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Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase

Rebecca Van Acker (UGent) , Jean-Charles Leplé, Dirk Aerts (UGent) , Veronique Storme (UGent) , Geert Goeminne (UGent) , Bart Ivens (UGent) , Frédéric Légée, Catherine Lapierre, Kathleen Piens, Marc Van Montagu (UGent) , et al.
(2014) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 111(2). p.845-850
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Abstract
Lignin is one of the main factors determining recalcitrance to enzymatic processing of lignocellulosic biomass. Poplars (Populus tremula x Populus alba) down-regulated for cinnamoyl-CoA reductase (CCR), the enzyme catalyzing the first step in the monolignol-specific branch of the lignin biosynthetic pathway, were grown in field trials in Belgium and France under short-rotation coppice culture. Wood samples were classified according to the intensity of the red xylem coloration typically associated with CCR down-regulation. Saccharification assays under different pretreatment conditions (none, two alkaline, and one acid pretreatment) and simultaneous saccharification and fermentation assays showed that wood from the most affected transgenic trees had up to 161% increased ethanol yield. Fermentations of combined material from the complete set of 20-mo-old CCR-down-regulated trees, including bark and less efficiently down-regulated trees, still yielded similar to 20% more ethanol on a weight basis. However, strong down-regulation of CCR also affected biomass yield. We conclude that CCR down-regulation may become a successful strategy to improve biomass processing if the variability in down-regulation and the yield penalty can be overcome.
Keywords
LIGNIFICATION, PLANTS, ARABIDOPSIS-THALIANA, DOWN-REGULATION, second-generation bioenergy, GM, bioethanol, BIOFUELS, BIOMASS, TREES, METABOLISM, EXPRESSION, LIGNIN BIOSYNTHESIS PERTURBATIONS

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Citation

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MLA
Van Acker, Rebecca, et al. “Improved Saccharification and Ethanol Yield from Field-Grown Transgenic Poplar Deficient in Cinnamoyl-CoA Reductase.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 111, no. 2, 2014, pp. 845–50, doi:10.1073/pnas.321673111.
APA
Van Acker, R., Leplé, J.-C., Aerts, D., Storme, V., Goeminne, G., Ivens, B., … Boerjan, W. (2014). Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(2), 845–850. https://doi.org/10.1073/pnas.321673111
Chicago author-date
Van Acker, Rebecca, Jean-Charles Leplé, Dirk Aerts, Veronique Storme, Geert Goeminne, Bart Ivens, Frédéric Légée, et al. 2014. “Improved Saccharification and Ethanol Yield from Field-Grown Transgenic Poplar Deficient in Cinnamoyl-CoA Reductase.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111 (2): 845–50. https://doi.org/10.1073/pnas.321673111.
Chicago author-date (all authors)
Van Acker, Rebecca, Jean-Charles Leplé, Dirk Aerts, Veronique Storme, Geert Goeminne, Bart Ivens, Frédéric Légée, Catherine Lapierre, Kathleen Piens, Marc Van Montagu, Nicholas Santoro, Clifton E Foster, John Ralph, Wim Soetaert, Gilles Pilate, and Wout Boerjan. 2014. “Improved Saccharification and Ethanol Yield from Field-Grown Transgenic Poplar Deficient in Cinnamoyl-CoA Reductase.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111 (2): 845–850. doi:10.1073/pnas.321673111.
Vancouver
1.
Van Acker R, Leplé J-C, Aerts D, Storme V, Goeminne G, Ivens B, et al. Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2014;111(2):845–50.
IEEE
[1]
R. Van Acker et al., “Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 111, no. 2, pp. 845–850, 2014.
@article{4294836,
  abstract     = {{Lignin is one of the main factors determining recalcitrance to enzymatic processing of lignocellulosic biomass. Poplars (Populus tremula x Populus alba) down-regulated for cinnamoyl-CoA reductase (CCR), the enzyme catalyzing the first step in the monolignol-specific branch of the lignin biosynthetic pathway, were grown in field trials in Belgium and France under short-rotation coppice culture. Wood samples were classified according to the intensity of the red xylem coloration typically associated with CCR down-regulation. Saccharification assays under different pretreatment conditions (none, two alkaline, and one acid pretreatment) and simultaneous saccharification and fermentation assays showed that wood from the most affected transgenic trees had up to 161% increased ethanol yield. Fermentations of combined material from the complete set of 20-mo-old CCR-down-regulated trees, including bark and less efficiently down-regulated trees, still yielded similar to 20% more ethanol on a weight basis. However, strong down-regulation of CCR also affected biomass yield. We conclude that CCR down-regulation may become a successful strategy to improve biomass processing if the variability in down-regulation and the yield penalty can be overcome.}},
  author       = {{Van Acker, Rebecca and Leplé, Jean-Charles and Aerts, Dirk and Storme, Veronique and Goeminne, Geert and Ivens, Bart and Légée, Frédéric and Lapierre, Catherine and Piens, Kathleen and Van Montagu, Marc and Santoro, Nicholas and Foster, Clifton E and Ralph, John and Soetaert, Wim and Pilate, Gilles and Boerjan, Wout}},
  issn         = {{0027-8424}},
  journal      = {{PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}},
  keywords     = {{LIGNIFICATION,PLANTS,ARABIDOPSIS-THALIANA,DOWN-REGULATION,second-generation bioenergy,GM,bioethanol,BIOFUELS,BIOMASS,TREES,METABOLISM,EXPRESSION,LIGNIN BIOSYNTHESIS PERTURBATIONS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{845--850}},
  title        = {{Improved saccharification and ethanol yield from field-grown transgenic poplar deficient in cinnamoyl-CoA reductase}},
  url          = {{http://doi.org/10.1073/pnas.321673111}},
  volume       = {{111}},
  year         = {{2014}},
}
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