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Toxicity inspired cross-linking for probing DNA–peptide interactions

(2013) BIOCONJUGATE CHEMISTRY. 24(12). p.2008-2014
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Abstract
A cross-linking methodology for the study of DNA–protein interactions is described. The method is inspired by the metabolic activation of furans causing toxic DNA damage, including DNA–protein cross-links (DPC). The furan moiety, representing a latent functionality, is easily incorporated into oligonucleotides, and can be activated on demand to release a reactive aldehyde. Reaction with nucleophilic lysine side chains is shown to be distance-sensitive and allows for site-selective DPC formation.
Keywords
REACTIVE DIALDEHYDE GROUPS, ABASIC SITE, FURAN, PROTEIN-INTERACTIONS, MUTAGENICITY, TRANSCRIPTION, OLIGONUCLEOTIDES, COMPLEXES, RNA, METABOLISM

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Citation

Please use this url to cite or link to this publication:

Chicago
Carrette, Lieselot, Takashi Morii, and Annemieke Madder. 2013. “Toxicity Inspired Cross-linking for Probing DNA–peptide Interactions.” Bioconjugate Chemistry 24 (12): 2008–2014.
APA
Carrette, L., Morii, T., & Madder, A. (2013). Toxicity inspired cross-linking for probing DNA–peptide interactions. BIOCONJUGATE CHEMISTRY, 24(12), 2008–2014.
Vancouver
1.
Carrette L, Morii T, Madder A. Toxicity inspired cross-linking for probing DNA–peptide interactions. BIOCONJUGATE CHEMISTRY. 2013;24(12):2008–14.
MLA
Carrette, Lieselot, Takashi Morii, and Annemieke Madder. “Toxicity Inspired Cross-linking for Probing DNA–peptide Interactions.” BIOCONJUGATE CHEMISTRY 24.12 (2013): 2008–2014. Print.
@article{4293539,
  abstract     = {A cross-linking methodology for the study of DNA--protein interactions is described. The method is inspired by the metabolic activation of furans causing toxic DNA damage, including DNA--protein cross-links (DPC). The furan moiety, representing a latent functionality, is easily incorporated into oligonucleotides, and can be activated on demand to release a reactive aldehyde. Reaction with nucleophilic lysine side chains is shown to be distance-sensitive and allows for site-selective DPC formation.},
  author       = {Carrette, Lieselot and Morii, Takashi and Madder, Annemieke},
  issn         = {1043-1802},
  journal      = {BIOCONJUGATE CHEMISTRY},
  language     = {eng},
  number       = {12},
  pages        = {2008--2014},
  title        = {Toxicity inspired cross-linking for probing DNA--peptide interactions},
  url          = {http://dx.doi.org/10.1021/bc400327q},
  volume       = {24},
  year         = {2013},
}

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