932-6203}},
  journal      = {{PLOS ONE}},
  keywords     = {{NK CELL RECOGNITION,MURINE CEREBRAL MALARIA,T-CELLS,MISSING SELF,PROTECTIVE IMMUNITY,INTERFERON-GAMMA,LIVER-STAGE,PATHOGENESIS,EXPRESSION,MHC}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{9}},
  title        = {{Contribution of the Ly49E natural killer receptor in the immune response to Plasmodium berghei infection and control of hepatic parasite development}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0087463}},
  volume       = {{9}},
  year         = {{2014}},
}

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