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A new role for glutathione in the regulation of root architecture linked to strigolactones

(2014) PLANT CELL AND ENVIRONMENT. 37(2). p.488-498
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Abstract
Reduced glutathione (GSH) is required for root development, but its functions are not characterized. The effects of GSH depletion on root development were therefore studied in relation to auxin and strigolactone (SL) signalling using a combination of molecular genetic approaches and pharmacological techniques. Lateral root (LR) density was significantly decreased in GSH synthesis mutants (cad2-1, pad2-, rax1-), but not by the GSH synthesis inhibitor, buthionine sulfoximine (BSO). BSO-induced GSH depletion therefore did not influence root architecture in the same way as genetic impairment. Root glutathione contents were similar in the wild-type seedlings and max3-9 and max4-1 mutants that are deficient in SL synthesis and in the SL-signalling mutant, max2-1. BSO-dependent inhibition of GSH synthesis depleted the tissue GSH pool to a similar extent in the wild-type and SL synthesis mutants, with no effect on LR density. The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings, but this increase was absent from max2-1. Taken together, these data establish a link between SLs and the GSH pool that occurs in a MAX2-dependent manner.
Keywords
ASCORBATE, PLANTS, BIOSYNTHESIS, LEAF SENESCENCE, AUXIN TRANSPORT, ARABIDOPSIS-THALIANA, GAMMA-GLUTAMYLCYSTEINE SYNTHETASE, INITIATION, redox signaling, meristem, lateral roots, hormone signalling, auxin, DIFFERENTIATION, GROWTH

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Citation

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Chicago
Marquez-Garcia, Belen, Maria Njo, Tom Beeckman, Sofie Goormachtig, and Christine H Foyer. 2014. “A New Role for Glutathione in the Regulation of Root Architecture Linked to Strigolactones.” Plant Cell and Environment 37 (2): 488–498.
APA
Marquez-Garcia, B., Njo, M., Beeckman, T., Goormachtig, S., & Foyer, C. H. (2014). A new role for glutathione in the regulation of root architecture linked to strigolactones. PLANT CELL AND ENVIRONMENT, 37(2), 488–498.
Vancouver
1.
Marquez-Garcia B, Njo M, Beeckman T, Goormachtig S, Foyer CH. A new role for glutathione in the regulation of root architecture linked to strigolactones. PLANT CELL AND ENVIRONMENT. 2014;37(2):488–98.
MLA
Marquez-Garcia, Belen, Maria Njo, Tom Beeckman, et al. “A New Role for Glutathione in the Regulation of Root Architecture Linked to Strigolactones.” PLANT CELL AND ENVIRONMENT 37.2 (2014): 488–498. Print.
@article{4288976,
  abstract     = {Reduced glutathione (GSH) is required for root development, but its functions are not characterized. The effects of GSH depletion on root development were therefore studied in relation to auxin and strigolactone (SL) signalling using a combination of molecular genetic approaches and pharmacological techniques. Lateral root (LR) density was significantly decreased in GSH synthesis mutants (cad2-1, pad2-, rax1-), but not by the GSH synthesis inhibitor, buthionine sulfoximine (BSO). BSO-induced GSH depletion therefore did not influence root architecture in the same way as genetic impairment. Root glutathione contents were similar in the wild-type seedlings and max3-9 and max4-1 mutants that are deficient in SL synthesis and in the SL-signalling mutant, max2-1. BSO-dependent inhibition of GSH synthesis depleted the tissue GSH pool to a similar extent in the wild-type and SL synthesis mutants, with no effect on LR density. The application of the SL analogue GR24 increased root glutathione in the wild-type, max3-9 and max4-1 seedlings, but this increase was absent from max2-1. Taken together, these data establish a link between SLs and the GSH pool that occurs in a MAX2-dependent manner.},
  author       = {Marquez-Garcia, Belen and Njo, Maria and Beeckman, Tom and Goormachtig, Sofie and Foyer, Christine H},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  keyword      = {ASCORBATE,PLANTS,BIOSYNTHESIS,LEAF SENESCENCE,AUXIN TRANSPORT,ARABIDOPSIS-THALIANA,GAMMA-GLUTAMYLCYSTEINE SYNTHETASE,INITIATION,redox signaling,meristem,lateral roots,hormone signalling,auxin,DIFFERENTIATION,GROWTH},
  language     = {eng},
  number       = {2},
  pages        = {488--498},
  title        = {A new role for glutathione in the regulation of root architecture linked to strigolactones},
  url          = {http://dx.doi.org/10.1111/pce.12172},
  volume       = {37},
  year         = {2014},
}

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