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Abstract
American foulbrood is one of the most devastating diseases of the honey bee. It is caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae. The recent updated genome assembly and annotation for this pathogen now permits in-depth molecular studies. In this paper, selected techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory. Topics covered include: working with Paenibacillus larvae, basic microbiological techniques, experimental infection, and “’omics” and other sophisticated techniques. Further, this chapter covers other technical information including biosafety measures to guarantee the safe handling of this pathogen.
Keywords
pathogen, technique, disease, brood, Paenibacillus larvae, honey bee, American foulbrood, BEEBOOK, COLOSS, LARVAE SUBSP LARVAE, HONEY-BEE PATHOGEN, SUPPRESSION SUBTRACTIVE HYBRIDIZATION, GREEN FLUORESCENT PROTEIN, POLYMERASE-CHAIN-REACTION, 16S RIBOSOMAL-RNA, PAENIBACILLUS-LARVAE, BACILLUS-LARVAE, APIS-MELLIFERA, BACTERIAL PATHOGEN

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Citation

Please use this url to cite or link to this publication:

Chicago
de Graaf, Dirk, Adriana M Alippi, Karina Antunez, Katherine A Aronstein, Giles Budge, Dieter De Koker, Lina De Smet, et al. 2013. “Standard Methods for American Foulbrood Research.” Journal of Apicultural Research 52 (1).
APA
de Graaf, Dirk, Alippi, A. M., Antunez, K., Aronstein, K. A., Budge, G., De Koker, D., De Smet, L., et al. (2013). Standard methods for American foulbrood research. JOURNAL OF APICULTURAL RESEARCH, 52(1).
Vancouver
1.
de Graaf D, Alippi AM, Antunez K, Aronstein KA, Budge G, De Koker D, et al. Standard methods for American foulbrood research. JOURNAL OF APICULTURAL RESEARCH. 2013;52(1).
MLA
de Graaf, Dirk, Adriana M Alippi, Karina Antunez, et al. “Standard Methods for American Foulbrood Research.” JOURNAL OF APICULTURAL RESEARCH 52.1 (2013): n. pag. Print.
@article{4290244,
  abstract     = {American foulbrood is one of the most devastating diseases of the honey bee. It is caused by the spore-forming, Gram-positive rod-shaped bacterium Paenibacillus larvae. The recent updated genome assembly and annotation for this pathogen now permits in-depth molecular studies. In this paper, selected techniques and protocols for American foulbrood research are provided, mostly in a recipe-like format that permits easy implementation in the laboratory. Topics covered include: working with Paenibacillus larvae, basic microbiological techniques, experimental infection, and {\textquotedblleft}{\textquoteright}omics{\textquotedblright} and other sophisticated techniques. Further, this chapter covers other technical information including biosafety measures to guarantee the safe handling of this pathogen.},
  articleno    = {52.1.11},
  author       = {de Graaf, Dirk and Alippi, Adriana M and Antunez, Karina and Aronstein, Katherine A and Budge, Giles and De Koker, Dieter and De Smet, Lina and Dingman, Douglas W and Evans, Jay D and Foster, Leonard J and F{\"u}nfhaus, Anne and Garcia-Gonzalez, Eva and Gregorc, Ales and Human, Hannelie and Murray, K Daniel and Nguyen, Bach Kim and Poppinga, Lena and Spivak, Marla and vanEngelsdorp, Dennis and Wilkins, Selwyn and Genersch, Elke},
  issn         = {0021-8839},
  journal      = {JOURNAL OF APICULTURAL RESEARCH},
  language     = {eng},
  number       = {1},
  pages        = {27},
  title        = {Standard methods for American foulbrood research},
  url          = {http://dx.doi.org/10.3896/IBRA.1.52.1.11},
  volume       = {52},
  year         = {2013},
}

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