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A mildly inducible and selective cross-link methodology for RNA duplexes

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Abstract
We here report on the furan oxidation methodology for interstrand cross-linking of RNA duplexes, which have a different structure and are more stiff, reactive and labile than their DNA counterparts. Through this mildly inducible approach, natural unmodified RNA can be selectively cross-linked in high yield. The method therefore has direct applications in the increasing number of RNA based technologies.
Keywords
CLICK LIGATION, NONCODING RNAS, DNA, FURAN, OLIGONUCLEOTIDE, ALKYLATION, STABILITY, OXIDATION, METHYL, AZIDES

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Citation

Please use this url to cite or link to this publication:

Chicago
Carrette, Lieselot, Ellen Gyssels, Joke Loncke, and Annemieke Madder. 2014. “A Mildly Inducible and Selective Cross-link Methodology for RNA Duplexes.” Organic & Biomolecular Chemistry 12 (6): 931–935.
APA
Carrette, L., Gyssels, E., Loncke, J., & Madder, A. (2014). A mildly inducible and selective cross-link methodology for RNA duplexes. ORGANIC & BIOMOLECULAR CHEMISTRY, 12(6), 931–935.
Vancouver
1.
Carrette L, Gyssels E, Loncke J, Madder A. A mildly inducible and selective cross-link methodology for RNA duplexes. ORGANIC & BIOMOLECULAR CHEMISTRY. 2014;12(6):931–5.
MLA
Carrette, Lieselot, Ellen Gyssels, Joke Loncke, et al. “A Mildly Inducible and Selective Cross-link Methodology for RNA Duplexes.” ORGANIC & BIOMOLECULAR CHEMISTRY 12.6 (2014): 931–935. Print.
@article{4293631,
  abstract     = {We here report on the furan oxidation methodology for interstrand cross-linking of RNA duplexes, which have a different structure and are more stiff, reactive and labile than their DNA counterparts. Through this mildly inducible approach, natural unmodified RNA can be selectively cross-linked in high yield. The method therefore has direct applications in the increasing number of RNA based technologies.},
  author       = {Carrette, Lieselot and Gyssels, Ellen and Loncke, Joke and Madder, Annemieke},
  issn         = {1477-0520},
  journal      = {ORGANIC \& BIOMOLECULAR CHEMISTRY},
  language     = {eng},
  number       = {6},
  pages        = {931--935},
  title        = {A mildly inducible and selective cross-link methodology for RNA duplexes},
  url          = {http://dx.doi.org/10.1039/C3OB42374C},
  volume       = {12},
  year         = {2014},
}

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