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Inhibition of infection and transmission of HIV-1 and lack of significant impact on the vaginal commensal lactobacilli by carbohydrate-binding agents

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Abstract
A selection of carbohydrate-binding agents (CBAs) with different glycan specificities were evaluated for their inhibitory effect against HIV infection and transmission, and their interaction with vaginal commensal bacteria. Several assays were used for the antiviral evaluation: (i) cell-free virus infection of human CD4 T lymphocyte C8166 cells; (ii) syncytium formation in co-cultures of persistently HIV-1-infected HUT-78/HIV-1 and non-infected CD4 SupT1 cells; (iii) DC-SIGN-directed capture of HIV-1 particles; and (iv) transmission of DC-SIGN-captured HIV-1 particles to uninfected CD4 C8166 cells. CBAs were also examined for their interaction with vaginal commensal lactobacilli using several viability, proliferation and adhesion assays. The CBAs showed efficient inhibitory activity in the nanomolar to low-micromolar range against four events that play a crucial role in HIV-1 infection and transmission: cell-free virus infection, fusion between HIV-1-infected and non-infected cells, HIV-1 capture by DC-SIGN and transmission of DC-SIGN-captured virus to T cells. As candidate microbicides should not interfere with the normal human microbiota, we examined the effect of CBAs against Lactobacillus strains, including a variety of vaginal strains, a gastrointestinal strain and several non-human isolates. None of the CBAs included in our studies inhibited the growth of these bacteria in several media, affected their viability or had any significant impact on their adhesion to HeLa cell monolayers. The CBAs in this study were inhibitory to HIV-1 in several in vitro infection and transmission models, and may therefore qualify as potential microbicide candidates. The lack of significant impact on commensal vaginal lactobacilli is an important property of these CBAs in view of their potential microbicidal use.
Keywords
PLANT-LECTINS, DC-SIGN, ANTIBODY 2G12, antimicrobial agents, antiretroviral therapy, AIDS, HIV, adhesion, BIOLOGICAL-ACTIVITIES, ESCHERICHIA-COLI, RHAMNOSUS GG, PRADIMICIN-S, COMPLETE GENOME SEQUENCE, URTICA-DIOICA AGGLUTININ, HUMAN-IMMUNODEFICIENCY-VIRUS, microbicides, bacterial biofilms, lactic acid bacteria

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MLA
Petrova, Mariya I, Leen Mathys, Sarah Lebeer, et al. “Inhibition of Infection and Transmission of HIV-1 and Lack of Significant Impact on the Vaginal Commensal Lactobacilli by Carbohydrate-binding Agents.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 68.9 (2013): 2026–2037. Print.
APA
Petrova, M. I., Mathys, L., Lebeer, S., Noppen, S., Van Damme, E., Tanaka, H., Igarashi, Y., et al. (2013). Inhibition of infection and transmission of HIV-1 and lack of significant impact on the vaginal commensal lactobacilli by carbohydrate-binding agents. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 68(9), 2026–2037.
Chicago author-date
Petrova, Mariya I, Leen Mathys, Sarah Lebeer, Sam Noppen, Els Van Damme, Haruo Tanaka, Yasuhiro Igarashi, Mario Vaneechoutte, Jos Vanderleyden, and Jan Balzarini. 2013. “Inhibition of Infection and Transmission of HIV-1 and Lack of Significant Impact on the Vaginal Commensal Lactobacilli by Carbohydrate-binding Agents.” Journal of Antimicrobial Chemotherapy 68 (9): 2026–2037.
Chicago author-date (all authors)
Petrova, Mariya I, Leen Mathys, Sarah Lebeer, Sam Noppen, Els Van Damme, Haruo Tanaka, Yasuhiro Igarashi, Mario Vaneechoutte, Jos Vanderleyden, and Jan Balzarini. 2013. “Inhibition of Infection and Transmission of HIV-1 and Lack of Significant Impact on the Vaginal Commensal Lactobacilli by Carbohydrate-binding Agents.” Journal of Antimicrobial Chemotherapy 68 (9): 2026–2037.
Vancouver
1.
Petrova MI, Mathys L, Lebeer S, Noppen S, Van Damme E, Tanaka H, et al. Inhibition of infection and transmission of HIV-1 and lack of significant impact on the vaginal commensal lactobacilli by carbohydrate-binding agents. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2013;68(9):2026–37.
IEEE
[1]
M. I. Petrova et al., “Inhibition of infection and transmission of HIV-1 and lack of significant impact on the vaginal commensal lactobacilli by carbohydrate-binding agents,” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 68, no. 9, pp. 2026–2037, 2013.
@article{4283492,
  abstract     = {A selection of carbohydrate-binding agents (CBAs) with different glycan specificities were evaluated for their inhibitory effect against HIV infection and transmission, and their interaction with vaginal commensal bacteria.
Several assays were used for the antiviral evaluation: (i) cell-free virus infection of human CD4 T lymphocyte C8166 cells; (ii) syncytium formation in co-cultures of persistently HIV-1-infected HUT-78/HIV-1 and non-infected CD4 SupT1 cells; (iii) DC-SIGN-directed capture of HIV-1 particles; and (iv) transmission of DC-SIGN-captured HIV-1 particles to uninfected CD4 C8166 cells. CBAs were also examined for their interaction with vaginal commensal lactobacilli using several viability, proliferation and adhesion assays.
The CBAs showed efficient inhibitory activity in the nanomolar to low-micromolar range against four events that play a crucial role in HIV-1 infection and transmission: cell-free virus infection, fusion between HIV-1-infected and non-infected cells, HIV-1 capture by DC-SIGN and transmission of DC-SIGN-captured virus to T cells. As candidate microbicides should not interfere with the normal human microbiota, we examined the effect of CBAs against Lactobacillus strains, including a variety of vaginal strains, a gastrointestinal strain and several non-human isolates. None of the CBAs included in our studies inhibited the growth of these bacteria in several media, affected their viability or had any significant impact on their adhesion to HeLa cell monolayers.
The CBAs in this study were inhibitory to HIV-1 in several in vitro infection and transmission models, and may therefore qualify as potential microbicide candidates. The lack of significant impact on commensal vaginal lactobacilli is an important property of these CBAs in view of their potential microbicidal use.},
  author       = {Petrova, Mariya I and Mathys, Leen and Lebeer, Sarah and Noppen, Sam and Van Damme, Els and Tanaka, Haruo and Igarashi, Yasuhiro and Vaneechoutte, Mario and Vanderleyden, Jos and Balzarini, Jan},
  issn         = {0305-7453},
  journal      = {JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY},
  keywords     = {PLANT-LECTINS,DC-SIGN,ANTIBODY 2G12,antimicrobial agents,antiretroviral therapy,AIDS,HIV,adhesion,BIOLOGICAL-ACTIVITIES,ESCHERICHIA-COLI,RHAMNOSUS GG,PRADIMICIN-S,COMPLETE GENOME SEQUENCE,URTICA-DIOICA AGGLUTININ,HUMAN-IMMUNODEFICIENCY-VIRUS,microbicides,bacterial biofilms,lactic acid bacteria},
  language     = {eng},
  number       = {9},
  pages        = {2026--2037},
  title        = {Inhibition of infection and transmission of HIV-1 and lack of significant impact on the vaginal commensal lactobacilli by carbohydrate-binding agents},
  url          = {http://dx.doi.org/10.1093/jac/dkt152},
  volume       = {68},
  year         = {2013},
}

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