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State-of-the-art non-targeted metabolomics in the study of chronic kidney disease

(2014) METABOLOMICS. 10(3). p.425-442
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Abstract
Here we report a metabolomics discovery study conducted on blood serum samples of patients in different stages of chronic kidney disease (CKD). Metabolites were monitored on a quality controlled holistic platform combining reversed-phase liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry in both negative and positive ionization mode and gas chromatography coupled to quadrupole mass spectrometry. A substantial portion of the serum metabolome was thereby covered. Eighty-five metabolites were shown to evolve with CKD progression of which 43 metabolites were a confirmation of earlier reported uremic retention solutes and/or uremic toxins. Thirty-one unique metabolites were revealed which were increasing significantly throughout CKD progression, by a factor surpassing the level observed for creatinine, the currently used biomarker for kidney function. Additionally, 11 unique metabolites showed a decreasing trend.
Keywords
Metabolomics, LC–MS, GC–MS, Chronic kidney disease, Q-TOF, Serum, TANDEM MASS-SPECTROMETRY, CHRONIC-RENAL-FAILURE, UREMIC TOXINS, HEMODIALYSIS-PATIENTS, SERUM METABONOMICS, METLIN DATABASE, HYALURONIC-ACID, IDENTIFICATION, PERFORMANCE, BIOMARKERS

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Citation

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Chicago
Boelaert, Jente, Ruben t’ Kindt, Eva Schepers, Lucie Jorge, Griet Glorieux, Nathalie Neirynck, Frederic Lynen, Patrick Sandra, Raymond Vanholder, and Koen Sandra. 2014. “State-of-the-art Non-targeted Metabolomics in the Study of Chronic Kidney Disease.” Metabolomics 10 (3): 425–442.
APA
Boelaert, Jente, t’ Kindt, R., Schepers, E., Jorge, L., Glorieux, G., Neirynck, N., Lynen, F., et al. (2014). State-of-the-art non-targeted metabolomics in the study of chronic kidney disease. METABOLOMICS, 10(3), 425–442.
Vancouver
1.
Boelaert J, t’ Kindt R, Schepers E, Jorge L, Glorieux G, Neirynck N, et al. State-of-the-art non-targeted metabolomics in the study of chronic kidney disease. METABOLOMICS. 2014;10(3):425–42.
MLA
Boelaert, Jente, Ruben t’ Kindt, Eva Schepers, et al. “State-of-the-art Non-targeted Metabolomics in the Study of Chronic Kidney Disease.” METABOLOMICS 10.3 (2014): 425–442. Print.
@article{4292283,
  abstract     = {Here we report a metabolomics discovery study conducted on blood serum samples of patients in different stages of chronic kidney disease (CKD). Metabolites were monitored on a quality controlled holistic platform combining reversed-phase liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry in both negative and positive ionization mode and gas chromatography coupled to quadrupole mass spectrometry. A substantial portion of the serum metabolome was thereby covered. Eighty-five metabolites were shown to evolve with CKD progression of which 43 metabolites were a confirmation of earlier reported uremic retention solutes and/or uremic toxins. Thirty-one unique metabolites were revealed which were increasing significantly throughout CKD progression, by a factor surpassing the level observed for creatinine, the currently used biomarker for kidney function. Additionally, 11 unique metabolites showed a decreasing trend.},
  author       = {Boelaert, Jente and t'Kindt, Ruben and Schepers, Eva and Jorge, Lucie and Glorieux, Griet and Neirynck, Nathalie and Lynen, Frederic and Sandra, Patrick and Vanholder, Raymond and Sandra, Koen},
  issn         = {1573-3882},
  journal      = {METABOLOMICS},
  keyword      = {Metabolomics,LC--MS,GC--MS,Chronic kidney disease,Q-TOF,Serum,TANDEM MASS-SPECTROMETRY,CHRONIC-RENAL-FAILURE,UREMIC TOXINS,HEMODIALYSIS-PATIENTS,SERUM METABONOMICS,METLIN DATABASE,HYALURONIC-ACID,IDENTIFICATION,PERFORMANCE,BIOMARKERS},
  language     = {eng},
  number       = {3},
  pages        = {425--442},
  title        = {State-of-the-art non-targeted metabolomics in the study of chronic kidney disease},
  url          = {http://dx.doi.org/10.1007/s11306-013-0592-z},
  volume       = {10},
  year         = {2014},
}

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