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Predicting drug penetration across the blood-brain barrier: comparison of different stationary phases for immobilized artificial membrane liquid chromatography

Mike De Vrieze (UGent) , Frederic Lynen (UGent) , Dieter Verzele, Roman Szucs (UGent) and Patrick Sandra (UGent)
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Abstract
Several in vitro methods have been tested for their ability to predict drug penetration across the blood-brain barrier into the central nervous system. In this article, the performance of three stationary phases for immobilized artificial membrane (IAM) liquid chromatographic approaches were compared on a set of 49 compounds. IAM liquid chromatography measurements were performed with Dulbecco’s phosphate-buffered saline and methanol as organic modifier in the mobile phase. Transport across the blood-brain barrier (log BB) was predicted using computed descriptor data and the retention factor of all compounds. All data were correlated with experimental log BB values and the relative performance of the approaches was studied. The IAM.PC.DD2 column proved to be the best suited for prediction of log BB values, although all three columns performed very good.
Keywords
Blood-brain barrier, Immobilized Artificial Membranes

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Chicago
De Vrieze, Mike, Frederic Lynen, Dieter Verzele, Roman Szucs, and Patrick Sandra. 2014. “Predicting Drug Penetration Across the Blood-brain Barrier: Comparison of Different Stationary Phases for Immobilized Artificial Membrane Liquid Chromatography.” In 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts.
APA
De Vrieze, M., Lynen, F., Verzele, D., Szucs, R., & Sandra, P. (2014). Predicting drug penetration across the blood-brain barrier: comparison of different stationary phases for immobilized artificial membrane liquid chromatography. 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts. Presented at the 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) ; 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3).
Vancouver
1.
De Vrieze M, Lynen F, Verzele D, Szucs R, Sandra P. Predicting drug penetration across the blood-brain barrier: comparison of different stationary phases for immobilized artificial membrane liquid chromatography. 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts. 2014.
MLA
De Vrieze, Mike, Frederic Lynen, Dieter Verzele, et al. “Predicting Drug Penetration Across the Blood-brain Barrier: Comparison of Different Stationary Phases for Immobilized Artificial Membrane Liquid Chromatography.” 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts. 2014. Print.
@inproceedings{4295548,
  abstract     = {Several in vitro methods have been tested for their ability to predict drug penetration across the blood-brain barrier into the central nervous system. In this article, the performance of three stationary phases for immobilized artificial membrane (IAM) liquid chromatographic approaches were compared on a set of 49 compounds. IAM liquid chromatography measurements were performed with Dulbecco{\textquoteright}s phosphate-buffered saline and methanol as organic modifier in the mobile phase. Transport across the blood-brain barrier (log BB) was predicted using computed descriptor data and the retention factor of all compounds. All data were correlated with experimental log BB values and the relative performance of the approaches was studied. The IAM.PC.DD2 column proved to be the best suited for prediction of log BB values, although all three columns performed very good.},
  author       = {De Vrieze, Mike and Lynen, Frederic and Verzele, Dieter and Szucs, Roman and Sandra, Patrick},
  booktitle    = {13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts},
  keyword      = {Blood-brain barrier,Immobilized Artificial Membranes},
  language     = {eng},
  location     = {Brugge, Belgium},
  title        = {Predicting drug penetration across the blood-brain barrier: comparison of different stationary phases for immobilized artificial membrane liquid chromatography},
  year         = {2014},
}