A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study
- Author
- Rudy GE Van Eijsden, Catherine Stassen, Luk Daenen, Sebastiaan E Van Mulders, Prashant M Bapat, Verena Siewers, Katty VY Goossens, Jens Nielsen, Freddy R Delvaux, Paul Van Hummelen, Bart Devreese (UGent) and Ronnie G Willaert
- Organization
- Abstract
- Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.
- Keywords
- FROZEN, NUMBER, YEAST, PCR, DNA, METABOLISM, PRESERVATION, MESSENGER-RNA, TISSUE STORAGE METHODS, Transcriptomics, Saccharomyces cerevisiae, RNAlater, Omics, Fluxomics, Genomics
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-4292013
- Chicago
- Van Eijsden, Rudy GE, Catherine Stassen, Luk Daenen, Sebastiaan E Van Mulders, Prashant M Bapat, Verena Siewers, Katty VY Goossens, et al. 2013. “A Universal Fixation Method Based on Quaternary Ammonium Salts (RNAlater) for Omics-technologies: Saccharomyces Cerevisiae as a Case Study.” Biotechnology Letters 35 (6): 891–900.
- APA
- Van Eijsden, R. G., Stassen, C., Daenen, L., Van Mulders, S. E., Bapat, P. M., Siewers, V., Goossens, K. V., et al. (2013). A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study. BIOTECHNOLOGY LETTERS, 35(6), 891–900.
- Vancouver
- 1.Van Eijsden RG, Stassen C, Daenen L, Van Mulders SE, Bapat PM, Siewers V, et al. A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study. BIOTECHNOLOGY LETTERS. 2013;35(6):891–900.
- MLA
- Van Eijsden, Rudy GE, Catherine Stassen, Luk Daenen, et al. “A Universal Fixation Method Based on Quaternary Ammonium Salts (RNAlater) for Omics-technologies: Saccharomyces Cerevisiae as a Case Study.” BIOTECHNOLOGY LETTERS 35.6 (2013): 891–900. Print.
@article{4292013,
abstract = {Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.},
author = {Van Eijsden, Rudy GE and Stassen, Catherine and Daenen, Luk and Van Mulders, Sebastiaan E and Bapat, Prashant M and Siewers, Verena and Goossens, Katty VY and Nielsen, Jens and Delvaux, Freddy R and Van Hummelen, Paul and Devreese, Bart and Willaert, Ronnie G},
issn = {0141-5492},
journal = {BIOTECHNOLOGY LETTERS},
keyword = {FROZEN,NUMBER,YEAST,PCR,DNA,METABOLISM,PRESERVATION,MESSENGER-RNA,TISSUE STORAGE METHODS,Transcriptomics,Saccharomyces cerevisiae,RNAlater,Omics,Fluxomics,Genomics},
language = {eng},
number = {6},
pages = {891--900},
title = {A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study},
url = {http://dx.doi.org/10.1007/s10529-013-1163-0},
volume = {35},
year = {2013},
}
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